49 results about "Cellular oxygen" patented technology

A probe for sensing and imaging intracellular oxygen comprises an oxygen-sensitive fluorescent or phosphorescent dye combined with a hydrophilic macromolecular carrier and a cell loading agent. A method for sensing cellular oxygen using the probe is also described.

Provide herein are compounds and pharmaceutical compositions suitable as modulators of hemoglobin, methods and intermediates for their preparation, and methods for their use in treating disorders mediated by hemoglobin and disorders that would benefit from tissue and / or cellular oxygenation.

Provide herein are compounds and pharmaceutical compositions suitable as modulators of hemoglobin, methods and intermediates for their preparation, and methods for their use in treating disorders mediated by hemoglobin and disorders that would benefit from tissue and / or cellular oxygenation.

The present invention encompasses the use of compounds for a novel approach to treat and prevent diseases, conditions, and disorders such as diabetes and ischemic reperfusion injury. Compounds of the invention, including but not limited to BAM15((2-fluorophenyl){6-[(2-fluorophenyl)amino](1,2,5-oxadiazolo[3,4-e]pyrazin-5-yl)}amine), a mitochondrial uncoupler, can improve glucose tolerance, increases cellular oxygen consumption, treat or prevent kidney ischemia reperfusion injury reverse insulin resistance, reverse or treat hyperinsulinemia, and reverse or treat hyperlipidemia. The present invention further provides novel compounds as well as methods for identifying compounds with the same or similar properties as BAM15.

A device and system that allows an inverted microscope with a movable x-y stage to also function as a time-resolved plate reader. The device, which is sized to fit into the condenser holder of an inverted microscope, not only provides plate-reading function at very low cost compared to that of purchasing a plate reader, but it also allows the correlation of plate reader measurements with images of a specimen. The device and system is also able to measure other parameters associated with cell metabolism, such as pH changes.

The invention discloses a cellular oxygen carrier chemical-looping reforming reactor. The cellular oxygen carrier chemical-looping reforming reactor comprises a reactor main body which comprises an outer wall and an outer heating barrel, wherein cellular oxygen carriers are formed in the outer wall and the outer heating barrel and are jointed with the outer wall and the inner wall of the outer heating barrel, and cellular pore paths parallel to the outer wall and a main shaft of the outer heating barrel are arranged in the cellular oxygen carrier and are pairwise spaced; and the tops of the outer wall and the outer heating barrel are connected with a top gas collecting region through upper flanges, the bottoms of the outer wall and the outer heating barrel are connected with a bottom gas storage region through lower flanges, a tail gas outlet is connected outside the top gas collecting region, a tee-junction gas inlet is connected outside the bottom gas storage region, and the bottom gas storage region is internally provided with a radiation type gas pre-distribution device connected with the tee-junction gas inlet. The cellular oxygen carriers have the advantages of having heat storage function, being capable of helping the reactor to maintain reaction temperature, alleviating the thermal load of external heating equipment and being beneficial to the stable proceeding of reaction.

The invention belongs to the technical field of molecular biology, and relates to a method for screening traditional Chinese medicine monomers, specifically relating to a method for screening monomer ingredients which have protective effect for oxidative damages to liver cells from Chinese traditional medicines by using mitochondrion index technique. In the invention, mitochondrial function detection is used as the core technology and methods of cell proliferation detection, mitochondrial reactive oxygen species generation detection, mitochondrial membrane potential detection, energy substance ATP in mitochondria detection and mitochondrial cytochrome C detection are adopted. Mitochondrial function changes are detected after H7702 cells, which are subject to oxidative damages induced by H2O2, are treated by the traditional Chinese medicine monomers of SalB, protocatechualdehyde, sodium danshensu, cordyceps polysaccharose, cordycepin and cordycepic acid, and the traditional Chinese medicine monomers, which have protective effect for oxidative damages to liver cells, are screened. The invention further provides applications of the traditional Chinese medicine monomers screened in preparing drugs for preventing and treating liver diseases such as fatty liver, viral hepatic diseases and liver fibrosis.

A microplate apparatus having a bottom plate defining a plurality of very small microwells (for example, each having a volume of 0.02 or less) for containing sample material and a top plate defining a plurality of domes, each of which housing a sensors for measuring biological parameters, such as cellular oxygen consumption, pH changes, calcium uptake, and metabolic reduction of fluorescent dyes. The top and bottom plates may be composed of quartz. The microplate assembly may be fabricated using a method involving applying an etching solution to each of the top and bottom plates to create the domes and microwells. Additionally, etching solution may be applied to the bottom plate to create a raised lip region around each of the microwells. The etched-away areas surrounding the lip regions facilitate the changing of microwell solution without disrupting the samples.

The invention provides application of vaccarin for resisting oxidation and high-glucose damage, and belongs to the technical field of traditional Chinese medicament application. The application first discovers that vaccarin has the activity of protecting oxidative stress injury and high-glucose damage of endothelial cells and can be used for treating a series of diseases induced by atherosclerosis, cataract, aged-related macular degeneration and other endogenous or exogenous active oxygen invasions, and preventing and treating diabetes mellitus, hypertension and related diseases caused by high glucose. The vaccarin can be applied to medicinal preparations, nourishment, healthy products, cosmetics and the like.

The invention belongs to the technical field of nutrition products and particularly relates to a composition for improving glutathione concentration in a human body and a preparation method and an application thereof. The composition is composed of an A component and a gain agent, wherein the A component is composed of glutamic acid and / or glutamic acid derivatives, cystine and / or cystine derivatives, glycine and / or glycine derivatives. According to the composition disclosed by the invention, the A component is cooperatively used together with the gain agent to improve the solubility of the composition in the mouth, so as to promote the adsorption of a human body to the composition, improve the utilization rate of the composition, more effectively promote the synthesis of glutathione in vivo and improve the concentration level of the glutathione in the human body and further improve the immunity of the human body; and the composition disclosed by the invention has antioxygenation and detoxication. The combined use of the A component and the gain agent can also improve the taste of the composition, so that the composition is more suitable for direct taking of people. Since the glutathione is a very important antioxidant in the human body, and cellular oxidation is the root of aging and various diseases, the composition disclosed by the invention can be applied to prevention and auxiliary treatment of various diseases.

The invention relates to the technical field of cosmetics, in particular to an antioxidative composition and a preparation method and application thereof. In the composition, fructus schizandrae and soybeans are compounded, an experiment shows that the composition has a remarkable clearing effect to DPPH free radicals, cell oxidation damage caused by t-Bhp stimulation can be reduced remarkably, growth of cells formed by keratoderma is protected effectively, and an obvious synergistic antioxidative effect is achieved.

The invention discloses application of melatonin in promotion of cardiac stem cell transplanting for treating myocardial infarction. The application is characterized in that by performing in-vitro culture on cardiac stem cells (CPCs), the CPCs are pretreated for 2h by the melatonin of different concentrations, and then 150muM H2O2 (hydrogen peroxide) is lately added to simulate the oxidative stress loss of cells; after 24h, an Edu dyeing is used for detecting the influence of the melatonin to the cell proliferation of the CPCs, and a Tunnel experiment is used for detecting the cell withering;in addition, the CPCs are cultured in vitro, treated by 10muM melatonin, and injected into the heart of a mouse with myocardial infarction, and the heart function of the mouse is detected by a B-typeultrasonic Doppler instrument; found by results, the melatonin can effectively resist the oxidative stress loss of the CPCs, increase the cell proliferation ability of the injured CPCs, and inhibit the withering, and meanwhile, the melatonin has the function of promoting the cardiac stem cell transplanting for treating the myocardial infarction. The application has the advantages that the treatment effect of a patient with myocardial infarction is certainly improved, the disease and death rate is reduced, and a new technical means is provided for the treatment of the myocardial infarction.

The invention discloses aesculetin with anti-oxidative and anti-aging activity as well as a preparation method and an application of aesculetin. The preparation method comprises preparation steps as follows: (1), cortex fraxini residues are dried, sterilized for 30 min under ultraviolet irradiation and taken out; (2), distilled water which is 8 times the sterilized cortex fraxini residues in parts by weight is added to the sterilized cortex fraxini residues for fermentation, after fermentation, a sample is taken out, dried, smashed and screened by a 30-mesh sieve, and fermented cortex fraxini residue powder is obtained; (3), an organic extracting agent is added to the fermented cortex fraxini residue powder for reflux extraction, extracting solutions are combined, a combined solution is filtered, and the organic extracting agent is subjected to reduced-pressure recovering and concentrated into thick paste; (4), distilled water is added to the thick paste for dilution, heating dissolution is performed, an obtained solution is filtered while still hot, and a filtrate is separated, purified and decolorized and then is subjected to reduced-pressure concentration and freeze drying finally. Aesculetin has a function of eliminating oxygen free radicals, has a protecting effect on cell DNA oxidative damage caused by lipid peroxide and can effectively delay skin cell senescence.

A microplate apparatus having a bottom plate defining a plurality of very small microwells (for example, each having a volume of 0.02 or less) for containing sample material and a top plate defining a plurality of domes, each of which housing a sensors for measuring biological parameters, such as cellular oxygen consumption, pH changes, calcium uptake, and metabolic reduction of fluorescent dyes. The top and bottom plates may be composed of quartz. The microplate assembly may be fabricated using a method involving applying an etching solution to each of the top and bottom plates to create the domes and microwells. Additionally, etching solution may be applied to the bottom plate to create a raised lip region around each of the microwells. The etched-away areas surrounding the lip regions facilitate the changing of microwell solution without disrupting the samples.

The invention provides a method for applying L-selenomethionine to animal cell antioxidation and a product thereof, which relate to the technical field of animal cell culture. The method comprises thefollowing step of adding an L-selenomethionine working solution into a culture system of animal cells so that the final concentration of L-selenomethionine in the culture system of animal cells is 0.5-1.5 mu M; wherein the concentration of the L-selenomethionine working solution is 5-15 [mu] M. The application method of L-selenomethionine in animal cell antioxidation alleviates the lack of an animal cell antioxidation method which can effectively reduce cell oxidative damage and maintain cell activity, and is low in cytotoxicity, simple and convenient to operate and low in cost in the prior art. The invention also provides an animal cell antioxidant containing the L-selenomethionine, an animal cell culture medium containing the L-selenomethionine or the animal cell antioxidant containingthe L-selenomethionine, and a medicine or a feed additive containing the L-selenomethionine or the animal cell antioxidant containing the L-selenomethionine.

The invention discloses a porcine epithelial cell oxidative stress model, an establishment method therefor and application of the porcine epithelial cell oxidative stress model. The establishment method for the porcine epithelial cell oxidative stress model comprises the following steps: inducing porcine epithelial cells by AAPH to continuously generate stable endogenous free radicals, detecting the oxidative damage degree of the cells, and determining a stress model of oxidative damage. According to the porcine epithelial cell oxidative stress model, the optimal concentration for constructingthe porcine small intestinal epithelial cell oxidative stress model induced by the AAPH is determined by a series of tests, and the oxidative damage is evaluated by the established oxidative stress model, so that the convenient and stable intestinal epithelial cell model is provided for further research of the influence of the oxidative stress on animal intestinal health, and the model has important significance for maintaining human health and reducing direct losses and indirect losses caused by oxidative stress in animal husbandry production.

The invention discloses a method for preparing a deproteinized calf blood extract. The method comprises the following steps: (a) performing negative catalysis; (b) performing acid precipitation; (c) freezing at a low temperature, raising the temperature, dissolving, centrifuging, and taking the supernatant; (d) performing alkali precipitation; (e) repeating the step (c); (f) performing alcohol precipitation and centrifuging the supernatant in the step (e), and taking the supernatant; (g) performing ultrafiltration and vacuum concentration on the supernatant in the step (f), thereby obtaining concentrated ultrafiltrate; and (h) performing ultrafiltration on the concentrated ultrafiltrate in the step (g), collecting the ultrafiltrate, thereby obtaining the product. According to the preparation method, the biological activity is not damaged, exogenous ions are not introduced, and hybrid proteins in the extract can be effectively removed. The injection prepared from the deproteinized calf blood extract does not cause anaphylactic reaction, is high in safety, and can effectively treat brain cell metabolic disorder diseases such as cerebral apoplexy, cerebral trauma, cerebral insufficiency and cerebral dementia and improve the cell oxygen content and microcirculation. According to a preparation method of the injection, the quality stability and safety of a final product can be fully guaranteed, a medicinal effect of the preparation is guaranteed, and side effects of the conventional preparation are eliminated.

Provided is a cell culture device that has a simple structure and ensures sufficient nutrient supply to cells and oxygenation of the cells to thereby enable mass cell culture. The cell culture device comprises: a cell culture container which is an approximately cylindrical body provided with a flat bottom part at the lower end; a dish-shaped body having an approximately disc shape which is provided with a plurality of magnetic attraction members, said magnetic attraction members being positioned in the circumferential part at equal intervals, and horizontally disposed in a non-contact state within a hollow space inside the cell culture container; and a cyclic body which is provided with a plurality of magnetic attraction members and positioned outside the cell culture container so that the cell culture container is located within the cycle thereof. The cyclic body and the dish-shaped body move vertically in magnetic conjunction to thereby agitate a medium and supply nutrients to cells.

This invention relates to dietary compositions and to methods for modifying cellular metabolism, metabolic production of reactive oxygen species and the resulting level of reactive oxygen species. The invention is drawn to a composition comprising a combination of carnitine, lipoic acid and polyphenol, which has the effect of enhancing metabolism and reducing oxygen species at the same time. When the composition is fed to older animals, the animals will have improved metabolism at the cellular level and a reduction of oxidative stress. The invention is also drawn to a method of oral administration of carnitine, lipoic acid and polyphenol to a mammalian host, at an effective dose necessary to affect enhanced metabolic processes and reduced oxygen species in animals including humans.

The present invention relates to a method for preventing cancer by using a phthalide compound, wherein the phthalide compound has an effect of increasing the oxygen release efficiency of hemoglobin (Hb) of a subject to increase the oxygenation level of organs and tissue cells, thereby preventing the cellular oxygenation level against falling below the critical cellular oxygen requirements under which the normal cells can turn cancerous. Although cancer may be caused by a variety of reasons, including congenital inheritance, external environment, air pollution or poor living and dietary habits, there is only one primary and common reason in causing cancers, the excessively low cellular oxygenation level. When the oxygenation level of any cell falls below 60% of its physiological oxygen requirements, the normal cell may turn cancerous. The present invention is to use the phthalide compound to substitute for or cooperate with 2,3-BPG of a subject to lower the oxygen affinity of hemoglobin (Hb) and to facilitate the release of oxygen from hemoglobin to tissue cells, as a result, the cellular oxygenation level is maintained always higher than the critical threshold for the normal cells to turn cancerous, and by doing so to prevent the normal cells from turning cancerous and prevent the developments of cancers.

The invention provides an application of donkey-hide gelatin in a product for relieving cell oxidative stress damage, and relates to the technical field of biopharmaceuticals. The donkey-hide gelatin is used to prepare products that can regulate the expression of genes in cultured objects, and the genes that can be regulated include genes related to oxidative stress damage repair and stress response. Applying donkey-hide gelatin to the culture can alleviate the changes of oxidative stress damage on the gene expression level of the cultured subjects.

The invention discloses a method for preparing a protein-free extract of calf blood, which comprises the following steps: a) slowing down; b) acid precipitation; c) low-temperature freezing, heating up for dissolution, centrifugation, and supernatant; d) alkali precipitation ; e) repeating c) step; f) ethanol precipitation and centrifugation of the supernatant of step e), and taking the supernatant; g) ultrafiltration and vacuum concentration of the supernatant of step f) to obtain concentrated ultrafiltrate; h) ultrafiltering the concentrated ultrafiltrate in step g), and collecting the ultrafiltrate to obtain. The preparation method provided by the present invention neither destroys biological activity nor introduces exogenous ions, and can effectively remove impurities therein; the injection prepared from the deproteinized calf blood extract will not cause allergic reactions and is safe. High, can effectively treat brain cell metabolic disorders such as stroke, traumatic brain injury, brain insufficiency and brain dementia, improve cellular oxygen content and microcirculation; the preparation method of the injection can fully guarantee the quality stability and safety of the final product , to ensure the medicinal effect of the preparation, and to eliminate the side effects of the existing preparation.

The invention provides application of an xenicane type diterpenoid compound epoxyhydroxyacetyldictyolal to preparation of antioxidative neuroprotective drugs. The diterpenoid compound epoxyhydroxyacetyldictyolal can significantly inhibit the neuronal PC12 cell oxidative damage induced by H2O2, promote PC12 cell colony formation, and inhibit release of lactate dehydrogenase (LDH). Diterpene is an excellent antioxidant, has the protective effect on the oxidative damage of nerve cells, and can be used for preventing or treating the cerebral ischemia-reperfusion injury.

The invention belongs to the field of medicinal chemistry. Aiming at the patent medicine defects that curcumin is unstable, and mono-ketene mono-carbonyl curcumin analogues are high in toxicity and low in stability, the inventionparticularly provides application of a mono-ketene mono-carbonyl curcumin analogue which is stable in structure and good in safety in drugs, especially application of the analogue used as an antioxidant drug in treatment of diseases caused by oxidative stress. The compound has good in-vitro and in-vivo anti-oxidation effects; in vitro, the compound has an excellent anti-oxidation protection effect in a hydrogen peroxide induced cell oxidative damage model; and in a mouse body, in oxidative stress related cerebral ischemia reperfusion injury, the compound has a good anti-oxidation protection effect.