Porcine epithelial cell oxidative stress model, establishment method therefor and application of porcine epithelial cell oxidative stress model

Abstract

The invention discloses a porcine epithelial cell oxidative stress model, an establishment method therefor and application of the porcine epithelial cell oxidative stress model. The establishment method for the porcine epithelial cell oxidative stress model comprises the following steps: inducing porcine epithelial cells by AAPH to continuously generate stable endogenous free radicals, detecting the oxidative damage degree of the cells, and determining a stress model of oxidative damage. According to the porcine epithelial cell oxidative stress model, the optimal concentration for constructingthe porcine small intestinal epithelial cell oxidative stress model induced by the AAPH is determined by a series of tests, and the oxidative damage is evaluated by the established oxidative stress model, so that the convenient and stable intestinal epithelial cell model is provided for further research of the influence of the oxidative stress on animal intestinal health, and the model has important significance for maintaining human health and reducing direct losses and indirect losses caused by oxidative stress in animal husbandry production.

Description

technical field [0001] The invention belongs to the technical field of cell model establishment, and in particular relates to a porcine epithelial cell oxidative stress model and its establishment method and application. Background technique [0002] In recent years, with the rapid development of my country's animal husbandry industry, the problem of stress response in livestock and poultry has become increasingly serious. A large number of studies have shown that the stress of livestock and poultry can cause a variety of diseases, which seriously restricts the development of the breeding industry. During the breeding process, many factors, such as environmental changes, nutrient deficiency, trace element deficiency, high intake of polyunsaturated fatty acids, and the use of chemicals, may cause oxidative stress (oxidative stress, OS) in livestock and poultry. In the state of oxidative stress, the dynamic balance between the production and scavenging mechanism of oxygen fre...

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Problems solved by technology

[0010] The purpose of the present invention is to provide a porcine epithelial cell oxidative stress model and its establishment method and application thereof, aiming to solve the problems existing in the process of establishing the cell oxidative stress model with the commonly used drugs that directly release free radicals. Various problems described in

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