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Application of vaccarin for resisting oxidation and high-glucose damage

A technology of flavonoid glycosides and anti-oxidation of Wang Bu Liuxing, which is applied in the directions of medical preparations containing active ingredients, applications, organic active ingredients, etc.

Active Publication Date: 2014-07-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After investigation, there is no relevant literature report (from CNKI, Weipu China Journal Network) that Chinese scholars have found that flavonoid glycosides of blalicius can protect the endothelium from oxidative stress damage. Research fields such as pharmacology and drug efficacy of sugar damage are still in a blank stage

Method used

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  • Application of vaccarin for resisting oxidation and high-glucose damage
  • Application of vaccarin for resisting oxidation and high-glucose damage
  • Application of vaccarin for resisting oxidation and high-glucose damage

Examples

Experimental program
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Effect test

Embodiment 1

[0008] Embodiment 1: the effect of flavonoid glycosides of wangbuliuxing on H 2 o 2 Induced EA·hy926 cell injury improvement

[0009] Select cells in logarithmic growth phase, trypsinize to make cell suspension, adjust cell concentration, inoculate 8000 cells per well in 96-well plate, inoculate 160 μL in each well, set 4 duplicate wells in each group, and randomly group them after 24 hours : The normal group and the model group were added with 20 μL of serum-free medium, and the three drug groups were respectively added with the corresponding concentration and equal volume of flavonoid glucoside liquid, so that the final concentrations of the drug were 13.76, 6.88, and 3.44 μmol / L respectively. After pre-protection incubation at 37°C for 12 hours, 20 μL of H 2 o 2 , the final concentration of which was 1000 μmol / L. The normal control group was added with 20 μL of serum-free medium, incubated at 37°C for 2 hours, and the cell viability was detected by SRB method. See Table...

Embodiment 2

[0019] Example 2: Improvement of high glucose-induced EA·hy926 cell damage by flavonoid glycosides

[0020] Select cells in the logarithmic growth phase, trypsinize to make cell suspension, adjust the cell concentration, inoculate 8000 cells per well in a 96-well plate, inoculate 160 μL in each well, and randomly divide into groups after 24 hours: normal group and model group add 20 μL In the serum-free medium, the 3 administration groups were respectively added with the corresponding concentration and equal volume of flavonoid glucoside liquid, so that the final concentration of the drug was 13.76, 6.88, and 3.44 μmol / L respectively, and incubated at 37°C for 12 hours after pre-protection, Add 20 μL of glucose (final concentration: 180 mmol / L) to each well of the model group and the blancillin group, add 20 μL of serum-free medium to the normal control group, set up 4 duplicate wells in each group, incubate at 37°C for 24 hours, and detect with SRB method cell viability. See...

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Abstract

The invention provides application of vaccarin for resisting oxidation and high-glucose damage, and belongs to the technical field of traditional Chinese medicament application. The application first discovers that vaccarin has the activity of protecting oxidative stress injury and high-glucose damage of endothelial cells and can be used for treating a series of diseases induced by atherosclerosis, cataract, aged-related macular degeneration and other endogenous or exogenous active oxygen invasions, and preventing and treating diabetes mellitus, hypertension and related diseases caused by high glucose. The vaccarin can be applied to medicinal preparations, nourishment, healthy products, cosmetics and the like.

Description

Technical field: [0001] The invention relates to an application of bulicurin flavonoid glycosides capable of resisting oxidation damage and high glucose damage, and belongs to the technical field of traditional Chinese medicine application. The invention relates to a flavonoid glycoside with anti-oxidation and anti-high sugar damage activity, and clarifies its new application in medicine, food and cosmetics. Background technique: [0002] Wangbuliu is the dry mature seed of Vaccaria segetalis (Neck.) Garcke, a plant of Caryophyllaceae. Buliuxing mainly contains triterpenoid saponins, flavonoid glycosides, cyclic peptides, lipids, fatty acids, monosaccharides and other components [1, Li Fan, Liang Jingyu. Research progress on blanc blanc[J]. Strait Pharmacy, 2007, 19(3):1-5], the flavonoid glycosides of blancalis are light yellow granular crystals, easily soluble in methanol, methanol-water, ethanol, ethanol-water, n-butanol, etc., insoluble in chloroform, ethyl acetate Est...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A23L1/09A61K8/60A61P9/10A61P3/10A61P27/12A61P27/02A61P9/12
Inventor 邱丽颖谢凤珊冯磊杜斌蔡维维李曰金坚
Owner JIANGNAN UNIV
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