A method for screening traditional Chinese medicine monomers
A traditional Chinese medicine, monomer technology, applied in the field of molecular biology, can solve problems such as liver cell damage
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[0076] (1) Establishment of cell oxidative damage model and grouping of traditional Chinese medicine monomer effects
[0077] 1. Main reagents:
[0078] High glucose medium (DMEM) was purchased from Invitrogen Company, and calf serum was purchased from Shanghai Shisheng Company. Salvianolic acid B, danshensu sodium, protocatechualdehyde, cordyceps polysaccharide, cordycepin, cordycepic acid and other traditional Chinese medicine monomers were provided by Shanghai University of Traditional Chinese Medicine.
[0079] 2. Experimental steps:
[0080] Take the H7702 cells in the logarithmic growth phase, make a single cell suspension, and use 5×10 4 Cells / ml were inoculated into 96-well culture plates, and cultured in 200 μl of culture medium at 37° C. and 5% CO2 incubator for 12 hours. Join H 2 o 2 Make the final concentration 400μM, continue to culture for 2h, and establish the oxidative damage model.
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