34 results about "Cartilaginous Differentiation" patented technology

The invention relates to a cell co-culture system for promoting cartilage differentiation.The cell co-culture system is composed of synovial-derived mesenchymal stem cells and meniscus cells, the proportion of the synovial-derived mesenchymal stem cells to the meniscus cells is 1: (0.33-3), the synovial-derived mesenchymal stem cells are 2-5 -generation cells which are subjected to primary culture, and the meniscus cells are 2-5 -generation cells which are subjected to primary culture; the system can jointly form a meniscus transplantation system with demineralization cortical bone and a BMP-4-PBS solution; the invention further provides application of the cell co-culture system to meniscus transplantation of allogeneic or xenogenic animals; the cell co-culture system effectively promotes cartilage differentiation, and the success rate of meniscus transplantation surgery is raised.

The present invention is to provide a 3D cartilage organoid block prepared by differentiating mesenchymal stem cells into 3D spheroid cartilage tissues, a basic unit for the 3D cartilage spheroid block. The inventors found that both the amount of GAG matrix and the expression of the collagen type2 increased. Therefore, the method of this invention provides clinically applicable cartilage tissues by effectively enhancing the function of the cartilage differentiation constructs according to 2D culture. The 3D cartilage organoid block can be usefully applied to the area, such as, articular cartilage regeneration and plastic aurgery, where cartilage tissues restoration is required.

The invention discloses an application of LncRNA-32598 in the preparation of a drug or gene delivery system for inhibiting chondrocyte hypertrophic differentiation. A nucleic acid sequence of LncRNA-32598 is as shown in SEQ ID NO.1, and cytology experiments show that LncRNA-32598 can inhibit hypertrophy of chondrocytes, can be used to inhibit a hypertrophic process of MSCs after chondrogenic differentiation and maintain the matrix metabolism balance of chondrocytes, and has good application prospects in tissue engineering cartilage construction and cartilage defect repair, and intervention ofcartilage matrix degeneration in osteoarthritis.

The invention belongs to the field of biomedical materials and particularly relates to a preparation method of a co-crosslinked double-network hydrogel support for promoting cartilage repair and growth. The method comprises the following steps of (1) preparation of GelMA; (2) preparation of a photo-crosslinked polypeptide; and (3) preparation of a hydrogel support, namely preparing the hydrogel support from the GelMA and the photo-crosslinked HSNGLPL under the action of a photoinitiator. The prepared hydrogel support has an obvious effect of promoting new cartilage generation and cartilage defect healing, and is capable of regulating cartilage matrix secretion of normal chondrocytes around the injured tissue, mesenchymal stem cell migration to the injured part and cartilage differentiation; by virtue of a porous structure of the hydrogel support, the HSNGLPL can be crosslinked to the surface of a porous support, endogenous TFG-beta is effectively adsorbed, the concentration of local TFG-beta beta1 is improved and the effects of the biological support in the orthopedics fields, such as a cartilage defect and a cartilage injury are achieved.

The invention discloses a tissue-engineered bone based on an entochondrostosis system and a construction method thereof. The construction method is characterized in that mesenchymal stem cells are cultivated on a porous bone scaffold material to construct a tissue-engineering complex, subjected to in vitro cartilage differentiation induction cultivation for two weeks and continuously conducted with fat cartilage differentiation induction cultivation for two weeks to obtain the tissue-engineered bone. According to the invention, an in vivo research result shows that the in vivo ectopic bone formation can be realized, bone defects can be successfully repaired, the difficulties that the uniform vascularization of large piece of tissue-engineered bone cannot be realized and enough nutrition can't be supplied are solved, and the construction method has a very good application prospect in the construction of the tissue-engineered bone and bone defect repairing.

The invention discloses a temperature-sensitive collagen-based hydrogel loaded with biologically active polypeptides for repairing osteoarticular cartilage defects and a preparation method thereof. The thermosensitive collagen-based hydrogel is made of N-vinylcaprolactam, methacrylic acid, recombinant human type III collagen, bioactive peptides and PLGA, etc. It has good biocompatibility, strong cell attachment ability, It is non-toxic, degradable and non-immunogenic, and has a good repairing effect on animal cartilage defects. It utilizes the injectability of thermosensitive collagen-based hydrogel, the elasticity-enhancing potential of type III collagen and the potential of promoting cartilage differentiation, combined with the promotion effect of bioactive peptides on the repair of articular cartilage damage, making it a new product that meets the current needs. It is a tissue regeneration material that is excellently suitable for the repair and treatment of clinical cartilage defects.

The invention belongs to the field of polymer functional materials, specifically zwitterionic hydrogel capable of recruiting type II collagen and its preparation method and application. The zwitterionic hydrogel realizes the chemistry of type II collagen-binding polypeptide WYRGRL by introducing co-polymerized active ester monomers. Modified, prepared by UV cross-linking. This hydrogel can specifically bind type II collagen in cartilage components. When co-cultured with chondrocytes or bone marrow mesenchymal stem cells, it can preferentially capture a large amount of type II collagen in situ, thereby promoting the process of cartilage differentiation and improving hydraulic coagulation. Gum cartilage repair ability. At the same time, the lubricity provided by the zwitterionic monomer can avoid the wear of the hydrogel during high-shear motion. This zwitterionic hydrogel with II collagen recruitment function has broad application prospects in bone tissue engineering.

This invention provides a method for administration of an effective amount of RANKL-binding molecules that act on prechondrocytes and / or mesenchymal stem cells, accelerate cartilage differentiation, proliferation, and maturation of such cells, enhance chondrocyte differentiation, and induce chondrocyte proliferation to induce chondrocyte proliferation and differentiation or increase cartilage matrix production and a pharmaceutical composition used for inducing chondrocyte proliferation and differentiation or increasing cartilage matrix production. The pharmaceutical composition used for treatment or prevention of a chondropathies comprises, as an active ingredient, a compound that acts on prechondrocytes and / or mesenchymal stem cells and induces at least one of the following: (a) acceleration of prechondrocyte and / or mesenchymal stem cell differentiation; (b) acceleration of prechondrocyte and / or mesenchymal stem cell proliferation; (c) acceleration of prechondrocyte and / or mesenchymal stem cell maturation; (d) enhancement of chondrocyte differentiation; (e) chondrocyte proliferation; and (f) increased production of the cartilage matrix.

The invention belongs to the technical field of tissue-inductive biomaterial preparation, and in particular relates to a preparation method and application of a cartilage-inductive matrix material. The preparation method of the cartilage inductive matrix material provided by the invention is to depilate, divide, degrease and remove heavy metals from the calfskin; then perform sterilization, acid soaking, and enzyme digestion to obtain the collagen solution; finally, the collagen solution is salted. Analysis, purification, dialysis, neutralization, that is. The matrix material prepared by the preparation method of the cartilage inductive matrix material provided by the present invention has the advantages of high biological activity, good biocompatibility, biodegradability and low antigenicity, and the matrix material is used as a stem cell carrier to induce its Chondrogenic differentiation can realize the repair and regeneration of cartilage defects, and can also be used as tissue engineering scaffold materials and soft tissue filling materials.

The invention provides a mesenchymal stem cell membrane camouflaged magnetic drug-carrying nanoparticle and a preparation method and application thereof. The drug-carrying nanoparticle comprises drug-carrying Fe 3 O 4 Nanoparticles and composites in drug-loaded Fe 3 O 4 Mesenchymal stem cell membrane on nanoparticle surface; drug-loaded Fe 3 O 4 Drugs and Fe in Nanoparticles 3 O 4 The mass ratio of nanoparticles is 1:4.5 to 5.5; the drug is selected from drugs that promote cartilage differentiation and repair; drug-loaded Fe 3 O 4 The ratio of the mass of nanoparticles to the number of cells derived from the mesenchymal stem cell membrane is (3.4-3.9) mg:5×10 6 indivual. The drug-loaded nanoparticles use the mesenchymal stem cell membrane as a carrier, and will be loaded with Fe-containing drugs that promote cartilage differentiation and repair. 3 O 4 The nanoparticle coating can stably and efficiently transport the drug to the lesion site, so that it can promote the repair of cartilage tissue. It is non-toxic, degradable, and has good biocompatibility; it is better than simple topical medication to promote cartilage repair.