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Zwitterionic hydrogel capable of recruiting type II collagen and its preparation method and application

A zwitterion, hydrogel technology, applied in the direction of prosthesis, peptide, medical science, etc., can solve the problem of not being able to recruit type II collagen, and achieve the effect of easy mass production and application promotion, improved repair effect, and simple preparation process.

Active Publication Date: 2022-04-08
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used hydrogels for cartilage repair are mainly connected to the chondrogenic differentiation behavior of bone marrow mesenchymal stem cells by adding chondrogenic differentiation factors, but they cannot recruit type II collagen secreted by chondrocytes.

Method used

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  • Zwitterionic hydrogel capable of recruiting type II collagen and its preparation method and application
  • Zwitterionic hydrogel capable of recruiting type II collagen and its preparation method and application
  • Zwitterionic hydrogel capable of recruiting type II collagen and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In this embodiment, the preparation method of active ester functional monomer is provided, and the steps are as follows:

[0038] Dissolve N-hydroxysuccinimide (NHS, 1.1736g, 12mmol) and triethylamine (1,650μL, 12mmol) in 5mL of chromatographic grade dichloromethane, and stir for 30 minutes under ice-bath conditions. At the same time, methyl Acryloyl chloride (1 mL, 11 mmol) was dissolved in 5 mL of chromatographic grade dichloromethane and added dropwise to the previously prepared solution at a rate of 1 drop per 10 seconds. After the solution was added dropwise, the reaction system was gradually returned to room temperature and the reaction was continued for 12 h. After the reaction, wash with distilled water 3 times, 5 mL each time; then dry with anhydrous sodium sulfate, concentrate the obtained organic solution by rotary evaporation, and dissolve in ethyl acetate / n-hexane (1 / 3) mixed solution Precipitation gave a white product. Its reaction equation is shown in f...

Embodiment 2

[0042] In this embodiment, a method for preparing a zwitterionic hydrogel capable of recruiting type II collagen is provided, and the steps are as follows:

[0043] (1) Preparation of functionalized sulfobetaine hydrogel

[0044] Dissolve sulfobetaine monomer, active ester monomer, cross-linking agent MBA and photoinitiator I2959 in 15 mL of ultrapure water, and ultrasonically dissolve all components fully. Add the solution to a 24-well plate with 1 mL of liquid per well. Finally, it is cross-linked into a gel under ultraviolet light. Wherein, the chemical structure of the zwitterionic monomer used as the main structure of the hydrogel is shown in formula (II):

[0045]

[0046] (2) Preparation of type II collagen-binding peptide modified sulfobetaine hydrogel

[0047] Soak the functionalized sulfobetaine hydrogel prepared in step 1 in PBS buffer, and change the water every 6 hours until the unreacted monomers are completely removed. Meanwhile, the WYRGRL polypeptide wa...

Embodiment 3

[0050]In this example, the synthesis scheme of the active ester monomer and the preparation of the hydrogel are basically the same as in Example 1 and Example 2, the difference is that the sulfobetaine monomer in Example 2 is replaced by phosphorylcholine Monomer, to prepare a zwitterionic hydrogel with the ability to recruit type II collagen, denoted as pMPC@PEP. Wherein, the chemical structure of the zwitterionic monomer used as the main structure of the hydrogel is shown in formula (III):

[0051]

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PUM

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Abstract

The invention belongs to the field of polymer functional materials, specifically zwitterionic hydrogel capable of recruiting type II collagen and its preparation method and application. The zwitterionic hydrogel realizes the chemistry of type II collagen-binding polypeptide WYRGRL by introducing co-polymerized active ester monomers. Modified, prepared by UV cross-linking. This hydrogel can specifically bind type II collagen in cartilage components. When co-cultured with chondrocytes or bone marrow mesenchymal stem cells, it can preferentially capture a large amount of type II collagen in situ, thereby promoting the process of cartilage differentiation and improving hydraulic coagulation. Gum cartilage repair ability. At the same time, the lubricity provided by the zwitterionic monomer can avoid the wear of the hydrogel during high-shear motion. This zwitterionic hydrogel with II collagen recruitment function has broad application prospects in bone tissue engineering.

Description

technical field [0001] The invention belongs to the field of polymer functional materials, and relates to a zwitterionic hydrogel capable of recruiting type II collagen and its preparation method and application, which can be used to recruit type II collagen secreted by bone marrow mesenchymal stem cells or chondrocytes, and its biological application scene broad. Background technique [0002] The main components of bone joints are type II collagen, hyaluronic acid, lubricating factor, proteoglycan PRG4, chondroitin sulfate, etc. These components are essential for normal joint activities. However, with the occurrence and aggravation of osteoarthritis, these matrices are gradually lost into the joint fluid and metabolized under the influence of the inflammatory environment. Therefore, the treatment of osteoarthritis requires the recruitment of these biomacromolecules into the cartilage components. Among them, type II collagen is mainly secreted by bone marrow mesenchymal ste...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08F220/38C08F230/02C08F220/36C07K7/06A61L27/52A61L27/22C08L33/14C08L43/02
CPCC08J3/075C08F220/387C08F230/02C07K7/06A61L27/52A61L27/227C08J2333/14C08J2343/02C08F220/36
Inventor 俞鹏李建树谢婧郑友超任凯
Owner SICHUAN UNIV
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