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A kind of mesenchymal stem cell membrane camouflage magnetic drug-loaded nanoparticle and its preparation method and application

A nanoparticle, drug-carrying nanotechnology, applied in nanotechnology, pharmaceutical formulations, nanotechnology, etc., can solve problems such as the inability to guarantee the effective functioning of drugs and the limitation of damaged parts.

Active Publication Date: 2022-07-05
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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Problems solved by technology

However, cartilage defect is not the same as extensive cartilage lesions in osteoarthritis. Its main damaged part is relatively limited. Even if the therapeutic drug is injected into the joint cavity, it cannot guarantee that the drug can effectively play a role in the damaged part. Therefore, how to make the drug stable and efficient Transport to the lesion site is the key to effective drug efficacy

Method used

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  • A kind of mesenchymal stem cell membrane camouflage magnetic drug-loaded nanoparticle and its preparation method and application
  • A kind of mesenchymal stem cell membrane camouflage magnetic drug-loaded nanoparticle and its preparation method and application
  • A kind of mesenchymal stem cell membrane camouflage magnetic drug-loaded nanoparticle and its preparation method and application

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preparation example Construction

[0043] The present invention provides a method for preparing the mesenchymal stem cell membrane camouflaged magnetic drug-carrying nanoparticles according to the above technical solution, comprising the following steps:

[0044] The drug-containing solution and Fe 3 O 4 Nanoparticles were co-incubated at 20-30 °C for 22-26 h to obtain drug-loaded Fe 3 O 4 Nanoparticle dispersion; the medicine is selected from the medicines that promote cartilage differentiation and repair;

[0045] Mesenchymal stem cell membrane (BMSC) and drug-loaded Fe 3 O 4 After mixing the nanoparticle dispersions, ultrasonic incubation is performed for coating to obtain mesenchymal stem cell membrane camouflaged magnetic drug-loaded nanoparticles.

[0046] The present invention combines the drug-containing solution and Fe 3 O 4 Nanoparticles were co-incubated at 20-30 °C for 22-26 h to obtain drug-loaded Fe 3 O 4 Nanoparticle dispersion. In the present invention, the co-incubation time is prefer...

Embodiment 1

[0067] Preparation of drug-loaded nanoparticles: take 1.08g FeCl 3 ·6H 2 O, 0.24g NaCit 2H 2 O, 1.20 g of NaAc was dissolved in 20 ml of ethylene glycol, and after ultrasonic dissolution, magnetic stirring was performed for 0.5 h. Transfer to a hydrothermal kettle and react at 200°C for 10 hours. After the reaction, rinse the kettle with cold water to room temperature. The product was washed 3 times with water, 3 times with alcohol, and placed in ethanol for use. Weigh 3mg KGN into a 5ml EP tube, add 100μL DMSO to dissolve, and weigh 15mg Fe 3 O 4 Nanoparticles, mix the two into a 15mL centrifuge tube, make up to 15mL with double distilled water, put them in a rotary mixer and spin for 24h to obtain drug-loaded Fe 3 O 4 Nanoparticles;

[0068]Membrane lift: Stem cells cultured to a confluence of 70%-80% in a petri dish were washed with 1×PBS, digested with 0.05% trypsin-EDTA, terminated, centrifuged at 1000 rpm for 10 min, and the supernatant was discarded. Add 0.25mmo...

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Abstract

The invention provides a mesenchymal stem cell membrane camouflaged magnetic drug-carrying nanoparticle and a preparation method and application thereof. The drug-carrying nanoparticle comprises drug-carrying Fe 3 O 4 Nanoparticles and composites in drug-loaded Fe 3 O 4 Mesenchymal stem cell membrane on nanoparticle surface; drug-loaded Fe 3 O 4 Drugs and Fe in Nanoparticles 3 O 4 The mass ratio of nanoparticles is 1:4.5 to 5.5; the drug is selected from drugs that promote cartilage differentiation and repair; drug-loaded Fe 3 O 4 The ratio of the mass of nanoparticles to the number of cells derived from the mesenchymal stem cell membrane is (3.4-3.9) mg:5×10 6 indivual. The drug-loaded nanoparticles use the mesenchymal stem cell membrane as a carrier, and will be loaded with Fe-containing drugs that promote cartilage differentiation and repair. 3 O 4 The nanoparticle coating can stably and efficiently transport the drug to the lesion site, so that it can promote the repair of cartilage tissue. It is non-toxic, degradable, and has good biocompatibility; it is better than simple topical medication to promote cartilage repair.

Description

technical field [0001] The invention belongs to the technical field of cell membrane camouflage nanoparticles, in particular to a mesenchymal stem cell membrane camouflage magnetic drug-carrying nanoparticle and a preparation method and application thereof. Background technique [0002] Defects of articular cartilage caused by trauma or overuse may cause joint pain, impair joint range of motion, and advance the progression of osteoarthritis. At present, the treatment of cartilage defects has limitations. The main treatment methods are limited to two methods: microfracture and cartilage transplantation. However, for larger defects, the effect of microfracture treatment may not be large, and it is difficult for patients to quickly restore function. Therefore, , most doctors will choose cartilage transplantation to repair larger defects. [0003] The advantage of cartilage transplantation is that the operation is simple and the risk is low. The main component of the transplant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/02A61K47/46A61K31/192A61P19/04B82Y5/00
CPCA61K9/5115A61K9/5184A61K31/192A61P19/04B82Y5/00
Inventor 陈俊陈世益张兴宇李云霞
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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