The invention relates to environmental-friendly diatom ooze ink. The ink is prepared from, by weight, 10-20 parts of liquid supernatant of the flowers and leaves of Lantana camara L, 3-15 parts of liquid supernatant of the flowers and leaves of Pelargonium hortorum Bailey, 1-10 parts of liquid supernatant of the flowers and leaves of lavender, 5-10 parts of bamboo charcoal powder, 350-450 parts of deionized water, 15-20 parts of antifreezing cosolvent, 1-3 parts of wetting agent, 1-4 parts of dispersing agent, 140-165 parts of carbon black powder, 40-60 parts of tubular diatomite, 30-40 parts of nanometer titanium dioxide, 140-160 parts of emulsion, 10-20 parts of coalescing agent, 1-5 parts of nanometer zinc dioxide and 0.4-0.8 part of thickening agent. According to the ink, pure natural liquid supernatant of the flowers and leaves of Lantana camara L, Pelargonium hortorum Bailey and lavender are used as one of main ingredients, the ink is prepared according to a certain preparation technology, when a picture is drawn or calligraphy is written with the ink, the ink smell not only achieves the effects of preventing and dispelling booklices, but also has the functions of mildew prevention, bacteria prevention, moisture protection, freeze protection, slow drying protection, wetting and the like.