The invention discloses an adherent-
cultured cell activity detection method and device based on impedance
spectroscopy. An interdigital
electrode, which is parallel with the bottom surface of a culture dish, is arranged at the bottom of the interior of the culture dish; two poles of the interdigital
electrode are connected to a controller by virtue of lead wires; a DDS (
direct digital synthesizer) frequency generator, which is controlled by a
single chip microcomputer in the controller, is promoted to transmit sine
waves which are in n pieces of frequencies from
high frequency to
low frequency; a current
excitation signal, after being transformed by a
voltage-controlled
current source, is outputted to the interdigital
electrode; n pieces of response voltages of the interdigital electrode, by virtue of a
differential amplifier, are inputted to a
single chip microcomputer after undergoing analog-to-
digital conversion; the
single chip microcomputer is used for calculating a complex impedance value under different frequencies, and is used for calculating a dual-layer
capacitance value in accordance with an
equivalent circuit model and the complex impedance value; the
capacitance value of a dual-layer
capacitor in an
equivalent circuit serves an index for assessing
cell activity, so that more accurate
cell activity information can be obtained; and the method and the device can be used for monitoring the
cell activity in real time, cannot damage the
steady state of cells and can achieve quantitative measurement on the
cell activity.