The invention provides a target object behavior data acquisition method based on videos, comprising: first, playing a pending video; according to a scheduled target recognition algorithm and a target tracking algorithm, identifying and tracking a target object in the video, and according to the corresponding relation between a video pixel coordinate and a physic coordinate, calculating the physic coordinate of the target object; and finally, calculating the behavior data of the target object according to the physic coordinate, corresponding frames and video frame rates. Compared with the prior art, the method is based on videos, and utilizes a target recognition algorithm, a target tracking algorithm and the corresponding relation between a video pixel coordinate and a physic coordinate, thereby obtaining the physic coordinate of a target object in a video; and then, according to the frame rate and frame number of the video, the method calculates target object behavior data, thereby providing real, effective, objective and vast data support for research of pedestrian microscopic behaviors.