Cell activity detection method and device based on impedance spectroscopy

Abstract

The invention discloses an adherent-cultured cell activity detection method and device based on impedance spectroscopy. An interdigital electrode, which is parallel with the bottom surface of a culture dish, is arranged at the bottom of the interior of the culture dish; two poles of the interdigital electrode are connected to a controller by virtue of lead wires; a DDS (direct digital synthesizer) frequency generator, which is controlled by a single chip microcomputer in the controller, is promoted to transmit sine waves which are in n pieces of frequencies from high frequency to low frequency; a current excitation signal, after being transformed by a voltage-controlled current source, is outputted to the interdigital electrode; n pieces of response voltages of the interdigital electrode, by virtue of a differential amplifier, are inputted to a single chip microcomputer after undergoing analog-to-digital conversion; the single chip microcomputer is used for calculating a complex impedance value under different frequencies, and is used for calculating a dual-layer capacitance value in accordance with an equivalent circuit model and the complex impedance value; the capacitance value of a dual-layer capacitor in an equivalent circuit serves an index for assessing cell activity, so that more accurate cell activity information can be obtained; and the method and the device can be used for monitoring the cell activity in real time, cannot damage the steady state of cells and can achieve quantitative measurement on the cell activity.

Description

technical field [0001] The invention relates to a cell activity monitoring method and device. The cell activity monitoring particularly refers to the real-time on-line monitoring of the activity of adherent cultured cells. Background technique [0002] Cell research is the basis of many disciplines such as medicine, life sciences, and bioengineering, and cell activity detection technology is widely used in drug screening, toxicology tests, immune function evaluation, proteomics, and the activity of food-sourced and drug-sourced ingredients. identification and other fields. As anti-tumor research continues to heat up, cell viability detection also plays an important role in tumor cell sensitivity tests. [0003] In order to meet the detection needs under different conditions, researchers have explored a variety of cell activity detection methods. According to the different detection principles, the commonly used detection methods in the laboratory can be roughly divided int...

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Problems solved by technology

At the same time, labeling also increases the complexity and uncertainty of the experiment. Factors such as the quality of the labeling dye itself, the standardization of labeling operations, and the time of labeling may affect the final test results.

In addition, some marking dyes are expensive and harmful to the environment.

[0006] 2. End point detection: All detection methods are to take samples at a certain node of cell research to detect cell activity. After the detection is completed, the cells used for detection cannot continue to be cultured

These detection methods belong to the category of endpoint detection, and there is no effective means to realize real-time monitoring of cell activity during the growth cycle of cells.

[0007] 3. Dependence on manual operation: traditional detection is inseparable from the manual operation of operators, and operators with different levels of proficiency have certain influence on the results of the same experiment

Therefore, the total impedance cannot directly reflect the tightness of adhesion between cells and electrodes, and it is selected as an indicator for evaluating cell activity. The final detection result will be interfered by many factors such as electrolytes, electrodes, and cell types.

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