34results about How to "Realize tomography" patented technology

The invention relates to a spectral pupil laser confocal Brillouin-Raman spectrum measuring method and device and belongs to the technical field of microscopy spectral imaging. The device comprises a light source system for generating stimulating light beams, a measuring objective lens, a lighting pupil, a collecting pupil, a dichroic light splitting device, a spectroscope, an Raman spectrum detecting device, a Brillouin spectrum detecting device, a spectral pupil laser confocal detecting device, a three-dimensional scanning device, a displacement sensor and a data processing unit. The method and the device provided by the invention has the advantages that the abandoned rayleigh scattering light in confocal Raman spectrum detection is utilized to build a spectral pupil confocal microimaging system to realize the high-resolution imaging of the three-dimensional geometric position of a sample, the basic property and multiple cross effects of a substance are obtained by detecting the abandoned brillouin scattering light in confocal Raman spectrum detection and further the stress, elastic parameters and density of a material can be measured; the advantages of the confocal Raman spectrum detection technology and the confocal Brillouin spectrum detection technology are utilized to complement each other, and the comprehensive measurement and decoupling of multiple property parameters of the material are realized.

A real-time optico-acoustic imaging method based on acoustic lens and polarization detection is disclosed. The acoustic lens is used to generate the acoustic pressure distribution of biologic tissue duto optico-acoustic effect and couple it to an imaging plane for directly imaging on it. A polarization analyzing method is used to convert said acoustic pressure distribution to light intensity distribution, which is recorded by CCD to obtain a clear image of a tissue layer. Its apparatus is composed of transparent flexible rubber layer, optical fiber, acoustic lens, laser, 1 / 4 wave plate, polarization analyzer, CCD, computer, and cylindrical cavity made of aluminum.

A real-time optico-acoustic imaging method based on acoustic lens and reflecting film detection is disclosed. The acoustic lens is used to generate the acoustic pressure distribution of biologic tissue duto optico-acoustic effect and couple it to an imaging plane for directly imaging on it. A multi-layer reflecting film is used to convert said acoustic pressure distribution to light intensity distribution, which is recorded by CCD to obtain a clear image of a tissue layer. Its apparatus is composed of transparent flexible rubber layer, optical fiber, acoustic lens, two lasers, sound-transmission opaque material, multi-layer reflecting film, CCD, computer, and cylindrical cavity made of aluminum.

The invention belongs to the technical fields of optical microscopy imaging and optical precision measurement and relates to a laser differential confocal spectrum microscopy tomography device which mainly comprises a Raman spectrum analysis part (25) and an objective lens (6), a polarizing spectroscope (7), a one-quarter glass slide (8) and a measurement objective lens (9) which are sequentiallyarranged along the optical path; the laser differential confocal spectrum microscopy tomography device further comprises a differential confocal detection part (26) which is positioned in the reversedirection of the reflection direction of the polarizing beam splitter. The differential confocal detection part is used for measuring the geometric position of a micro-area of a sample and focusing the sample to obtain image information of the micro-area of the sample; the Raman spectrum analysis part is used for analyzing the material spectrum of the detected area of the sample to obtain component information of the micro-area of the sample; the combination of the two parts can realize the nano-level micro-area spectrum measurement of the sample and simultaneously obtain the geometric feature and the material component information of the micro-area of the sample. The laser differential confocal spectrum microscopy tomography device provides a powerful observation means for bio-medicine, material science, high-energy physics and other forefront subjects.

The invention belongs to the technical field of micro-spectrum imaging detection, and relates to a reflection-type confocal CARS micro-spectrum test method and a device. According to the reflection-type confocal CARS micro-spectrum test method, fusion of laser confocal microtechnique and CARS spectrum detection technology is realized, a binary beam splitting system is adopted for nondestructive separation of Reyleigh scattering light and CARS light, wherein CARS light is used for spectrum detection, and Reyleigh scattering light is used for geometric positioning. Accurate capturing and positioning of exciting light focal point position is realized based on the corresponding performance of confocal curve top point to focal point, high precision geometric detection and high spatial resolution spectrum detection are realized, and the method and the device capable of realizing sample microdmain high spatial resolution spectrum detection are constructed. The intensity of sample information-loaded Raman scattering light excited via combination of CARS microtechnique is much higher than that of conventional spontaneous Raman light, excitation time is short, and possibility is provided for rapid detection of biological samples and chemical materials. The reflection-type confocal CARS micro-spectrum test method possesses advantages such as accurate positioning, high spatial resolution, high spectrum detection sensitivity, and controllable measuring focusing spot size; and application prospect in the fields such as biomedicine and material detection is promising.

The invention discloses a method and device for adaptively scanning tomographic microimaging with wide view field and high-throughput, and belongs to the technical field of microimaging. According tothe method, the target imaging area of a sample is divided into a plurality of sub-areas by utilizing ultrashort pulse laser through a method of combining spatial and temporal focusing and scanning, each sub-area is quickly and adaptively scanned by adopting a time division multiplexing method, synchronous data collection is carried out, reconstruction and data processing are performed on collected microimages in each sub-area to obtain a three-dimensional spatial message of the target scanning area in a scanning cycle, and the four-dimensional information of a sample (x, y, z and t) is acquired through three-dimensional spatial scanning and delayed scanning. The device comprises an ultrashort pulse laser light source and beam conversion system, a sub-area scanning system combined with thespatial and temporal focusing technology, an optical microscopic and filter system, a synchronous microimaging system and an image reconstruction and data processing system. The device has the advantages of wide view field, high throughput, low exciting power, high signal-to-noise ratio and the like.

The invention discloses a composite nanoparticle for sensitizing tumor radiotherapy and a preparation method and application of the composite nanoparticle. The composite nanoparticle comprises proteinand bismuth selenide and manganese dioxide which grow on the protein. The preparation method comprises the steps that a manganese salt solution is added into a aqueous dispersion of bismuth selenide-protein nanoparticles, after uniform mixing is carried out, a strong alkaline solution is added, the pH value is adjusted to alkaline, a heating temperature control reaction is carried out, ultrafiltration and washing are carried out, and the composite nanoparticle is obtained. The composite nanoparticle has the advantages that the water dispersibility and biocompatibility are good, radiotherapy is sensitized by increasing the local radiation dosage through bismuth selenide and improving tumor hypoxia through manganese dioxide, and computed tomography, magnetic resonance and photoacoustic multimode imaging angiography can be achieved simultaneously; the preparation method of the composite nanoparticle is simple, convenient and practical, the condition is mild, the controllability is good,and the implementation and promotion are easy; the composite nanoparticle can achieve the integration of targeted radiotherapy sensitization, diagnosis and treatment of the tumor, and has a broad application prospect in the fields of nano-medicine, disease diagnosis, tumor treatment and the like.

The invention belongs to the technical field of spectral measurement and relates to a high-space-resolution double-shaft confocal atlas micro-imaging method and device. According to a core concept of the method, a double-shaft confocal micro-technique and a spectral detection technique are organically merged, and a normally-forgotten Reyleigh light is utilized to carry out auxiliary detection, so that the space resolution of a system is improved, and three detection modes including three-dimensional tomographic imaging, spectral detection and microcell atlas tomographic imaging are realized. The method and the device have the advantages of high space resolution, accuracy in positioning, high spectral detection sensitivity and the like, have wide application prospects in the fields of biomedicines, physical material science, petrochemical engineering, environmental science and the like and provide new ways for the high-space-resolution detection of microcell three-dimensional geometric positions and spectra.

The invention provides a scanning method for achieving high-resolution large-view-field CL imaging of plate-shaped samples. The method includes the steps that parameters are input, the number and the mode of spliced subimages are adjusted and determined, data are collected and spliced, and results are output. Projection data are collected through segmented scanning of the plate-shaped samples under the high-amplification-ratio condition, and high-resolution large-view-field faulted images of the large-area plate-shaped samples are finally obtained through the image splicing method in a square field-character-shaped or hexagonal honeycomb-shaped mode.

The invention belongs to the technical field of optical microscopic imaging and spectral measurement, and relates to a method and a device for confocal Raman spectrum detection with high spatial discrimination. According to the method and the device, confocal technique is integrated into the spectrum detection; nondestructive separation is carried out on Rayleigh scattering light and Raman scattering light by using of a dichroic beam splitting system (13); by using the characteristic that the maximum value of a confocal curve (34) corresponds to a focal position accurately, spectral information exciting a light spot focal position is accurately acquired through maximizing; and the spectrum detection with the high spatial discrimination is achieved, and the method and the device which are capable of achieving the sample microcell spectrum detection with the high spatial discrimination are formed. The method and the device for the confocal Raman spectrum detection with the high spatial discrimination have the advantages of being accurate in positioning, high in spatial discrimination, high in spectrum detection sensitivity and the like, and has broad application prospect in the fields of biomedicine, court evidence collection and the like.

The invention belongs to the technical field of microspectrum imaging detection, and relates to a beam-splitting pupil laser confocal CARS (coherent anti-Stokes Raman Scattering) microspectrum test method and device. The core concept of the method and the device is that the beam-splitting pupil laser confocal microtechnique and the CARS spectrum detection technique are fused, a two-directional beam splitting system separates Reyleigh scattering light and CARS light in a lossless manner, wherein the CARS light detects spectrum and the Reyleigh scattering light geometrically locates. The characteristic that the positions of the peak and the focus of a beam-splitting pupil laser confocal curve accurately correspond is utilized, the position of the focus of an excitation light spot is accurately captured and located, the high-precision geometrical detection and the high-spatial discrimination spectrum detection are realized, and the high-spatial discrimination spectrum detection method and the high-spatial discrimination spectrum detection device are formed for a sample in a microcell. By combining the CARS microtechnique, the excited Raman scattering light loaded with sample information is far stronger than the traditional spontaneous Raman light, the excitation time is short, and the rapid detection of a biological sample and a chemical material becomes possible. The method and the device have the advantages of accurate locating, high space discrimination, high spectrum detection sensitivity, and controllable size of a measured focusing spot, and have broad application prospects in the fields of biomedicine, material detection, etc.