A high spatial resolution confocal Raman spectroscopy detection method and device

Abstract

The invention belongs to the technical field of optical microscopic imaging and spectral measurement, and relates to a method and a device for confocal Raman spectrum detection with high spatial discrimination. According to the method and the device, confocal technique is integrated into the spectrum detection; nondestructive separation is carried out on Rayleigh scattering light and Raman scattering light by using of a dichroic beam splitting system (13); by using the characteristic that the maximum value of a confocal curve (34) corresponds to a focal position accurately, spectral information exciting a light spot focal position is accurately acquired through maximizing; and the spectrum detection with the high spatial discrimination is achieved, and the method and the device which are capable of achieving the sample microcell spectrum detection with the high spatial discrimination are formed. The method and the device for the confocal Raman spectrum detection with the high spatial discrimination have the advantages of being accurate in positioning, high in spatial discrimination, high in spectrum detection sensitivity and the like, and has broad application prospect in the fields of biomedicine, court evidence collection and the like.

Description

technical field [0001] The invention belongs to the technical field of optical microscopic imaging and spectral measurement, and relates to a high spatial resolution confocal Raman spectral detection method and device, which can be used for three-dimensional shape reconstruction and micro-region spectral detection of various samples. technical background [0002] Laser confocal Raman spectroscopy testing technology is a new technology that combines microscopic analysis technology and Raman spectroscopy analysis technology. It focuses the incident laser light on the sample through the microscope, so that it can obtain The molecular structure and so on of the illuminated sample micro-area are called molecular probes. It can not only observe the Raman spectrum signals of different micro-regions in the same layer of the sample, but also observe the Raman signals of different layers in the sample at different depths, and perform tomographic scanning on the tested sample, so as to...

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Problems solved by technology

[0007] The purpose of the present invention is to propose a confocal Raman spectroscopy detection method and device with high spatial resolution in order to overcome the deficiency that the spatial resolution of the existing confocal Raman spectroscopy detection technology is difficult to improve

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