Method and device for testing spectral pupil laser differential confocal CARS micro-spectrum

Abstract

The invention belongs to the technical field of micro-spectral imaging detection and relates to a method and device for testing a spectral pupil laser differential confocal CARS micro-spectrum. The core thought is as follows: Rayleigh light and CARS light loaded with tested sample spectral characteristics are excited by double lasers as a light source; and nondestructive separation is carried out on the Rayleigh light and the CARS light by using a dichroic optical system, wherein the Rayleigh light is subjected to geometrical detection and positioning, and the CARS light is subjected to spectrum detection. By using the characteristic that a zero crossing point of the spectral pupil laser differential confocal curve accurately corresponds to a focus position, the focus position of an excitation beam is accurately captured and positioned, and high-precision geometrical detection and high-spatial-resolution spectrum detection are achieved to form the method and device capable of achieving high-spatial-resolution spectrum detection of a sample micro-region. Through combining a CARS microscopical technique, the excited Raman scattering light loaded with sample information is shorter than a traditional spontaneous Raman effect in time, and the sample can be quickly subjected to nondestructive detection. The method and the device have the advantages of accurate positioning, high spatial resolution, nondestructive detection and high spectrum detection sensitivity, and a new way is provided for micro-region spectrum detection and geometrical measurement.

Description

technical field [0001] The invention belongs to the technical field of microspectral imaging, and relates to a split-pupil laser differential confocal CARS microspectral testing method and device, which can be used to quickly detect micro-area anti-Stokes scattering (CARS) spectra of various samples, High spatial resolution imaging and detection can be realized. technical background [0002] Optical microscopes are widely used in the fields of biomedicine and material science. With the rapid development of modern science, the requirements for microscopic imaging have shifted from structural imaging to functional imaging. In 1990, the successful application of confocal Raman spectroscopy greatly improved the possibility of exploring the specific composition and morphology of tiny objects. It combines confocal microscopy technology and Raman spectroscopy technology. It has the high-resolution tomographic imaging characteristics of confocal microscopy, and has the ability of n...

AI Technical Summary

Problems solved by technology

[0006] The above reasons limit the ability of the CARS microscope system to detect micro-region spectra, and restrict its application in finer micro-region spectrum testing and analysis occasions

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