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Method and device for adaptively scanning tomographic microimaging with wide view field and high-throughput

A micro-imaging, wide-field-of-view technology, applied in material excitation analysis, fluorescence/phosphorescence, etc., can solve problems such as increasing excitation light power, and achieve the effects of reducing light damage, reducing power level, and high excitation efficiency

Active Publication Date: 2019-01-11
TSINGHUA UNIV
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Problems solved by technology

[0009] The purpose of the present invention is to overcome the deficiencies of the prior art, and propose a method and device for adaptive scanning wide-field high-throughput tomographic microscopy imaging. Challenges of increasing excitation optical power and enabling efficient use of data throughput

Method used

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  • Method and device for adaptively scanning tomographic microimaging with wide view field and high-throughput
  • Method and device for adaptively scanning tomographic microimaging with wide view field and high-throughput
  • Method and device for adaptively scanning tomographic microimaging with wide view field and high-throughput

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Embodiment 1

[0057] Refer below image 3Describe in detail an adaptive scanning wide-field tomographic imaging device according to Embodiment 1 of the present invention. filter system, synchronous microscopic imaging system, and image reconstruction and data processing system 317 , the biological sample is placed on the sample stage 318 . Among them, the ultrashort pulse laser source 301 in the ultrashort pulse laser source and beam conversion system adopts Coherent Chameleon Discovery series femtosecond lasers, and the beam conversion system adopts a Kepler telescope system composed of lens 302 and lens 303 (a 4f system) The subarea scanning system based on spatio-temporal focusing includes grating 304, lens 305, scanning galvanometer 307, scanning galvanometer 310, and a lens group made up of lens 308 and lens 309; optical microscope and filtering system includes lens 311, lens 312 and the optical microscopic system that microscopic objective lens 313 forms, and the filter system that i...

Embodiment 2

[0060] Refer below Figure 4 Describe in detail an adaptive scanning wide-field tomographic imaging device according to Embodiment 2 of the present invention. filter system, synchronous microscopic imaging system, and image reconstruction and data processing system 417 , and biological samples are placed on a sample stage 418 . Among them, the ultrashort pulse laser source and the ultrashort pulse laser source 401 in the beam conversion system adopt Coherent Chameleon Discovery series femtosecond lasers, and the beam conversion system adopts the Kepler telescope system (4f system) composed of lens 402 and lens 403 The sub-area scanning system based on spatio-temporal focusing includes grating 404, lens 405, scanning galvanometer 406, scanning galvanometer 409, and a lens group made up of lens 407 and lens 408; optical microscope and filtering system includes lens 410, lens 411 and the optical microscope system that microscope object lens 413 forms, and the filter system that ...

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Abstract

The invention discloses a method and device for adaptively scanning tomographic microimaging with wide view field and high-throughput, and belongs to the technical field of microimaging. According tothe method, the target imaging area of a sample is divided into a plurality of sub-areas by utilizing ultrashort pulse laser through a method of combining spatial and temporal focusing and scanning, each sub-area is quickly and adaptively scanned by adopting a time division multiplexing method, synchronous data collection is carried out, reconstruction and data processing are performed on collected microimages in each sub-area to obtain a three-dimensional spatial message of the target scanning area in a scanning cycle, and the four-dimensional information of a sample (x, y, z and t) is acquired through three-dimensional spatial scanning and delayed scanning. The device comprises an ultrashort pulse laser light source and beam conversion system, a sub-area scanning system combined with thespatial and temporal focusing technology, an optical microscopic and filter system, a synchronous microimaging system and an image reconstruction and data processing system. The device has the advantages of wide view field, high throughput, low exciting power, high signal-to-noise ratio and the like.

Description

technical field [0001] The invention relates to an adaptive scanning wide-field high-throughput tomographic microscopic imaging method and device, and belongs to the technical field of microscopic imaging. Background technique [0002] Systems biology research, especially the study of biological dynamic processes, usually requires large-scale, high-spatial-resolution observations. For example, in brain science, in order to study the transmission and transformation of nerve impulses in neural networks (such as neural activity across brain regions, etc.); in immunology and oncology, in order to study the transfer or migration of immune cells or tumor cells, etc. , all require large-scale, high-resolution, high-speed, high-throughput in-situ observations in vivo. [0003] However, there is an inherent contradiction between large field of view and high resolution in objective lenses of commercial microscope systems. To this end, several new designs of Mesoscopes have been prop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 孔令杰谢浩张元龙戴琼海
Owner TSINGHUA UNIV
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