Split-pupil laser confocal cars microscopic spectrum testing method and device

Abstract

The invention belongs to the technical field of microspectrum imaging detection, and relates to a beam-splitting pupil laser confocal CARS (coherent anti-Stokes Raman Scattering) microspectrum test method and device. The core concept of the method and the device is that the beam-splitting pupil laser confocal microtechnique and the CARS spectrum detection technique are fused, a two-directional beam splitting system separates Reyleigh scattering light and CARS light in a lossless manner, wherein the CARS light detects spectrum and the Reyleigh scattering light geometrically locates. The characteristic that the positions of the peak and the focus of a beam-splitting pupil laser confocal curve accurately correspond is utilized, the position of the focus of an excitation light spot is accurately captured and located, the high-precision geometrical detection and the high-spatial discrimination spectrum detection are realized, and the high-spatial discrimination spectrum detection method and the high-spatial discrimination spectrum detection device are formed for a sample in a microcell. By combining the CARS microtechnique, the excited Raman scattering light loaded with sample information is far stronger than the traditional spontaneous Raman light, the excitation time is short, and the rapid detection of a biological sample and a chemical material becomes possible. The method and the device have the advantages of accurate locating, high space discrimination, high spectrum detection sensitivity, and controllable size of a measured focusing spot, and have broad application prospects in the fields of biomedicine, material detection, etc.

Description

technical field [0001] The invention belongs to the technical field of microspectral imaging, and relates to a split-pupil laser confocal CARS microspectral testing method and device, which can be used to quickly detect micro-area anti-Stokes scattering (CARS) spectra of various samples, and can realize High spatial resolution geometric imaging and detection can obtain high spatial resolution "map-spectrum integration" images. [0002] technical background [0003] Optical microscopes are widely used in the fields of biomedicine and material science. With the rapid development of modern science, the requirements for microscopic imaging have shifted from structural imaging to functional imaging. In 1990, the successful application of confocal Raman spectroscopy greatly improved the possibility of exploring the specific composition and morphology of tiny objects. It combines confocal microscopy technology and Raman spectroscopy technology. It has the high-resolution tomographi...

AI Technical Summary

Problems solved by technology

[0009] The above reasons limit the ability of the CARS microscope system to detect micro-region spectra, and restrict its application in finer micro-region spectrum testing and analysis occasions

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