Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High spatial resolution dual-axis confocal atlas microscopy imaging method and device

A high spatial resolution, microscopic imaging technology, applied in the field of spectral measurement, can solve the problems of low collection efficiency, unfavorable light source intensity fluctuation suppression, insufficient spatial resolution, etc., to improve measurement accuracy and micro-area Raman spectrum detection capabilities , Improve the effect of horizontal resolution

Active Publication Date: 2015-08-12
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects of low collection efficiency, insufficient spatial resolution, and unfavorable suppression of factors such as ambient background light and light source intensity fluctuations in existing confocal Raman detection instruments, and propose a high spatial resolution Dual-axis confocal spectrum detection method and device

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High spatial resolution dual-axis confocal atlas microscopy imaging method and device
  • High spatial resolution dual-axis confocal atlas microscopy imaging method and device
  • High spatial resolution dual-axis confocal atlas microscopy imaging method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0043] In this embodiment, the light source 1 is a laser, the polarization modulation system 29 is a radially polarized light generator, the dichroic light splitting system 8 is a Notch filter, the data processing system 21 is a computer, and the image acquisition system 11 is a CCD detector. 24 is a CCD detector, and the image magnification system 10 is a magnifying objective lens.

[0044] Such as figure 1 , figure 2 and Figure 5 As shown, the illumination objective lens 2 and the collection objective lens 7 are symmetrically distributed on both sides of the normal line 5 of the measurement surface, and the included angle between the illumination optical axis 4 and the normal line 5 of the measurement surface is θ 1 6. The angle between the collection optical axis 20 and the normal line 5 of the measurement surface is θ 2 31, where θ 1 = θ 2 , a high spatial resolution dual-axis confocal atlas microscopic imaging method, the measurement steps are:

[0045] Firstly, t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of spectral measurement and relates to a high-space-resolution double-shaft confocal atlas micro-imaging method and device. According to a core concept of the method, a double-shaft confocal micro-technique and a spectral detection technique are organically merged, and a normally-forgotten Reyleigh light is utilized to carry out auxiliary detection, so that the space resolution of a system is improved, and three detection modes including three-dimensional tomographic imaging, spectral detection and microcell atlas tomographic imaging are realized. The method and the device have the advantages of high space resolution, accuracy in positioning, high spectral detection sensitivity and the like, have wide application prospects in the fields of biomedicines, physical material science, petrochemical engineering, environmental science and the like and provide new ways for the high-space-resolution detection of microcell three-dimensional geometric positions and spectra.

Description

technical field [0001] The invention belongs to the technical field of spectrum measurement, and relates to a high spatial resolution dual-axis confocal atlas microscopic imaging method and device, which can be used for three-dimensional shape reconstruction and micro-region spectral detection of various samples. technical background [0002] Laser confocal Raman spectroscopy is an extremely important basic method for analyzing the structure and composition of samples. This technology not only inherits the high-resolution tomography features of confocal microscopy, but also can perform spectral analysis on samples, thus making laser confocal Raman spectroscopy unique in the field of spectral testing, and rapidly developing into an extremely important It is an important method for sample structure and composition analysis, making it widely used in frontier basic research in chemistry, biology, medicine, physics, geology, forensic evidence collection, criminal investigation an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
Inventor 王允赵维谦崔晗邱丽荣
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com