The invention discloses a folium isatidis and betel nut cooperated disease-resistant broiler chicken feed and a preparation method thereof. The folium isatidis and betel nut cooperated disease-resistant broiler chicken feed is prepared from, by weight, 500-600 parts of fresh cassava residues, 1.5-2.5 parts of sodium citrate, 32-36 parts of taro leaves, 4-5 parts of folium isatidis, 3-4 parts of betel nuts, 7-9 parts of budworm powder, 26-28 parts of cabbages, 15-17 parts of asparagus, 2-3 parts of corn oil, 23-25 parts of red bean powder, 6-8 parts of lily petal powder, 27-29 parts of lemon grass, 21-25 parts of Japanese banana leaves, 64-67 parts of palm meal, 43-46 parts of soy sauce residues, 7-9 parts of Pyrus ussuriensis juice, 54-58 parts of corn residues, 24-26 parts of black bean residues, 21-24 parts of rice wine, 3-4 parts of rose petal jam and 0.2-0.3 part of lentinan. The feed can defend the infraction of escherichia coli, staphylococcus aureus, influenza viruses on chicken bodies, decrease enteritis, bronchitis, viral hepatitis and other diseases and guarantee the health of chicken flocks through coordination between the folium isatidis and the betel nuts.