The invention discloses a preparation method of human adipose-derived MSCs (mesenchymal stem cells) and an application of the human adipose-derived MSCs in preparation of
medicine for treating diseases. The preparation method of the human adipose-derived MSCs comprises steps as follows:
adipose tissue collection,
adipose tissue transportation,
adipose tissue connection, adipose tissue microblock preparation, SVF (
stromal vascular fraction) extraction,
primary culture, subculturing,
cell cryopreservation,
cell bank establishment and
cell recovery. The human adipose-derived MSCs have a powerful
immunity regulation function and can be applied to preparation of
medicine for treating autoimmune diseases, and compared with
conventional medicine, the human adipose-derived MSCs have the advantages as follows: 1, the treatment is convenient, the
curative effect is lasting, the variety and the number of
medicine for treatment and toxic and side effects caused by medicine treatment can be reduced, even the medicine such as immunosuppressant and the like for treatment is out of service, the death rate and the disability rate are reduced, and
life quality is improved; 2, the safety is good, and toxic and side effects are avoided; 3, the source of adipose tissue is wide, the collection is convenient, fundamental research of the human adipose-derived MSCs is
solid, the preparation technology is mature, the popularization degree is high, and the technology is controllable; and 4, the preparation cost of the human adipose-derived MSCs is lower than that of other biological preparation and can be accepted by most patients.