The invention discloses a method for extracting total flavonoids in grape seeds in a double-aqueous phase
system, belonging to the field of separation and
extraction methods of
natural product active ingredients. The method comprises the following steps of: firstly preparing
grape seed rough extract from grape seeds with
ethanol; then adding absolute ethyl
alcohol,
ammonia sulfate, inorganic salt and water in the
grape seed rough extract to form the double-aqueous phase
system, mixing uniformly, standing for 5-30 minutes at
room temperature for
delamination to obtain an upper phase and a lower phase, concentrating the upper phase through
pressure reduction and
drying so as to obtain total flavonoids of
grape seed. In the double-aqueous phase
system, the
mass percents of grape seed rough extract, absolute ethyl
alcohol,
ammonia sulfate and inorganic salt are respectively 5% to 30%, 10% to 30%, 16% to 30%, and 0 to 5%, the balance is supplemented to be 100% with water, and the pH of the double-aqueous phase system is 3.0 to 11.0. Flavonoids are extracted by utilizing double aqueous phases in the method; the method is simple, the operation is convenient, the production cost is low and the treating capacity is large, small
test data can be directly amplified, and the yield of flavonoids in the grape seeds reaches up to 98.2%; and the method can be operated continuously, and is suitable for industrialized production of flavonoids in the grape seeds.