Method for extracting total flavonoids in grape seeds in double-aqueous phase system

Abstract

The invention discloses a method for extracting total flavonoids in grape seeds in a double-aqueous phase system, belonging to the field of separation and extraction methods of natural product active ingredients. The method comprises the following steps of: firstly preparing grape seed rough extract from grape seeds with ethanol; then adding absolute ethyl alcohol, ammonia sulfate, inorganic salt and water in the grape seed rough extract to form the double-aqueous phase system, mixing uniformly, standing for 5-30 minutes at room temperature for delamination to obtain an upper phase and a lower phase, concentrating the upper phase through pressure reduction and drying so as to obtain total flavonoids of grape seed. In the double-aqueous phase system, the mass percents of grape seed rough extract, absolute ethyl alcohol, ammonia sulfate and inorganic salt are respectively 5% to 30%, 10% to 30%, 16% to 30%, and 0 to 5%, the balance is supplemented to be 100% with water, and the pH of the double-aqueous phase system is 3.0 to 11.0. Flavonoids are extracted by utilizing double aqueous phases in the method; the method is simple, the operation is convenient, the production cost is low and the treating capacity is large, small test data can be directly amplified, and the yield of flavonoids in the grape seeds reaches up to 98.2%; and the method can be operated continuously, and is suitable for industrialized production of flavonoids in the grape seeds.

Description

technical field [0001] The invention belongs to the field of separation and extraction methods for effective components of natural products, and in particular relates to a method for extracting total flavonoids in grape seeds by a two-phase system. technical background [0002] The Hexi Corridor is located between 36° and 40° north latitude. It has the best light, heat, water, soil and other resources for producing high-quality wine grapes. It is one of the best producing areas for organic wine in my country and even in the world. In the wine processing process, a large amount of pomace waste, which accounts for 20-30% of the grape processing volume, is produced every year, mainly including grape skins, grape seeds and fruit stems. Grape seeds contain a variety of active ingredients, mainly flavonoids, proteins, polysaccharides, and proanthocyanidins. The flavonoids in grape seeds have the functions of dilating blood vessels, increasing peripheral blood circulation and bloo...

AI Technical Summary

Problems solved by technology

In addition, in the prior art, the extraction of total flavonoids in grape seeds is generally performed by solvent extraction, and no one has used a two-phase system to extract total flavonoids in grape seeds.

Although the Chinese invention patent CN101125841A discloses a method for extracting grape seed flavonoids, which is extracted multiple times by solvent extraction, one extraction can be completed in 3-5 days, and the yield is 64%, but the extraction of grape seed by solvent extraction In total flavonoids, the process cycle is long, the extraction rate is not ideal, and it is not conducive to industrial production

Images