The invention discloses a method for measuring the total iron content in fine iron powder in iron and steel enterprises. The method comprises the following steps: selecting cobalt sesquioxide as an internal standard substance, weighing 0.6000 + / -0.0002 g of fine iron powder sample, 6.0000 + / -0.0002 g of anhydrous lithium tetraborate and lithium metaborate mixed flux with the mass percentage of 67: 33, 0.03 g of ammonium iodide and 1.0000 + / -0.0002 g of Co2O3, putting into a platinum-platinum crucible, then putting the platinum-platinum crucible into a sample melting machine with the temperature of 1050 DEG C, and pre-standing for 120 seconds, swinging for 900s; and post-standing for 30 seconds, and taking out the sample sheet after melting. According to the method, the problems that in the actual determination process of the total iron in the fine iron powder, the effect of a manufactured sample calibration curve is poor, and points are relatively dispersed can be solved, the accuracy of an analysis result of the X-ray fluorescence spectrometry is improved, and the rapid and accurate determination of the total iron content in the fine iron powder is realized.