67results about How to "Improve selective recognition" patented technology

The invention discloses a reticular ion imprinting polymer for selectively identifying lead ions, and a preparation method and an applicationthereof, and belongs to the field of new materials. The reticular ion imprinting polymer is characterized in that 8-hydroxyquinoline-grafted gelatinpolypeptidewhich is used as a function monomeris synthesized by Mannich reaction, and the advantages of richer activity groups, good shrinkage / swelling property, good filming property and the like are realized; on the basis, a preparation technique for preparing the ion imprinting polymer by a physical filming-secondary swelling-chemical crosslinking three-step preparation type is proposed; by controlling the size of a dish used during swelling, the self torsion / folding of a film material is performed in a particular slit space, so as to obtain various shapes of imprinting polymers; finally, after double crosslinking of genipin / transglutaminase, the reticular lead ion imprinting polymer thin film is formed. Since the function monomer contains multiple types of activity groups, the double-crosslinking system and the particular reticular structure, theion imprinting polymerhas the quick adsorption dynamics, high adsorption capacity and selective identifying function on the lead ions.

A pentacene fluorescence ratio probe and its preparation method and application. The structural formula of the pentacene fluorescence ratio probe is shown in FIG. 3 . The novel pentacene fluorescent ratio probe designed and synthesized by the present invention fully utilizes the D-π-A structure and the characteristics of the fluorescent ratio probe, thereby avoiding the detection process due to its own main body and detection solvent. The result is interference. With this design, the probe showed excellent selectivity for methanol, demonstrating the initial application value of this probe in the food field.

The invention discloses a paper base dual-imprinting material capable of selectively recognizing a protein as well as a preparation method and an application of the paper base dual-imprinting material. The preparation method comprises the steps of immobilizing a graphene oxide-hemin compound on the surface of a filter paper material and preparing the dual-imprinting material on the surface of filter paper. The paper base dual-imprinting material is used for selectively recognizing and quantitatively detecting the protein. The paper base dual-imprinting material has the characteristics of low cost, environmental protection, simplicity in operation, high recyclability, and specific recognition of the protein, and particularly has the excellent characteristics of color development, water retention and hydrophily, and can rapidly detect the protein macromolecule at high throughput.

The invention provides a labeling method for immunological detection of nanometer colloidal gold. The covalent labeling method comprises the following steps: preparing a gold nanoparticle with a small particle diameter through a sodium citrate reduction method, using the prepared gold nanoparticle with the small particle diameter as seed gold, and slowly and dropwise adding chloroauric acid and a weak reducing agent at a constant speed so as to prepare a colloidal gold particle with a large particle diameter; treating an antibody to be labeled or protein with a Traut's reagent so as to combine the treated antibody or the treated protein with the prepared colloidal gold in a coupling manner; or, using the prepared gold nanoparticle with the small particle diameter as the seed gold, slowly and dropwise adding the chloroauric acid and a carboxylic acid reducing agent containing sulfhydryl at a constant speed so as to prepare a colloidal gold particle with the large particle diameter and the sulfhydryl on the surface, activating the prepared colloidal gold particle with a cross-linking agent so as to obtain a functional colloidal gold particle, and combining the treated antibody or the treated protein with the prepared colloidal gold particle in the coupling manner so as to form a gold labelled antibody protein compound. Through the use of the covalent labeling method disclosed by the invention, the stability of the gold labelled antibody protein compound in a complex medium is improved, and the selective recognition to objective protein is greatly enhanced, so that the sensitivity of a gold labelled test paper strip is relatively improved.