34results about How to "Simple and convenient detection operation" patented technology

The invention relates human C-reactive protein (CRP) colloidal gold immunochromatographic assay quantitative test paper, which comprises a sample pad, a bonding pad, a nitrocellulose membrane and absorbent paper mutually lapped on a bottom plate with an adhesive in turn. A colloidal gold-labeled CRP antibody complex is coated on the bonding pad; and a detection line and a quality control line are bonded on the nitrocellulose membrane, and are antibody-enveloped lines. During detection, the samples to be detected contact the lower end of the test paper and are subjected to chromatography along the test paper; if the quality control line and a quantitative line do not develop, the test is ineffective; if only the quality control line develops, the CRP concentration is lower than the lowest detection concentration; and if both the quality control line and the quantitative line become red, the content of the CRP is read through a quantitative detection device. The test paper is characterized in that the quality control line and the quantitative line are respectively arranged from the lower end to the upper end of the test paper along the sample chromatography direction.

The invention discloses a method for synthesis of a benzimidazole derivative phosphate anion fluorescence probe and an application method thereof, and relates to the field of synthesis and application of the fluorescence probe. The synthesis of the benzimidazole derivative phosphate anion fluorescence probe can solve the problem that the florescence probes used for detecting PO43- are very scarce in kinds, that the identification of PO43- can be easily influenced by H2PO4- and HPO42-, and that the florescence probes can not directly carry out identification independently. The Benzimidazole derivative phosphate anion fluorescence probe is 1,4 phenyl double (carbamoyl methyl) N e alkylbenzimidazole ammonium chloride], which is obtained under quaterisation between Dichloro acetyl para-phenylene diamine and N-E alkyl benzene and imidazole. The fluorescence probe dissolves in HEPES buffered solution prepared by deionized water, and then test change in the absorbance value and fluorescence intensity to adjust the existence of PO43-. The Benzimidazole derivative phosphate anion fluorescence probe can be used to directly detect the po43- in water and blood.

The invention discloses a jacket-type oil casing air tightness detection device and a detection method. The jacket-type oil casing air tightness detection device comprises an upper end cover, an upper central pipe, an adjusting joint, a lower central pipe and a lower end cover. Through holes are formed in central positions of the upper central pipe and the lower central pipe and are connected through the adjusting joint. One side of the adjusting joint is provided with a first high-pressure soft pipe joint. The inner walls, provided with the through holes, of the upper central pipe and the lower central pipe and are provided with grooves, and glue cylinders and extruding mechanism are respectively arranged in the grooves. Each extruding mechanism comprises four extruding forks uniformly arranged on the periphery of the glue cylinder, one end of each extruding fork is in contact with the glue cylinder, the other end of the extruding fork extends out a side hole of the upper central pipe or the lower central pipe, and the extending-out part is sleeved by a spring. The tail part of the extending out par of each extruding fork is provided with a piston disk, a piston sleeve sleeves the outer part of the piston disk, the piston disk is connected with the piston sleeve in sliding sealing manner, and the end part of the piston sleeve is provided with a second high-pressure soft pipe joint. The jacket-type oil casing air tightness detection device and the detection method can effectively realize air tightness detection of a small-size oil casing, and the service lifetime of the glue cylinder is effectively prolonged.

The invention discloses a device for detecting the air impermeability of an oil casing screw thread and a detecting method thereof. The device comprises a main clamping body, a left fan-shaped clamping body in rotatable and sealed connection to a side of the main clamping body, and a right fan-shaped clamping body in rotatable and sealed connection to the other side of the main clamping body. The left fan-shaped clamping body and the left fan-shaped clamping body are fixedly connected through a buckle switch. The inner wall of the detecting device is provided with a sealing elastic layer, and a gap between the left fan-shaped clamping body and the left fan-shaped clamping body is in sealed contact by means of a sealing adhesive tape. The main clamping body is provided with an air impermeability detecting device and a hydraulic clamping device for clamping the left fan-shaped clamping body and the left fan-shaped clamping body so that the inner wall of the detecting device and an oil casing are clamped. The detecting device and the detecting method are simple in operation and light in structure, solve the air impermeability detection of the oil casings with different sizes, and can be suspended from an oil derrick for easy field operation and remote control.

The invention discloses an edible oil detecting device, which comprises a bracket, a motor arranged on the bracket, an oil containing box, a temperature controlled heating device, an oil fume sensor, a grease density sensor, a conductivity sensor, and a PLC (Programmable Logic Controller) with a display, wherein the oil containing box is connected to a rotary shaft of the motor cooperatively, theoil containing box is in a funnel shape, the center of the oil containing box is lower than the edge of the box, and the mouth edge of the oil containing box is provided with a grease outlet hole andis matched with a box cover; the temperature controlled heating device is connected to the bracket in a positioning mode and is positioned under the oil containing box; the oil fume sensor is arranged on the box cover of the oil containing box; and the grease density sensor and the conductivity sensor are both arranged on the bracket, and the sensing heads of the grease density sensor and the conductivity sensor face the grease outlet hole. Used oil products can be effectively detected by the device, and the quality of other oil products can also be detected; and the device has high sensitivity and high precision, is easy to operate in detection and is not limited by sites.

The invention relates to establishment of a relay type fluorescence detection of Hg<2+> and S<2-> and aims to solve the problems that very few fluorescence probes are used for relay type fluorescencedetection of Hg<2+> and S<2-> in an aqueous solution in the prior art and recognition of Hg<2+> is easily affected by Au<3+>, Fe<2+> and Fe<3+>. The probe 1,4-o-phenylenediamine-3-(1-amyl benzimidazole)acetanilide chloride [L] can form a stable complex with Hg<2+> in an HEPES aqueous solution, so that fluorescence quenching of L is realized, and the pH value is in the range of 2.0-11.3. The probecan be used for detecting Hg<2+> and has stable fluorescence property; the detection limit of Hg<2+> can reach 4.0*10<-7> mol / L. the compound [L-Hg<2+>] can realize fluorescence detection of S<2->. Therefore, the probe can realize relay type fluorescence detection of Hg<2+> and S<2-> in the aqueous solution and has huge application prospects in the fields of environmental science, analytical chemistry, bioscience and the like.

The invention relates to a rheid leakage testing device and is characterized by comprising a case, a test-piece lock mechanism and an air-pressure balancing and testing system; the front panel of the case is provided with a power switch, a manual valve for selecting, an aeration timer and a test control element; the test-piece fastening mechanism comprises at least two groups of lock sleeve, cone plug, aeration cone tube, lock cylinder and electromagnetic valve; the air-pressure balancing and testing system comprises an observation tube arranged on the case, an air source pressure regulating valve arranged on one side of the case, a standard air bottle in the case, an air bag aerating tube communicated with the standard air bottle and the observation tube, and a test-side aerating tube connected with the observation tube. The invention has the advantages that whether the test-piece has leakage is tested by observing the change of air pressure inside the standard air bottle and the test-piece and whether bubbles are generated in the water in the observation tube, the automation degree is high, the testing operation is simple and quick, the test precision is reliable, the testing quality of the test-piece is improved, the test-piece is prevented from being contaminated in the testing process, and the product quality is ensured.

The invention provides a nanorod-based fluorescence enhancement structure, a nanorod-based fluorescence detection system and an autoinjection detection chip. The fluorescence enhancement structure comprises a substrate, a nanorod and an antibody or single-stranded DNA for modifying the nanorod, wherein the nanorod structure is prepared through tilted angle deposition on a substrate. The autoinjection detection chip has the advantages of realization of highly-sensitive, rapid and microfluidic autoinjection detection of a biomarker, simplicity in operation, and low cost.

The invention provides a real-time fluorescence detection method for detecting various pathogens and application. Specifically, the invention provides a novel simple real-time fluorescence detection (RT-) Polymerase Chain Reaction (PCR) method, a reaction system and application of the reaction system in single and multiple detections of the pathogens (in particular viruses) and quantitative detection of gene expression. The tail end 3' is provided with a fluorescence or quenching group-modified fluorescence primer, so that the end 3' is not required to be mispaired with a template; under the action of a 3'-5' excision enzyme correction function of high-fidelity DNA polymerase and any trace non-high-fidelity DNA polymerase, the fluorescence or quenching group at the end 3 is cut off, so that real-time fluorescence detection of pathogen nucleic acids and DNA and mRNA of human genes is realized. The real-time fluorescence detection method is simple in design, quick in operation, high in sensitivity and high in accuracy, and can be widely applied to relevant fields of quick and multiple detection of infectious pathogens, the quantitative detection of the gene expression and the like.

The invention provides a hanging wheel testing device, and the problem that an existing hanging wheel detecting process is cumbersome is solved. The technical solution point is that the hanging wheeltesting device comprises a rack, a test door sliding in the rack and a power mechanism which drives the test door to slide. The rack comprises upper and lower beams parallel to each other, the upper beam is equipped with a hanging rail for nesting a hanging wheel to be tested at the top of the test door and allowing the hanging wheel to be tested to slide inside, and the test door is provided witha pressure sensor for detecting push-pull force borne by the test door. By installing a wheel to be hung on the top of the test door, the hanging wheel is embedded into the hanging rail installed atthe top of the rack, therefore, a host instructs the power mechanism to drive the test door to move, the pressure sensor on the test door detects the push-pull force during the movement process of thetest door, the host collects the push-pull force detected by the pressure sensor in real time and compares the change of the push-pull force to judge the flatness and service life of the hanging wheel, and the operation is simple and convenient.

The invention discloses a tool and a method for inspecting a size of a jointed board in a sleeper steel mold. The tool comprises a horizontal transverse board, side bumps (1) arranged at the lower ends of the left side and the right side of the horizontal transverse board, and a middle bump (2) in the middle, wherein the planarity of measurement surfaces of the side bumps (1) and the measurement surface of the middle bump (2) is smaller than 0.05; the parallelism between the measurement surfaces of the side bumps (1) and the measurement surface of the middle bump (2) is smaller than 0.1; and the height difference (h+1) mm between the side bumps (1) and the middle bump (2) is selected according to the design size of the steel mold. With the adoption of the tool and the method, the measurement process of the sleeper steel mold can be simplified, and the measurement accuracy is improved.

The disclosure relates to a circuit detection method and system, and aims to solve the problems that an existing circuit detection process is complex in operations and low in accuracy; the circuit detection system comprises a controller, a machinery arm, a testing device and a placement table; the controller is respectively coupled to the machinery arm and the testing device; the testing device is coupled to the machinery arm; the placement table is used for placing the tested circuit; the controller is used for controlling a machinery arm probe to make contacts with the tested circuit for detection; the testing device is used for obtaining the measuring result information through the machinery arm probe, and sending the measuring result information to the controller.

The invention relates to a leakage test device for a medical filter. The leakage test device for the medical filter is characterized by comprising a frame, a water tank fixed inside the frame and an electric control cabinet arranged at one end of the frame, wherein the upper part of the water tank, corresponding to the frame, is provided with a filter submersible cylinder through an installation frame of the submersible cylinder, and is mounted with a compact cylinder which can be moved up and down and a sealing cylinder which can be horizontally moved through an installation frame of the sealing cylinder. The leakage test device for the medical filter has the following advantages: not only can the symptoms such as red and swelling hands, arthralgia and the like and the damage to human health caused by the fact that the hands of an operator are soaked in water for a long time be prevented, but also the automation degree is high, the detection operation is simple and rapid, the detection accuracy is reliable, the detection quality of a tested unit is improved, and the detection efficiency is greatly improved.

The invention discloses a seal test device for a metal housing of a photoelectronic device. The seal test device comprises a base, a seal detection box, a controller, a placement frame, a metal housing body, an air pressure sensor and a vacuum pump; the seal detection box is mounted at the upper part of the base; a controller is mounted on the outer wall of the seal detection box; the placement frame is placed inside the seal detection box; the metal housing body is placed on the placement frame; the air pressure sensor is arranged inside the metal housing body; a box cover is mounted at the upper part of the seal detection box; an exhaust tube is mounted on the seal detection box; the vacuum pump is mounted on the exhaust tube; an air inlet pipe is mounted on the box wall of the seal detection box; and an electromagnetic valve is mounted on the air inlet pipe. By the arranged base, seal detection box, controller, placement frame, metal housing body, air pressure sensor and vacuum pump, the seal test device disclosed by the invention solves problems of low detection speed and low efficiency when the metal housings of the photoelectronic devices are manually detected one by one.

The invention relates to a detection method for sodium acetate in sewage. The detection method comprises the following steps: 1) selecting instruments: an ion chromatograph; a chromatographic column AS19 4mm and a guard column AG19 4mm; a suppressor ASRS; an automatic sampler AS-DV; wherein the ion chromatograph includes a high-pressure infusion pump, an automatic generator of KOH leacheate and anelectrical conductivity detector; 2) selecting reagents: (1) fluorinion in concentration of 500mg / L, (2) chloridion in concentration of 500mg / L, (3) sulfate radical in concentration of 500mg / L, (4) sodium acetate and (5) sodium formate; 3) setting instrument parameters: the conveying flow rate of a high-pressure infusion pump: 1ml / min, KOH leacheate concentration of the automatic generator of KOHleacheate, suppressor current: 20mA, and sampling volume of an automatic sampler: 25muL; 4) drawing a working curve; and 5) determining a sample. The detection method for sodium acetate in sewage hassimple and convenient detection operation, high measuring precision and a great guiding significance in operations of a sewage treatment plant for adding and monitoring sodium acetate in sewage.

The invention discloses a detection machine for the magnetic induction distance and high-low resistance of a brake oil tank sensing module, and the detection machine comprises a rack, an electric control box, a touch display screen, a product conveying mechanism, a magnetic induction distance detection mechanism, a high-low resistance detection mechanism, a high-low resistance unqualified laying-off mechanism, an industrial marking machine, and a qualified product laying-off mechanism. The touch display screen pre-stores a magnetic induction distance standard parameter value, and the magnetic induction distance detection mechanism, the high-low resistance detection mechanism, the high-low resistance unqualified laying-off mechanism, the industrial marking machine and the qualified product laying-off mechanism are sequentially set at an outer side of the product conveying mechanism anticlockwise. The high-low resistance detection mechanism and the high-low resistance unqualified laying-off mechanism are in signal connection. The magnetic induction distance and the high-low resistance are integrated for automatic detection. The detection machine is high in detection efficiency, is low in labor intensity of a worker, saves the manpower cost, is high in detection precision, is not liable to cause detection errors and wrong detection, does not need to carry out the frequent rework and detection, is simple and convenient in detection operation, and cannot cause unnecessary hidden troubles.

The invention discloses a method for rapidly detecting florfenicol and florfenicol amine in honey. The method comprises the following steps: S1, preparing a standard solution; s2, carrying out sample pretreatment; s3, carrying out experiment result and analysis; according to the method, a sample is extracted through ammoniated acetonitrile, the sample is purified through a QuEChERs method, the content of florfenicol and florfenicol amine in honey is measured at the same time through liquid chromatography-triple quadrupole tandem mass spectrometry, and quantification is conducted through an internal standard method. The result shows that the linear relation of florfenicol and florfenicol amine is good in the experimental concentration range, the detection limit ranges from 0.05 microgram / kg to 0.2 microgram / kg, the adding standard recovery rate ranges from 96.5% to 105%, and the relative standard deviation (RSD) ranges from 3.8% to 5.3%. According to the conclusion, the method is simple, convenient and rapid in detection operation, and accurate qualitative and quantitative analysis can be carried out on trace florfenicol and florfenicol amine in honey.

The invention provides a mismatched fluorescence tolerance real-time quantitative PCR method for detecting multiple pathogens and an application in virus (particularly RNA virus with high variability)detection. The invention relates to a DNA polymerase (non-high fidelity DNA polymerase) without a 3'->5'exonuclease correction function and a high fidelity DNA polymerase with the 3'->5'exonuclease correction function, a primer is driven to extend under the action of the non-high fidelity DNA polymerase when no mismatch is caused to form a DNA product. When the 3'end of the primer is mismatched with the template, the high-fidelity DNA polymerase excises the mismatched base at the 3'end of the primer, and a common DNA polymerase is responsible for catalyzing DNA synthesis; the method has the advantages of good specificity, high sensitivity, good repeatability, simple operation, convenience and rapidness, and can be widely applied to the related fields of virus detection and the like.

The invention discloses a detection tool for an ATC system. When voltage values of various interface point locations of a connecting device are measured, an external connector of the tool is in matched connection with the connecting device, various connecting noodles of the external connector are correspondingly inserted into the interface point locations of the connector, and measurement circuitscorresponding to the interface point locations one to one can be formed. Switches on the measurement circuits are turned on / off in sequence, the measurement circuits are switched on in sequence, andthen the voltages of the interface point locations are measured through voltmeters in sequence. According to the detection tool for the ATC system, through automatic matching and reliable connection of the external connector and the connecting device, errors caused by human factors in the detection process are greatly avoided, and the detection reliability is improved. The electric shock risk existing in the detection process is avoided, and the test safety factor is improved. Besides, in the way of adding the switches to the measurement circuits, it is convenient for a tester to carry out anexperiment, the detection operation is simple and convenient, and the detection efficiency is improved.

The invention discloses a near infrared spectrum based water-injected meat detecting method, and belongs to the technical field of food detection. The detecting method comprises the following steps: acquiring infrared spectrum of a sample, obtaining a relative moisture content of the sample through measurement and calculation, then preprocessing the spectroscopic data, obtaining a target matrix, dividing the matrix into a calibration set and a prediction set, in accordance with samples in the two data sets, the corresponding spectroscopic data and the obtained relative moisture content through calculation, establishing a regression prediction model, evaluating the prediction accuracy of the model, repeating the steps for many times, at last determining the optimal preprocessing method and the best prediction model. The equipment for executing the detecting method is simple in structure; the detecting operation is simple and direct, and the detecting process is quick; the method is nondestructive, the detected sample is not required to be specially processed, and an operator is not required to understand specialized knowledge; the near infrared equipment and the assorted device are high in cost performance, suitable for being carried because of the small size, and suitable for popularization in the market; the method can be used for meat finished product markets and further for industrial production lines.