The invention discloses a totally-enclosed
nucleic acid extraction and detection integrated kit, which is characterized in that a plurality of areas are arranged in one kit, and the kit comprises a shell, a cleaning solution
injector, an eluent
injector and a PCR (
Polymerase Chain Reaction) tube which are respectively filled with a
lysis solution, a cleaning solution, an eluent and a reaction solution; when a sample is detected, the external push rod is pushed, a
lysis solution mixed with the sample passes through the
silicon material membrane,
nucleic acid released by
lysis of the sample is adsorbed by the
silicon material membrane, along with pushing of the external push rod, the cleaning solution and the eluent are sequentially extruded out, and
nucleic acid purification and
elution arecompleted on the
silicon material membrane. During
elution, the shell integrally moves downwards with the help of external auxiliary equipment, the needle head penetrates into the closed PCR tube, and the external push rod is pushed to extrude purified nucleic acid to be mixed with reaction liquid. The PCR tube is subjected to
temperature control and nucleic acid amplification reaction through the temperature circulating device, the optical detection equipment detects the amplification result, and totally-enclosed nucleic acid extraction detection is completed.