Totally-enclosed nucleic acid extraction and detection integrated kit

Abstract

The invention discloses a totally-enclosed nucleic acid extraction and detection integrated kit, which is characterized in that a plurality of areas are arranged in one kit, and the kit comprises a shell, a cleaning solution injector, an eluent injector and a PCR (Polymerase Chain Reaction) tube which are respectively filled with a lysis solution, a cleaning solution, an eluent and a reaction solution; when a sample is detected, the external push rod is pushed, a lysis solution mixed with the sample passes through the silicon material membrane, nucleic acid released by lysis of the sample is adsorbed by the silicon material membrane, along with pushing of the external push rod, the cleaning solution and the eluent are sequentially extruded out, and nucleic acid purification and elution arecompleted on the silicon material membrane. During elution, the shell integrally moves downwards with the help of external auxiliary equipment, the needle head penetrates into the closed PCR tube, and the external push rod is pushed to extrude purified nucleic acid to be mixed with reaction liquid. The PCR tube is subjected to temperature control and nucleic acid amplification reaction through the temperature circulating device, the optical detection equipment detects the amplification result, and totally-enclosed nucleic acid extraction detection is completed.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and in particular relates to a fully enclosed nucleic acid extraction and detection integrated kit. Background technique [0002] Genetic testing is a technology for detecting DNA through blood, other body fluids, or cells. It is to take the peripheral venous blood or other tissue cells of the tested person, amplify their genetic information, and use specific equipment to detect the DNA molecules in the tested person's cells. It is a method to detect and analyze the gene types and gene defects contained in it and whether its expression function is normal, so that people can understand their own genetic information, clarify the cause of disease or predict the risk of a certain disease in the body. [0003] Genetic testing can also be used for virus detection, and virus infection can be judged by detecting the presence or absence of viral nucleic acid. Viruses can be divided into DNA viruse...

AI Technical Summary

Problems solved by technology

[0004] At present, the genetic testing procedures are complex and require professionals to conduct testing in the laboratory, which is not conducive to the popularization of genetic testing technology

During genetic testing, samples need to be lysed and washed to obtain nucleic acids in the samples, and then detected by optical equipment after gene amplification. The whole process is complicated, prone to pollution, and takes a long time.

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