The invention relates to an all-male
pelodiscus sinensis fry breeding technology, in particular to a short-period efficient all-male
pelodiscus sinensis fry breeding method. The method comprises the steps of
pelodiscus sinensis egg
temperature control hatching, pelodiscus sinensis egg
estrogen treatment, young pelodiscus sinensis
greenhouse breeding, pseudo-female pelodiscus sinensis screening andparent pelodiscus sinensis pond breeding, wherein the pelodiscus sinensis egg
hatching temperature is controlled to be 28+ / -1 DEG C in the pelodiscus sinensis egg
temperature control hatching; the pelodiscus sinensis egg
estrogen treatment is that an estradiol solution is dropwise added to the
eggshell surface closest to the
embryo position in each of 15-18 time periods of pelodiscus sinensis
embryo hatching; and a fluorescent quantitative PCR technology is adopted in the pseudo-female pelodiscus sinensis screening. According to the method, a sex
hormone dropwise adding and pseudo-female pelodiscus sinensis induction technology is adopted, the operation is easy, the conversion rate is high, the
sexual maturity period is greatly shortened through the young pelodiscus sinensis
greenhouse breeding, and pseudo-female pelodiscus sinensis screened out through the fluorescent quantitative PCR technology are normally mated with original male pelodiscus sinensis to obtain all-male pelodiscus sinensis fries. The all-male pelodiscus sinensis fry breeding method provided by the invention is short in period and high in commercialization degree, and
economic benefits can be remarkably improved.