82results about How to "Facilitate subsequent purification" patented technology

The invention belongs to the technical field of extraction and purification of plant ingredients and particularly relates to a method for extracting and purifying cannabidiol from China-hemp. In the method, an ingredient of cannabidiol is extracted from floral leaves in a mature period of the China-hemp according to a supercritical CO2 extraction technique, a cannabidiol extract is further purified by macroporous adsorbent resin and a rapid purification system, a cannabidiol content of an obtained product is 98% or higher, and the cannabidiol product does not contain a psychotoxic ingredient tetrahydrocannabinol. According to the method, a chemical organic solvent is not used in an extraction process, so that the method is environment-friendly and is low in toxicity and high in efficiency; the loss of cannabidiol in an adsorption elution process is reduced in a purification process, so that the impurity ingredients such as tetrahydrocannabinol and the like are effectively removed, and the use of an organic regent is decreased, so that the purification cost is lowered, and the environmental pollution is reduced. The product is high in yield and purity, and the requirement for development of a value-added product of the China-hemp, in particular to the market requirement of the active ingredient of the cannabidiol product in the China-hemp, can be met, and especially, the cannabidiol product has the great application advantage in the pharmaceutical field.

The invention relates to a method for extracting high-purity steviol glycosides from stevia rebaudiana. The method comprises the following steps: (1) soaking a ground stevia rebaudiana raw material into water, and adding a cellulase preparation for enzymolysis; (2) carrying out heating extraction and centrifugation, and filtering, so as to obtain stevia rebaudiana enzyme extraction liquid; (3) carrying out water-bath heating on the stevia rebaudiana enzyme extraction liquid, adding an inorganic salt flocculating agent, stirring, flocculating, carrying out centrifugation, and filtering, so as to obtain stevia rebaudiana edulcorated liquid; (4) carrying out decolorization adsorption on the stevia rebaudiana edulcorated liquid by virtue of a macroporous adsorption resin column, carrying out elution through eluent, carrying out nanofiltration, and concentrating, so as to obtain a stevia rebaudiana concentrated liquid; and (5) carrying out silica-gel column chromatography and elution on the stevia rebaudiana concentrated liquid, collecting each fraction, concentrating, and drying, so as to obtain multiple high-purity steviol glycosides products. The purity of the products obtained by virtue of the method can reach up to 98.4%, and the final yield is more than 90%; the residual amounts of ethanol and ethyl ester are low, so that the method is environmentally friendly and safe; the method is simple in process, high in operability, low in energy consumption and cost and applicable to industrial production.

The invention discloses a monoclonal antibody resisting CD25 with special variable region of the light chain and variable region of heavy chain structure aiming at special CD25 epitope (IL-2 acceptor). The invention also discloses the coded sequence of the monoclonal antibody, the expression vector and host cell containing the coded sequence and the preparation method thereof. The monoclonal antibody of the invention has excellent immunosuppressive action and low immunogenicity and high specificity.

The invention specifically relates to a method for preparing a fluorine-silicon tri-block copolymer through activators regenerated by electron transfer-atom transfer radical polymerization (ARGET ATRP) under the condition of a low catalyst concentration, belonging to the field of technical materials. According to the invention, an excess weak reducing agent is used to continuously reduce a passivating agent in a system, and an activator prepared through in-situ reaction catalyzes atom transfer radical polymerization of a monomer; in such a process, a small amount of oxygen hard to thoroughly remove in the system can be consumed in situ; the amount of a catalyst used in a polymerization system is substantially reduced at the same time; and the step of removal of copper in subsequent operation can be avoided. The preparation method for the fluorine-silicon tri-block copolymer has the advantages of a low catalyst concentration, mild reaction conditions and suitability for industrial production.

The invention relates to the field of biotechnologies, and particularly discloses a fusion expression and purification method for recombinant proteins by the aid of alkaline tags and intein and application of the fusion expression and purification method. The fusion expression and purification method and the application have the advantages that fusion expression can be carried out on the to-be-studied target proteins, the intein with a self-splicing function and the alkaline tags, and accordingly the target proteins can be quickly purified by means of ion exchange chromatography; exogenous enzymes can be omitted by the fusion expression and purification method as compared with the traditional methods, the ion exchange cost is low, and accordingly the fusion expression and purification method has an excellent application prospect in industrial production on enlarged scales.

The invention relates to the field of environment protection equipment and discloses a gas cleaning tank which comprises a rack, a stirring mechanism and a tank body, wherein a liquid outlet is formedin the bottom of the tank body; the stirring mechanism comprises a stirring shaft, fan blades, an air inlet cylinder and a stepper motor; a piston is arranged inside the air inlet cylinder; an air inlet tube is fixed on the air inlet cylinder; a cavity is formed in the stirring shaft; an air outlet one-way valve and an air inlet one-way valve are fixed on the side wall of the piston or the air inlet cylinder; the stirring shaft penetrates through the tank body, the piston and the air inlet cylinder; the stirring shaft is in threaded connection with the piston; a plurality of beads are arranged between the stirring shaft and the piston; a chute is formed in the side wall of the air inlet cylinder; a sliding block is fixed on the side wall of the piston; a water outlet is formed in the lower end of the stirring shaft; the fan blades are fixed on the stirring shaft; and through holes are formed in the fan blades. By adopting the gas cleaning tank, the sizes of bubbles can be reduced, andthe contact areas of the bubbles with a solution are increased.

The invention discloses an extraction method for grape seed polyphenols, which comprises the following steps: 1) grape seed raw material is dried and then pulverized; 2) the pulverized grape seed raw material is placed in leachate to be subjected to leaching treatment to obtain grape seed raw juice; 3) the grape seed raw juice is filtered to obtain grape seed polyphenols. The extraction method has the benefits as follows: a water-ethanol double aqueous phase system is adopted to replace the traditional leaching method that polyphenols in grape seeds are extracted by a single solvent; as the polarities of leaching solvents can be adjusted better through solvent compounding, not only is the extraction rate of polyphenols in grape seeds increased, but also the selectivity of solvents is improved so as to facilitate follow-up purification, what's more, the solid to liquid ratio of the traditional technology is reduced from 1:20 to 1:1.5 in the whole extraction process, and massive water resource can be saved in the extraction technology.

The invention relates to an artificially synthesized signal sequence and an application thereof. The amino acid sequence of the signal sequence is SEQ ID NO:5. The signal sequence can be added into a mammalian cell expression vector for guiding secreting expression of a mammalian-derived protein, and can increase the expression quantity of an exogenous protein in animal cells.

The invention relates to an artificially synthesized signal peptide and application thereof. An amino acid sequence of the signal peptide is selected from any one of SEQIDNO: 1-5. The signal peptide can be added in a mammal zooblast expression vector for guiding a mammal source protein to secrete and express, and can improve the exogenous protein expression in a zooblast by 2-36 times.

The invention belongs to the field of natural pharmaceutical chemistry and discloses a method for extracting mulberroside A from white mulberry root-bark. The method comprises the following steps of: 1) crushing the white mulberry root-bark, adding an ethanol solution at the concentration of between 30 and 90 percent in an amount which is 6 to 15 times that of the crushed mulberry root-bark, refluxing and extracting for 1 to 3 times, decompressing an extracting solution to recover ethanol, regulating the pH value to 4 to 5, standing and precipitating, and filtering to obtain a concentrated solution; 2) adding the concentrated solution into an ultrafiltration membrane and ultrafiltering, adding permeate into macroporous resin and absorbing, eluting by using an ethanol solution at the concentration of between 40 and 60 percent, collecting eluent, concentrating under reduced pressure, regulating the pH value of a concentrated solution to 4 to 5, filtering, and adding ethyl acetate and extracting to obtain extract liquor; and 3) recovering a reagent from the extract liquor, adding activated carbon and drying, loading into an activated carbon column, performing gradient elution by using ethanol solution, performing thin-layer detection, collecting target ingredients, concentrating, and drying at low temperature to obtain the mulberroside A. The process is easy to operate, is low in production cost and is suitable for industrialized production.

The invention provides a method for preparing a gonadotropin-releasing hormone (GnRH) parallel body and a vaccine in solid-phase conditions. After Lys is coupled on carrier resin, rink amide liners are respectively coupled on the two side chains of the Lys, and two same peptide chains are synthesized simultaneously. Disulfide bond bridging is realized on a solid phase, full-protective oxidation is adopted, and a large amount of impurities caused by the reaction involved by the side chains in the process are avoided.

The invention discloses a sewage treatment device for a municipal pipeline network, which comprises a sedimentation tank, a sludge mixing tank, a purification tank and an air-dissolving tank. The sedimentation tank is connected to the sludge mixing tank through a mud delivery pipe, and a second Stirring chamber and pressurized pipe, the second stirring chamber is provided with a stirring mechanism, the pressurized pipe is provided with a conveying screw, the end of the conveying screw is provided with a third motor, and a filter ball is provided in the sedimentation tank, which is connected by a sewage pump Purification tank, the purification tank is divided into a first mixing chamber and a purification chamber through a partition, a permeable membrane is set on the partition, the first stirring chamber is connected to the sludge mixing tank through a mud guide pipe, and the upper and lower intervals in the purification chamber are provided with drug discharge pipes and The deflector and the purification pool are connected to the dissolved air tank through the dissolved air water pump. The invention has simple structure and strong practicability, can not only improve the sewage purification treatment effect, make sludge and sewage be separated rapidly, but also facilitate the recycling of sludge and sewage, reduce waste of resources and realize recycling.

The invention relates to the technical field of biology and discloses a whole genome cloning method of pelteobagrus fulvidraco bacillus-shaped virus. The method comprises the following steps of: designing 14 specific primers for reverse transcription and 14 pairs of specific primers for PCR, wherein the primer sequences are shown as SEQ ID No.1-SEQ ID No.14; extracting pelteobagrus fulvidraco bacillus-shaped virus RNA; taking the extracted viral RNA as a template, respectively adding the extracted viral RNA into a reverse transcription reaction system and a PCR reaction system by using the primers, cloning the whole genome of the pelteobagrus fulvidraco bacillus-shaped virus by sections under reaction conditions, wherein 13 fragments of the amplified product are 2000bp in size, one fragment is 1000bp in size, a total of 14 fragments contain the entire genome sequence of the pelteobagrus fulvidraco bacillus-shaped virus. The invention provides a cloning method of the whole genome of thepelteobagrus fulvidraco bacillus-shaped virus. The method not only can be used for rapid cloning of the whole genome of the pelteobagrus fulvidraco bacillus-shaped virus, but also can be used for researching molecular biology pathogenic mechanism and molecular epidemiology investigation of the pelteobagrus fulvidraco bacillus-shaped virus, and is convenient to use and has good effect.

The invention belongs to the field of organic synthesis, and particularly relates to a method for synthesizing cyclic carbonate and derivatives thereof, which comprises the following step of: reactingan epoxy compound shown in a formula I with carbon dioxide under the catalysis of a catalyst shown in a formula II to obtain a target product. The product prepared by the method has the advantages ofhigh yield, high purity, low catalyst cost and mild synthesis conditions, and the obtained product has no metal residue.

The invention provides a method for concentrating and separating protein in purple perilla cake meal through a two-stage froth flotation method. The method comprises the following steps that 1, purpleperilla cake meal leaching liquor is prepared; 2, in the first-stage froth flotation, a froth flotation tower provided with a hourglass-shaped component is used for strengthening froth liquid drainage, the enrichment ratio of purple perilla protein is increased, and an obtained concentrated solution can be directly used as a raw material for producing purple perilla protein powder; and 3, the first-stage residual liquid is used as the feed liquid in the second-stage froth flotation, so that the recovery effect of the purple perilla protein is further improved. The method has the advantages ofgreen process, low energy consumption, low investment, simple steps and the like, effectively solves the problem of concentration and separation of the purple perilla protein, and has good economic and social benefits.

The invention relates to a preparation method of dehydro-harmine, which comprises the steps of smashing harmel seeds which are taken as a material, adding appropriate ammonia water, mixing uniformly, carrying out ammoniation for 10-20 hours, adding a 70-80% carbinol solution with 3-5 times as quantity as that of the material for soaking for 2-3 times, filtering soaking liquid, adding a chloroform solution, collecting a chloroform layer, recycling the chloroform, carrying out separation preparation on solid by a petroleum ether-ethyl acetate-carbinol-hydrosolvent system through using a high-speed counter current chromatograph, collecting dehydro-harmine fluid, recycling reagents, and drying under a low-temperature vacuum condition, so that pure dehydro-harmine is obtained. The method is applicable to the preparation of the dehydro-harmine with high purify.

The invention discloses a production system for preparing formaldehyde, paraformaldehyde, methylal and hexamethylene tetramine from methanol, and belongs to the technical field of compound preparation. A process includes the steps that S1 methanol is used as a raw material and is subjected to recovery to obtain a low-concentration formaldehyde solution through oxidation, and unrecovered formaldehyde mixed gas is absorbed by an absorption tower to obtain a light aldehyde solution; S2 methanol and the low-concentration formaldehyde solution are used as raw materials and sequentially processed bya resin reactor, a reaction tower, a rectification tower and a cooler to obtain methylal; S3 the low-concentration formaldehyde solution in the process S1 is concentrated by secondary evaporation toobtain a high-concentration formaldehyde solution, and the high-concentration formaldehyde solution is vacuum-dried to obtain paraformaldehyde; and S4 liquid ammonia is used as a raw material, after vaporization, the liquid ammonia reacts with the light aldehyde solution in the step S1, and evaporative crystallization, centrifugation and drying are performed to obtain hexamethylene tetramine. Theproduction system can improve the effective utilization of resources such as the raw materials, reduce the discharge of pollutants, reduce environmental pollution, effectively reduce the production cost of enterprises and improve economic benefits.

Methods for the synthesis of 2-thiohistidine or a derivative thereof of the formula (I), or of a physiologically acceptable salt, a tautomer, a stereoisomer or a mixture of stereoisomers in any proportions thereof, from a compound of the formula (II) or a physiologically acceptable salt, a tautomer, a stereoisomer or a mixture of stereoisomers in any proportions thereof, by cleavage reaction in the presence of a thiol at a temperature higher than or equal to 60° C. The invention also relates to compounds of the formula (II) and a method for the synthesis thereof.

The invention provides a foam separation method for concentrating and separating rosmarinic acid in labiatae (preferably perilla leaves). The method comprises the following steps: 1, a perilla leaf extract is prepared; 2, in the first-stage foam separation, a betaine type surfactant is used as an auxiliary agent, rosmarinic acid is effectively collected to a gas-liquid interface, concentration and separation of perilla rosmarinic acid are facilitated, and an obtained concentrated solution can be directly used as a raw material for producing perilla rosmarinic acid powder; and step 3, second-stage foam separation is carried out by taking the first-stage residual liquid as a feed liquid, so that the recovery effect of the rosmarinic acid in the perilla frutescens is further improved. According to the two-stage foam separation, the concentration (enrichment) ratio of the rosmarinic acid can be greatly increased (7.5-14.0), and the recovery rate can be increased (86%-99%). The method has the advantages of greenness in process, no addition of organic reagents, low energy consumption, simplicity in operation and the like, effectively solves the problem of concentration and separation of rosmarinic acid in perilla frutescens, and has good economic and social benefits.

The invention belongs to the field of health care products and in particular relates to a ginseng oral liquid for tonifying qi and calming the nerve and a preparation method thereof. The oral liquid is prepared from ginseng total glycoside extract, astragalus root extract, powdered eleuthero extract, poria cocos extract, platycladi seed extract, barbary wolfberry fruit extract, spina date seed extract and small molecule ginseng polypeptide-zinc chelate. The ginseng oral liquid has effects of maintaining mental tranquility and nourishing the qi, soothing the nerves for relieving depletion, tonifying the spleen and benefitting the lung, enriching the blood and nourishing the yin, quieting the heart and invigorating the brain; and the ginseng oral liquid is suitable for symptoms such as spirit drooping caused by the lack of fluid and blood, qi and yin deficiency, haggard expression, insomnia and forgetfulness and nervous breakdown. The traditional Chinese medicine extraction process has high efficiency and low cost, saves energy, is green and environmentally friendly, and further improves the economic value of Chinese medicinal materials. The prepared oral liquid is efficient, non-toxic and convenient to take; particularly due to the adding of the small molecule ginseng polypeptide-zinc chelate easily absorbed by the human body, users can simultaneously supplement amino acids andtrace elements required by the human body, and the nutritional value of the oral liquid health care product is enriched.

The invention discloses an environmental-protection garbage incineration device. The device comprises a base; a garbage incineration furnace is fixedly arranged at the top of the base through a bracket; a first drawer is arranged at the inner bottom of the garbage incineration furnace; the bottom of the first drawer is opened, and the opened place is fixedly connected with a ventilation net; the left wall of the garbage incineration furnace is fixedly connected with a feed pipe; the feed pipe is positioned above the first drawer, and is inclined to the right lower side; the left end of the feed pipe is fixedly connected with a feed hopper; a shelf is fixedly connected to the top in the garbage incineration furnace; a heating water tank is arranged above the shelf; the right lower angle ofthe heating water tank is connected with a water inlet pipe; the left upper angle of the heating water tank is connected with a hot water pipe; and the hot water pipe and the water inlet pipe both extend outside the garbage incineration furnace. The device heats water through garbage incineration for resource reutilization to achieve energy conservation and environmental protection; and smoke generated in garbage incineration preheats cold water, so that the heating speed of hot water is higher, and the energy utilization rate is further increased.

The invention discloses a fermentation medium and a fermentation method for preparing recombinant human interferon alpha2b, including a fermentation medium formula which is suitable for constitutive expression recombinant human interferon alpha2b engineering bacteria and a fermentation method thereof; the fermentation medium formula comprises the following components in parts by weight: 25-35 parts of tryptone, 15-25 pats of yeast extract, 4-8 parts of disodium hydrogen phosphate, 2-4 parts of mono-potassium phosphate, 0.4-0.6 parts of ammonium chloride, 0.4-0.6 parts of sodium chloride, 0.2-0.4 parts of magnesium sulfate, 0.05-0.15 parts of calcium chloride and 10-20 parts of glycerol; the glycerol is used as the only carbon source during a culture process of the fermentation method, and the expression rate of target protein is regulated by using proper phosphate concentration, therefore the excellent growing status of the engineering bacteria is ensured, and the high-density culture and high-efficiency expression are achieved.

The invention discloses a sewage purification device. The device comprises a pretreatment component, a sterilization component, a purification component, a water storage tank, a water outlet, a liquidometer and an electric control cabinet. According to the device, through the arrangement of the pretreatment component, sewage enters the device from a raw water inlet pipe, a pretreatment filter screen pretreats impurities in the sewage, the impurities fall into a waste box, thus, the purpose of pretreatment is achieved, subsequent purification of the sewage is benefited, the purification effectof the purification device is improved, and the service life of the purification device is prolonged; a second valve is opened regularly to discharge the impurities in the waste box from a blow-off pipe, and thus normal running of the pretreatment component is guaranteed; and through the arrangement of the sterilization component and the purification component, when a button switch is pressed, a UV lamp performs sterilization on the sewage, then the sewage enters a purification box and is purified after passing through a primary filter screen, an activated carbon filling layer and a nanofiltration membrane layer, thus, the purpose of thoroughly purifying the sewage is achieved, purified water reaches a recycling standard, and waste of water resources is avoided.

The invention provides an expression purification method restriction enzyme SmaI. The method comprises the following steps that recombinant bacteria containing restriction enzyme SmaI expression fragments are constructed, induced culture is conducted, bacterium bodies are fragmented, a product is purified and recycled, and the restriction enzyme SmaI is obtained; and the purifying modes comprise combination of at least two of Ni ion affinity chromatography, anion exchange chromatography or heparin-affinity chromatography. Through combination of multiple purifying modes, the restriction enzymeSmaI with the purity larger than 98% of obtained.

The invention discloses a method for extracting rebaudioside A, stevioside and stevioside polyphenol from stevia rebaudiana and application thereof. The method comprises the following steps: adding saturated lime water into a leaching solution obtained by leaching stevia rebaudiana powder with hot water to adjust the pH value, standing and precipitating, and performing centrifugal separation; performing post-treatment on the centrifuged supernate to obtain a stevioside crude product, then adding n-propyl alcohol, heating, filtering while hot, and respectively purifying a filtrate and a filter cake to obtain stevioside and rebaudioside A; and adding diluted hydrochloric acid into the centrifuged precipitate to dissolve, adjusting the pH value, centrifuging, taking the supernatant, and purifying with polyamide resin to obtain the stevia polyphenol. According to the method, two main components in stevioside can be efficiently separated by utilizing the characteristic that rebaudioside A and stevioside have different solubilities in n-propanol; and the purification time can be shortened by crystallizing the rebaudioside A crude product with the purity of 80% or above, the situation that rebaudioside A is partially dissolved in mother liquor and cannot be fully separated out during direct crystallization is avoided, and stevioside with the content of 95% or above can be obtained.

The invention relates to the technical field of biology, in particular to a method for achieving soluble expression of recombinant CXCL9 (C-X-C motif chemokine 9) protein in Escherichia coli by fusionof a solubilizing label and Intern. The target protein is expressed by fusion with the solubilizing label and the Intein with the self-shear function, and soluble protein and shear release of the target protein are realized. Compared with a known method of expression of recombinant CXCL9 protein in Escherichia coli, the method has the advantages that the process of inclusion volume degeneration renaturation can be avoided, introduction of protease is not needed, a target product with the completely naturally sequence can be obtained, and good application prospect for the enlarged-scale industrial production is achieved.

The invention relates to an injection-grade echinacea extractive and an injection thereof. The extractive is prepared according to the following method: 1) crushing echinacea for usage; 2) warmly dipping the crushed echinacea powder with an aqueous solution containing a base to extract for two times, filtering, discarding medicine residues, and merging the filtrate for usage; 3) applying the filtrate obtained in the step 2) to anion resin, washing with water, then eluting with an ethanol solution containing an acid, collecting the eluate, and recovering ethanol in a reduced-pressure manner, and obtaining a concentrate for usage; and 4) performing salting out on the concentrate obtained in the step 3), refrigerating, filtering, discarding filtrate, and washing the precipitate with water, so as to obtain the extractive. The provided injection is high in content of intermediates chicoric acid and caftaric acid, low in cost and good in medicine effect.

The invention relates to a method for preparing xylitol by fermenting xylose secondary mother liquor, the method comprises the following steps: fermenting mixed liquor of the xylose secondary mother liquor and xylose concentrated liquor, and replacing subsequent glucose fed-batch with the xylose secondary mother liquor in the fermentation process, so that the stable glucose content in a fermentation system is met, and part of xylose can be provided, the glucose consumption can be saved, the cost is reduced, the relative stability of the system xylose can be maintained, and the overall conversion rate is improved. With applicaiton of the xylose secondary mother liquor added in the modes of mixed fermentation, fed-batch and the like, the content of miscellaneous sugars such as arabinose and mannose in a fermentation system is always within the metabolizable range of fermentation strains, subsequent purification is facilitated, and the purity of xylitol can be improved.

The invention belongs to the field of health care product, and especially relates to a gen-seng oral liquid capable of nourishing yin and tonifying yang, and a preparation method thereof. The gen-sengoral liquid capable of nourishing yin and tonifying yang is prepared from gen-seng total glycoside extract product, prepared rehmannia root extract product, polygonatum rhizome extract product, Polygonum multiflorum extract product, ligustrum lucidum extract product, Chinese wolfberry extract product, dioscorea rhizome extract product, and small molecule gen-seng polypeptides, is capable of nourishing yin and inhibiting deficiency fire, nourishing liver and kidney, boosting essence and replenishing the marrow, nourishing yin and clearing lung-heat, quenching thirst and relieving cough, and calming the nerves, and is especially for people with yin deficiency to drink. The preparation method is high in extraction efficiency, and low in cost, is capable of saving energy, is green, is friendly to the environment, is capable of increasing the economical value of traditional Chinese medicinal materials; the gen-seng oral liquid is high in efficiency, is nontoxic, is convenient to drink; small molecule gen-seng polypeptide-zinc chelates are added, so that amino acids and trace elements needed by human body can be supplied, and the nutritional value of the gen-seng oral liquid is increased.