The invention relates to a multicistron
plasmid type reverse transcription
virus vector and a construction method thereof, and belongs to the field of
gene engineering. The multicistron
plasmid type reverse transcription
virus vector is obtained through a
gene recombination method, and the method comprises the steps that a
recombinase gene, a simian
virus large T
antigen gene, a
human telomerase gene which are fused with an
estrogen receptor and five cistrons of a strengthened
green fluorescent protein gene fused with
thymidine kinase are inserted into the reverse transcription virus vector, and the a recombinant
plasmid is obtained. Compared with the prior art, by means of an immortalized
cell line established through the plasmid type reverse transcription virus vector, non-transformed normal cells can be obtained after
tamoxifen drug treatment, and meanwhile by means of a
thymidine kinase suicide gene mechanism, non-returned cells can be made to suicide after
ganciclovir drug treatment; therefore, complete non-transformed normal cells are obtained. The multicistron plasmid type reverse transcription virus vector and the construction method thereof have the advantages that the
biological safety of the recombinant reverse transcription virus vector is guaranteed, high expression of multicistron selectivity is promoted, and follow-up screening of a positive
cell group and detection of multicistron expression are facilitated.