The invention belongs to the technical field of catalysts and relates to a tungstenic functional mesoporous molecular sieve catalyst for preparation of glutaric acid based on glutaraldehyde oxidationand a preparation method of the catalyst. The catalyst is a functional mesoporous molecular sieve SBA-15 supported tungstic catalyst (W-SBA-15), and tungsten is introduced into a framework of a functional mesoporous molecular sieve SBA-15 with a post-synthesis method. The method comprises steps as follows: the mesoporous molecular sieve SBA-15 is dispersed in a solvent and stirred, an organosilaneagent is added, a mixture is stirred, subjected to reflux, filtered, dried, subjected to Soxhlet extraction and dried, dichloromethane is added, stirring is performed, trifluoromethanesulfonic acid is added, stirring, filtering, ethanol rinsing and drying are performed, then a tungsten source is added, stirring, filtering, water washing and drying are performed, and the catalyst is obtained. Themethod is easy to operate. The prepared tungstenic functional mesoporous molecular sieve SBA-15 heterogeneous catalyst is highly ordered in porous structure, tungsten species is highly dispersed, andultrahigh stability is obtained. The prepared W-SBA-15 heterogeneous catalyst is applied to a reaction of preparation of glutaric acid based on glutaraldehyde oxidation, conversion rate of glutaraldehyde can reach 99% or higher, yield of glutaric acid can reach 98% or higher, and the catalyst can be repeatedly used 20 times or more with unchanged activity.