Flavone C-glycoside extract and preparation method thereof

A technology of flavonoid carbon glycosides and extracts, applied in the field of medicine and health food, can solve the problems of complex composition, low product purity and total transfer rate, affecting the practical application of ingredients, etc.

Inactive Publication Date: 2013-06-19
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the complex composition of flavonoid glycosides and other coexisting components in plants, the preparation proce

Method used

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  • Flavone C-glycoside extract and preparation method thereof
  • Flavone C-glycoside extract and preparation method thereof

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Embodiment 1

[0017] Embodiment 1: Investigation on acid hydrolysis reaction of flavonoid carbon glycoside monomer

[0018] The reference substances isoorientin and isovitexin are commercially available. Reference substance 2″-Galactosylorientin, orientin, and vitexin are self-made, and their purification process can be found in: Li Shengyin, Research on Chemical Constituents of Nasturtium, Soochow University Master’s Thesis, 2008.

[0019] Acid hydrolysis reaction: Weigh 5 mg of each reference substance, add 10 mL of 2M HCl respectively, react in water baths at two temperatures of 60°C and 80°C for 6 hours, and neutralize with 4M NaOH to pH 5-7. HPLC analysis of components such as orientin and vitexin in the hydrolyzate, chromatographic analysis conditions: ODS chromatographic column (4.6 * 250mm); Acetonitrile-0.1% acetic acid-water is mobile phase gradient elution (0-5min, 13% acetonitrile ; 5-25min, 13-15% acetonitrile; 25-30min, 15-18% acetonitrile; 30-50min, 18-28% acetonitrile; 50-6...

Embodiment 2

[0022] Example 2: Investigation on the Acid Hydrolysis Process of Trollius Component TC-A

[0023] The component TC-A of nasturtium lotus mainly contains 2″-galactosylorientin (content 30.85%), orientin (content 50.49%) and a small amount of vitexin (content 12.67%). For the purification process, please refer to: Liu Jiangyun Etc. A flavonoid carbon glycoside composition and its preparation method and application. Patent application number CN201110055655.5. The main process flow is: take 100g of dried nasturtium medicinal material, heat and reflux (1.5h) with 1000mL of water to extract twice , the extracts were combined and concentrated under reduced pressure to 1.5 g crude drug / mL, added an equal volume of 95% ethanol, stirred continuously, and filtered to obtain a filtrate; the filtrate was concentrated under reduced pressure, added water to make a sample solution of 1.0 g crude drug / mL, and AB-8 macroporous resin column, respectively eluted with 1500mL water, 2000mL30% etha...

Embodiment 3

[0032] Example 3: Investigation on the Acid Hydrolysis Process of Trollius Component TC-B

[0033] The nasturtium component TC-B mainly contains orientin esters and vitexin ester derivatives, including 2″-O-(2″′-methylbutyl) isoflavin, 2″-O- (3″′, 4″-dimethoxybenzoyl) isoflavin, 2″-O-(2″′-methylbutyl) vitexin, 2″-O-(2″′ -methylbutyl)orbitoside, 6″′-hydroxymethylglutaryl-2″-O-β-L-galactosylorbitoside, 6″′-hydroxymethylglutaryl-2″- O-β-L-galactose vitexin and so on.

[0034] Investigation of hydrolysis conditions: Weigh 1 g of TC-A sample, add 100 mL of 2.0M HCl-50% ethanol respectively, react in a water bath at 80°C for 6 hours, and neutralize to pH 5-7 with 4M NaOH. The reaction solution was further coated with AB-8 resin, and eluted with water, 30% ethanol, and 70% ethanol in sequence, and the eluted sites of 30% ethanol were collected, concentrated, and dried to obtain TC-BH product (0.56 g).

[0035] Results: Other flavone carbon glycoside derivatives in the reaction pro...

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Abstract

The invention discloses a preparation method of a high-purity flavone C-glycoside extract. The preparation method mainly comprises the following steps of: selecting vegetable drugs rich in flavone C-glycoside, extracting by adopting water or an ethanol-water mixed solvent, and optionally further separating, purifying and concentrating as required to obtain a flavone C-glycoside extract concentrated solution, wherein the concentrated solution is dissolved and diluted into a defined amount of acid water or an alcohol-containing aqueous solution to carry out an acid hydrolysis reaction, the obtained extract hydrolysate is further separated and purified by adopting fillers such as macroporous resin and the like, and high-purity (more than 60%) flavone C-glycoside extract is obtained. The preparation method disclosed by the invention adopts an acid hydrolysis control technology, and other flavone C-glycoside derivatives in plants can be converted into corresponding flavone C-glycoside components, so that content and purity of a flavone C-glycoside component are obviously increased, a process is simple, operability is strong, and industrialization amplification is easy to achieve.

Description

technical field [0001] The invention relates to a flavonoid carbon glycoside extract, and also relates to a preparation method of the flavonoid carbon glycoside extract, which belongs to the field of medicine and health food. technical background [0002] Flavone carboglycosides are a class of flavonoid glycosides composed of the 6 or 8 positions of flavone aglycones and the 1 position of sugar groups connected by carbon bonds. Representative compounds such as vitexin, isovitexin, orientin, Isorientin, etc. This type of ingredients has good pharmacological activities such as anti-oxidation and anti-inflammation, and is one of the main active ingredients of various medicinal plants, such as hawthorn leaves, bamboo leaf flavonoids, nasturtium, weed, blueberry, etc. There are many reports on the preparation of such natural products by separation techniques such as macroporous resin purification, column chromatography or high-speed countercurrent chromatography. However, due t...

Claims

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Application Information

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IPC IPC(8): C07D407/04
Inventor 刘江云孙燕苑红燕郝丽莉蔡金娜蔡培烈杨世林
Owner SUZHOU UNIV
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