79 results about "Structural biology" patented technology

The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore in the absence of requiring a terminating nucleotide. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of drug discovery, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The invention relates to the field of biochemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-β structures and the biological role of these cross-β structures. In one embodiment, the invention discloses a method for modulating extracellular protein degradation and / or protein clearance comprising modulating cross-β(beta) structure formation (and / or cross-β structure-mediated activity) of the protein present in the circulation.

The invention relates to the field of biochemistry, molecular biology, structural biology and medicine. More in particular, the invention relates to cross-β structures and the biological role of these cross-β structures. In one embodiment, the invention discloses a method for modulating extracellular protein degradation and / or protein clearance comprising modulating cross-β(beta) structure formation (and / or cross-β structure-mediated activity) of the protein present in the circulation.

An improved method for producing humanized antibody or an antigen binding fragment thereof is described. The method, designated framework-assembly, bypasses the reliance on structural biology and the construction of large libraries. It is easier to implement and more efficient than the rational design and empirical methods. Also described are humanized antibodies produced by the method and related framework-assembly library.

A novel three-dimensional reconstruction system comprises a workflow presenting module of an Appion system, a data instruction conversion module, a data configuration module, a grid job queue module of an LSF system, an LSF scheduling library module, an LSF billing library module and a network resource pool module. The novel system based on the LSF system, the Appion system and a cryo-electron microscopy technology achieves three-dimensional reconstruction. The system ingeniously and organically combines the Appion system, a cryo-electron microscopy biological structure research technology and a grid calculation technology based on an LSF, and forms a novel cryo-electron microscopy three-dimensional reconstruction technology. The technology is mainly characterized by achieving a three-dimensional reconstruction assembly line technology, and the assembly line technology can effectively reduce work intensity of electron microscopic image analysis of biologic samples and effectively improve research efficiency of an electron microscopy three-dimensional reconstruction method in structural biology.

The invention discloses a cDNA overall length nucleotide sequence of a human EBLN-1 gene and a cloning method thereof. According to the invention, the cloning method comprises the following steps of: respectively amplifying a 5' end fragment and a 3' end fragment of human EBLN-1 gene according to 5'RACE and 3'RACE technologies and sequencing; then, splicing to obtain the cDNA overall length nucleotide sequence of the human EBLN-1 gene according to the 3' end sequence, the 5' end sequence and a known cDNA part sequence; performing analysis of an opened reading frame and an untranslated region; and deducing to obtain an amino acid sequence which codes the EBLN-1 protein according to the opened reading frame. The cDNA overall length nucleotide sequence of the human EBLN-1 gene can be used for subsequently expressing a recombinant protein for preparing corresponding antibodies or performing structural biological study, for studying EBLN-1 gene function through RNA interference and overexpression or further studying the effect of the untranslated region in regulation of gene expression, and finally laying a foundation for illustrating the physiological action of EBLN-1 gene in human body and the relation with diseases, in particular mental diseases.

The invention herein disclosed provides for methods for the synthesis of polymers from monomers. In particular the method provides for the synthesis of polynucleotides from mononucleotides in the absence of catalytic enzymes. The method comprises providing an aqueous solution having a plurality of phospholipid molecules and monomer molecules; subjecting the aqueous solution to fluctuating temperature conditions; subjecting the aqueous solution to fluctuating cycles of drying and hydrating conditions; subjecting the aqueous solution to fluctuating [H+] conditions; the fluctuating conditions thereby allowing formation of a chemical bond between at least two monomers to create a polymer. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The invention herein disclosed provides for methods for the synthesis of polymers from monomers. In particular the method provides for the synthesis of polynucleotides from mononucleotides in the absence of catalytic enzymes. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The present invention provides methods for controllably forming a layer of amorphous ice and other amorphous solids on a substrate, and also provides cryoelectron microscopy (cryo-EM) sample preparation methods and systems that utilize in vacuo formation of amorphous ice and other solids. Formation of the amorphous solid layer can be independent of the deposition of sample molecules to be analyzed using electron microscopy, and allows for the generation of a uniformly thick layer. Optionally, mass spectrometry instruments are used to generate and purify molecules deposited on the generated amorphous solid layer. The techniques and systems described herein can deliver near ideal cryo-EM sample preparation to greatly increase resolution, sensitivity, scope, and throughput of cryo-EM protein imaging, and therefore greatly impact the field of structural biology.

The invention discloses an application of a multifunctional graphene grid in three-dimensional reconstruction of a cryoelectron microscope. The preparation method of the multifunctional graphene grid comprises the following steps: etching a copper foil covered with a graphene film grown by CVD (Chemical Vapor Deposition) to obtain the graphene grid; performing oxygen plasma treatment on the graphene grid; then, adopting an MES buffer solution containing EDC and NHS treat the graphene grid; activating the graphene grid by adopting nitrilotriacetic acid and biotin; and finally reacting with nickel salt. The multifunctional graphene grid can specifically anchor a target biomolecule on the surface of the multifunctional graphene grid, so that the interface problem of air and water is avoided, the orientation distribution of particles can be controlled, and by constructing related protein purification tags to different directions of each biomacromolecule, abundant Euler angles are provided, and the structural analysis of biomacromolecules is more reliable and more efficient, so that the multifunctional graphene grid can promote the universality and repeatability of application of a cryoelectron microscope in structural biological analysis.