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Epitope polypeptide combination capable of inducing immunity and application thereof

A technology of epitope polypeptide and antigen epitope, which is applied in the direction of application, medical preparations containing active ingredients, peptides, etc., can solve the problems of aggravating immunopathological damage, imbalance of cellular immunity, non-neutralization or inhibition of virus replication, etc.

Pending Publication Date: 2021-09-10
SHANTOU UNIV MEDICAL COLLEGE
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Problems solved by technology

However, the immune response induced by most epitopes does not have the effect of neutralizing or inhibiting virus replication, which may induce negative risks such as cellular immune imbalance, aggravate inflammatory response, induce immunopathological damage, antibody-dependent infection and enhance ADE, and at the same time dilute Immunoprotective Effects of Immunoprotective Epitopes

Method used

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  • Epitope polypeptide combination capable of inducing immunity and application thereof
  • Epitope polypeptide combination capable of inducing immunity and application thereof
  • Epitope polypeptide combination capable of inducing immunity and application thereof

Examples

Experimental program
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Effect test

Embodiment 2

[0062] Example 2 result

[0063] 2.1 Determination of new coronavirus candidate vaccine strains

[0064] The surface glycoprotein [Severe acute respiratory syndrome coronavirus 2] (NC_045512.2) Spike protein published in the NCBI database was selected as the template for cell epitope prediction. The currently uploaded 579 amino acid sequences of different Spike proteins were collected through the NCBI database as templates for conservation analysis, and all protein data were collected in FASTA file format.

[0065] 2.2 Preliminary screening of novel coronavirus epitopes

[0066] Table 1 lists five identified B cell epitopes (SEQ ID No. 1-5). Table 2 lists 6 Th epitopes (SEQ ID No.6-11), and Table 3 lists 5 CTL epitopes (SEQ ID No.12-17). The conformational positions of all candidate epitopes in the S protein are as follows figure 1 As shown in A, it is of great significance to obtain neutralizing antibodies that can neutralize the binding domain of Spike protein and ACE2. ...

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Abstract

The invention discloses an epitope polypeptide combination capable of inducing immunity and application thereof, belongs to the technical field of biology, and aims to carry out molecular design of related vaccines by utilizing immunoinformatics on the basis of epitope analysis optimization. Based on a structural antigen epitope vaccine design strategy, a B cell epitope, a Th epitope and a CTL epitope on a new coronavirus S protein are determined through immunoinformatics to induce a main neutralizing antibody, activate cellular immune response and induce body fluid and cellular immune balance. The epitope vaccine is designed through connection of a molecular adjuvant and candidate antigen epitopes, antigenicity, physicochemical properties, protein secondary structure and tertiary structure modeling of the epitope vaccine are analyzed, vaccine conformation B cell epitopes are analyzed by means of a structural biological tool, and immune response characteristics of the vaccine are verified through molecular docking with TLR4 and immune response simulation stimulation. Information analysis results show that the designed candidate epitope combination has well balanced humoral immune and cellular immune response capabilities.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an immune-inducing epitope polypeptide combination and its application. Background technique [0002] Novel coronavirus (SARS-CoV-2) infection can cause new coronary pneumonia (Corona Virus Disease 2019, COVID-19), which belongs to Betacoronavirus (Betacoronavirus) in classification, and is a single-stranded positive-sense RNA virus. The main manifestations of the disease are fever, dry cough, and fatigue, often accompanied by shortness of breath and dyspnea. In severe cases, COVID-19 can lead to severe acute respiratory distress syndrome, septic shock, functional failure and even death. The outside of the spherical new coronavirus with crown spikes is a shell composed of various proteins. The structure of the coronavirus mainly includes spike protein (Spike, S), membrane protein (Envelope, E), nucleoprotein (Nucleoca psid, N) and envelope protein (Membrance, M). The S protein form...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/165C12N15/50A61K39/215A61P31/14
CPCC07K14/005A61K39/12A61P31/14C12N2770/20022C12N2770/20034
Inventor 李蕊邓辉雄李雁嫘宋鑫利谷李铭何绚婷王婷婷代剑平王革非
Owner SHANTOU UNIV MEDICAL COLLEGE
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