35 results about "Nucleotide base sequence" patented technology

The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

The invention discloses a PCR (Polymerase Chain Reaction) molecular marking method for identifying allele mutation of rice long-grain gene qGL3, and belongs to the technical field of agro-biological engineering. According to the PCR molecular marking method, four specific molecular marking primers are designed and synthesized for PCR amplification of DNA of different rice based on the difference of long-grain and large-grain variety TD70 and short-grain and small-grain variety Kasalath on the nucleotide base sequence of the long-grain gene qGL3, the amplified product is subjected to electrophoresis detection with 2.0% of agarose gel, and DNA brands of different dimension represent different allele types of qGL3 after DuRed nuclear acid developing. With the adoption of the molecular marking method, the difference of different alleles of the long-grain gene qGL3 in rice germplasm resources or breeding population can be quickly and accurately identified; in addition, the selection efficiency for the long-grain homozygous genotype qGL3-TqGL3-T is obviously improved; and therefore, the coordinative improvement of the appearance quality and output traits of rice can be realized.

The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

The present invention concerns oligonucleotides containing one or more modified nucleotides which increase the binding affinity of the oligonucleotides to target nucleic acids having a complementary nucleotide base sequence. These modified oligonucleotides hybridize to the target sequence at a faster rate than unmodified oligonucleotides having an identical nucleotide base sequence. Such modified oligonucleotides include oligonucleotides containing at least one 2′-O-methylribofuranosyl moiety joined to a nitrogenous base. Oligonucleotides can be modified in accordance with the present invention to preferentially bind RNA targets. The present invention also concerns methods of using these modified oligonucleotides and kits containing the same.

Method for analyzing a phyletic lineage of scallop, which comprises the steps of sequencing a mitochondrial DNA of the scallop to determine a nucleotide base sequence of the mitochondrial DNA, wherein the nucleotide base sequence includes a non-coding region containing a nucleotide base substitution locus which shows a sequence polymorphism indicative of a particular lineage of the scallop. Mitochondrial DNA of the scallop is amplified by PCR, using a suitable primer set designed on the basis of nucleotide base sequences conserved among known shellfish mitochondrial 16S rRNA and 12S rRNA genes, followed by sequencing to determine a non-coding region therein. In such non-coding region, a nucleotide base substitution locus is located, whereby a particular lineage of the scallop is determined. Based on those steps, a Japanese scallop, Patinopecten yessoensis, is analyzed as to the nucleotide base sequence and lineage thereof.

The invention belongs to the fields of a gene sequence and an obtaining method of the gene sequence, and particularly relates to an obtaining method of an SNP (single nucleotide polymorphism) site and a CAPS (cleaved amplified polymorphic sequence) mark interlocked with a citrullus lanatus fruit bitter taste gene Bt (bitterness). The SNP site and the CAPS mark interlocked with the citrullus lanatus fruit bitter taste have nucleotide base sequences in SEQ ID NO:1-2 in a sequence table. The citrullus lanatus fruit bitter taste gene is primarily positioned by screening a bitter taste single plant in an RILs colony and combining with the high-density genetic map information of the citrullus lanatus; the candidate SNP site interlocked with the character is combined with separate colony verification analysis and natural colony verification analysis; the mark tightly interlocked with a target character is obtained; the SNP site and the CAPS mark can be applied to improvement of citrullus lanatus variety and molecular assisted breeding; a technical support is provided for molecular breeding of the citrullus lanatus quality; and meanwhile, the traditional gene positioning time is greatly shortened.

The invention relates to the fields of gene sequences and acquiring methods thereof, and concretely relates to SNP loci linked with a blight resistant gene Fon-1 in a watermelon, markers thereof, and acquiring methods of the loci and the markers. The SNP loci linked with a blight resistant gene Fon-1 in a watermelon and the markers thereof have nucleotide base sequences represented by SEQ ID NO:1-8 in a sequence table. The acquiring method of the loci comprises the following steps: 1, selecting a tested material; 2, carrying out preliminary positioning of the blight resistant gene Fon-1 in the watermelon; 3, acquiring candidate SNP; and 4, acquiring the SNP loci closely linked with the blight resistant gene Fon-1 in the watermelon, and verifying the candidate SNP loci. According to the invention, the phenotype identification and the molecular detection of F2 generation separation populations and natural populations are carried out through utilizing a series of markers to confirm the close linkage degree of the series of the SNP loci with target properties; and the SNP loci are utilized to design the markers respectively, and the markers can be utilized to carry out the initial-stage screening of the kind in order to reach a molecule assisted breeding purpose, so the breeding period is substantially shortened, and an important scientific research meaning is possessed.

The invention discloses a CAPS (cleaved amplified polymorphic sequence) molecular marking method for identifying solanum lycopersicum with purple black striped pericarp and an application. The sequence of a molecular marker carried by the solanum lycopersicum with purple black striped pericarp is a nucleotide base sequence shown as SEQ ID NO.1, PCR amplification is performed with genomic DNA of the solanum lycopersicum with purple black striped pericarp as a template, nucleotide base sequences shown as SEQ ID NO.3-4 are amplification primers, amplification and cleavage are performed, a specific band different from other solanum lycopersicum pericarp colors can be obtained after cleavage, and the molecular marking method is applied to breed improvement and breeding of the solanum lycopersicum. Compared with the prior art, the CAPS molecular marking method has the advantages that the polymorphism of a cleaved fragment is observed through PCR amplification, cleavage and gel electrophoresis separation, the step of membrane transfer in the traditional RFLP (restricted fragment length polymorphism) analysis is omitted, the operation is simplified, the precision of the RFLP technology is kept, the polymorphism detecting probability is higher, the solanum lycopersicum with purple black striped pericarp is obtained rapidly and applied to breed improvement and assisted breeding, and the method has important theoretical and practical meanings for culturing new varieties of solanum lycopersicum containing rich anthocyanidin.

The invention belongs to the field of gene sequences and obtaining methods thereof, and particularly relates to a gene sequence a for causing watermelon bisexual flower development and an obtaining method thereof. The gene sequence a has nucleotide base sequences represented by SEQ ID NO:1-6 in the sequence table. The obtaining method comprises the following steps: A, selecting a material for testing, B, extracting genome DNA, C, carrying out a long-distance PCR amplification reaction and detecting the obtained product, D, obtaining a specific amplification sequence, E, obtaining a candidate SNP, and F, verifying the candidate SNP locus, and finally obtaining the gene sequence a for causing watermelon bisexual flower development. According to the present invention, F2 generation segregation population dCAP molecule marker detection and phenotypic identification, and each core germplasm resource dCAP molecule marker detection and phenotypic identification are performed to confirm that the SNP locus is a co-segregation marker; and the obtained SNP locus can be used to develop genetic markers for assisted breeding so as to achieve a purpose of obtaining of the watermelon female line variety.

The invention discloses a novel animal cell expression vector containing a MAR (Matrix Attachment Region) core fragment. A nucleotide base sequence of the MAR core fragment of the recombinant expression vector is shown as SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The expression vector can be used for effectively improving the yield of protein in mammalian cells and the production cost is reduced.