35 results about "Nucleotide base sequence" patented technology

The invention belongs to the fields of a gene sequence and an obtaining method of the gene sequence, and particularly relates to an obtaining method of an SNP (single nucleotide polymorphism) site and a CAPS (cleaved amplified polymorphic sequence) mark interlocked with a citrullus lanatus fruit bitter taste gene Bt (bitterness). The SNP site and the CAPS mark interlocked with the citrullus lanatus fruit bitter taste have nucleotide base sequences in SEQ ID NO:1-2 in a sequence table. The citrullus lanatus fruit bitter taste gene is primarily positioned by screening a bitter taste single plant in an RILs colony and combining with the high-density genetic map information of the citrullus lanatus; the candidate SNP site interlocked with the character is combined with separate colony verification analysis and natural colony verification analysis; the mark tightly interlocked with a target character is obtained; the SNP site and the CAPS mark can be applied to improvement of citrullus lanatus variety and molecular assisted breeding; a technical support is provided for molecular breeding of the citrullus lanatus quality; and meanwhile, the traditional gene positioning time is greatly shortened.

The invention relates to the fields of gene sequences and acquiring methods thereof, and concretely relates to SNP loci linked with a blight resistant gene Fon-1 in a watermelon, markers thereof, and acquiring methods of the loci and the markers. The SNP loci linked with a blight resistant gene Fon-1 in a watermelon and the markers thereof have nucleotide base sequences represented by SEQ ID NO:1-8 in a sequence table. The acquiring method of the loci comprises the following steps: 1, selecting a tested material; 2, carrying out preliminary positioning of the blight resistant gene Fon-1 in the watermelon; 3, acquiring candidate SNP; and 4, acquiring the SNP loci closely linked with the blight resistant gene Fon-1 in the watermelon, and verifying the candidate SNP loci. According to the invention, the phenotype identification and the molecular detection of F2 generation separation populations and natural populations are carried out through utilizing a series of markers to confirm the close linkage degree of the series of the SNP loci with target properties; and the SNP loci are utilized to design the markers respectively, and the markers can be utilized to carry out the initial-stage screening of the kind in order to reach a molecule assisted breeding purpose, so the breeding period is substantially shortened, and an important scientific research meaning is possessed.

The invention belongs to the field of gene sequences and obtaining methods thereof, and particularly relates to a gene sequence a for causing watermelon bisexual flower development and an obtaining method thereof. The gene sequence a has nucleotide base sequences represented by SEQ ID NO:1-6 in the sequence table. The obtaining method comprises the following steps: A, selecting a material for testing, B, extracting genome DNA, C, carrying out a long-distance PCR amplification reaction and detecting the obtained product, D, obtaining a specific amplification sequence, E, obtaining a candidate SNP, and F, verifying the candidate SNP locus, and finally obtaining the gene sequence a for causing watermelon bisexual flower development. According to the present invention, F2 generation segregation population dCAP molecule marker detection and phenotypic identification, and each core germplasm resource dCAP molecule marker detection and phenotypic identification are performed to confirm that the SNP locus is a co-segregation marker; and the obtained SNP locus can be used to develop genetic markers for assisted breeding so as to achieve a purpose of obtaining of the watermelon female line variety.

The invention discloses a novel animal cell expression vector containing a MAR (Matrix Attachment Region) core fragment. A nucleotide base sequence of the MAR core fragment of the recombinant expression vector is shown as SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The expression vector can be used for effectively improving the yield of protein in mammalian cells and the production cost is reduced.