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Gene sequence a for causing watermelon bisexual flower development and obtaining method thereof

A technology of hermaphroditism and watermelon, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as inability to measure standards, and achieve the effect of shortening the time

Active Publication Date: 2014-06-25
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, the accuracy of homologous cloning using the principles of comparative genomics is generally only about 50%.
Although it is an important means to obtain target genes, it cannot be used as the only measure

Method used

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  • Gene sequence a for causing watermelon bisexual flower development and obtaining method thereof
  • Gene sequence a for causing watermelon bisexual flower development and obtaining method thereof
  • Gene sequence a for causing watermelon bisexual flower development and obtaining method thereof

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Embodiment 1

[0032] This embodiment is the sequence of gene a leading to the development of hermaphrodite flowers in watermelon and its obtaining method.

[0033] The gene a leading to the development of bisexual flowers of watermelon has the nucleotide base sequences of SEQ ID NO: 1-6 in the sequence table.

[0034] The gene a leading to the development of hermaphrodite flowers of watermelon has a nucleotide base sequence translated as the amino acid residue sequence of SEQ ID NO: 7-8 in the sequence table.

[0035] In the sequence listing, SEQ ID NO: 1 is the sequence specifically amplified by long-distance PCR of the andromonoecious material;

[0036] In the sequence listing, SEQ ID NO: 2 is the sequence specifically amplified by long-distance PCR of dioecious materials;

[0037] In the sequence listing, SEQ ID NO: 3 is the sequence of the CDS region of the andromonoecious material;

[0038] In the sequence listing, SEQ ID NO:4 is the sequence of the CDS region of the dioecious materi...

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Abstract

The invention belongs to the field of gene sequences and obtaining methods thereof, and particularly relates to a gene sequence a for causing watermelon bisexual flower development and an obtaining method thereof. The gene sequence a has nucleotide base sequences represented by SEQ ID NO:1-6 in the sequence table. The obtaining method comprises the following steps: A, selecting a material for testing, B, extracting genome DNA, C, carrying out a long-distance PCR amplification reaction and detecting the obtained product, D, obtaining a specific amplification sequence, E, obtaining a candidate SNP, and F, verifying the candidate SNP locus, and finally obtaining the gene sequence a for causing watermelon bisexual flower development. According to the present invention, F2 generation segregation population dCAP molecule marker detection and phenotypic identification, and each core germplasm resource dCAP molecule marker detection and phenotypic identification are performed to confirm that the SNP locus is a co-segregation marker; and the obtained SNP locus can be used to develop genetic markers for assisted breeding so as to achieve a purpose of obtaining of the watermelon female line variety.

Description

technical field [0001] The invention belongs to the field of gene sequence and its obtaining method, and in particular relates to the gene a sequence leading to the development of hermaphrodite flowers of watermelon and its obtaining method. Background technique [0002] Watermelon (Citrullus lanatus) is an annual herbaceous plant belonging to Cucurbitaceae Cucumber, and is an important economic crop widely cultivated at home and abroad. my country has a history of growing watermelons since the Han Dynasty. It is one of the earliest countries in the world to cultivate watermelons, and it is also the country with the largest watermelon cultivation area and the highest yield in the world. [0003] Watermelon is a model crop for the study of plant sex differentiation mechanism. Studying watermelon sex mechanism is helpful for the research and development of plant sex differentiation, and can improve breeding efficiency and reduce breeding cost. The main sex types of watermelon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/60C12N9/88C12N15/10
Inventor 许勇纪高洁张海英郭绍贵任毅宫国义张洁孙宏贺
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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