cDNA overall length nucleotide sequence of human EBLN-1 gene and cloning method thereof

Abstract

The invention discloses a cDNA overall length nucleotide sequence of a human EBLN-1 gene and a cloning method thereof. According to the invention, the cloning method comprises the following steps of: respectively amplifying a 5' end fragment and a 3' end fragment of human EBLN-1 gene according to 5'RACE and 3'RACE technologies and sequencing; then, splicing to obtain the cDNA overall length nucleotide sequence of the human EBLN-1 gene according to the 3' end sequence, the 5' end sequence and a known cDNA part sequence; performing analysis of an opened reading frame and an untranslated region; and deducing to obtain an amino acid sequence which codes the EBLN-1 protein according to the opened reading frame. The cDNA overall length nucleotide sequence of the human EBLN-1 gene can be used for subsequently expressing a recombinant protein for preparing corresponding antibodies or performing structural biological study, for studying EBLN-1 gene function through RNA interference and overexpression or further studying the effect of the untranslated region in regulation of gene expression, and finally laying a foundation for illustrating the physiological action of EBLN-1 gene in human body and the relation with diseases, in particular mental diseases.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a human endogenous Borna-like N element-1 (EBLN-1) isolated from human oligodendroglioma cells The full-length cDNA sequence of the gene and its cloning method. Background technique [0002] Borna disease virus (BDV) is a non-segmented single-stranded negative-sense RNA virus first discovered in Germany in 1885. It is strictly neurotropic. Its genome encodes 6 viral proteins, nucleoprotein ( N) is the main infection marker and pathogenic protein. In 1996, German scientists successfully isolated BDV from patients with depression, proving that BDV infection also exists in humans (Bode L, et al. Mol Psychiatry. 1996). In recent years, a large number of epidemiological investigations at home and abroad have suggested that there may be a correlation between the occurrence of human mental diseases, including depression, and BDV infection. A concept was proposed. [0003...

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Problems solved by technology

[0005] Commonly used research strategies for new genes mainly include bioinformatics and in vivo expression analysis of new genes, research on gain-of-function and inactivation strategies, and research on interacting proteins of gene-encoded products, all of which depend on the structural model of the gene. So far, the full-length cDNA sequence of the EBLN-1 gene is still unclear, which seriously lags behind the subsequent research on the physiological function and pathological effects of EBLN-1

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