37 results about "Radial cartilage" patented technology

The invention relates to a kind of laminated gradient composite scaffold material based on the bionic structure and its preparation method. The said material has three or more layers of porous structure, comprising of hyaluronic acid, PLGA, PLA, collagen II and nano-hydroxyapatite (nano-HA) , beta- tricalcium phosphate ( beta-TCP). The upper layer is made by collagen II / hyaluronic acid or PLGA and PLA imitating the cartilage layer. With the counterfeit the calcified cartilage layer in the middle, it is one layer or multi- sublayer, made by nano-HA or beta-TCP with collagen II / hyaluronic acid or PLGA and PLA; the bottom is made of nano-HA or beta-TCP with collagen II or PLGA and PLA. From top to bottom, the content of inorganic material increases in its layers, about 0 to 60 mass percent of layers. The aperture of the scaffold material is 50 to 450 micron, with 70 to 93 percent porosity. The scaffold material made by this invention has an adjustable degradation rate, good mechanical and biocompatible properties, can adapt to culture with cartilage bone cells and compound with growth factor and small molecular or peptides, it can be used to repair cartilage simultaneously.

A composite for osteochondral defect repair includes a porous scaffold and a periosteal graft secured to a surface of the scaffold. The composite provides cartilage growth from autologous periosteum chondrogenesis. Biological resurfacing of large osteochondral defects, or a complete joint is feasible using the porous scaffold / autologous periosteal composite. The use of this composite eliminates the necessity of using normal cartilage surface as a donor site and its respective associated morbidity. In one form, the strong bone integration capacity of a porous metal (e.g., tantalum) scaffold and the high grade of integration observed from periosteal chondrogenesis into the normal cartilage eliminates the lack of chondral-chondral integration observed in the autologous osteochondral graft technique.

The invention discloses a method for preparing functional gradient hydrogel for bone-cartilage repair and belongs to the technical field of biological materials. Two parts of double-bond biomacromolecule pre-polymerization liquid with the same mass fraction are prepared firstly, then the pre-polymerization liquid is prepared into upper-layer cartilage repair pre-polymerization liquid containing repair factor nanoparticles and lower-layer subchondral bone repair pre-polymerization liquid containing calcium phosphorus nanoparticles, and based on suspension principle and optical polymerization reaction, the functional gradient hydrogel is formed through sedimentation and diffusion of nanoparticles, wherein the function of the functional gradient hydrogel changes along with the change of compositions and structure. The prepared bionic hydrogel has high biodegradability and biocompatibility, loaded growth factors are high in efficiency and slow release performance, cartilage and subchondral bone repair can be induced, bidirectional regeneration of bone-cartilage is achieved, and repair of cartilago articularis injuries is achieved.

The invention is directed to a process for making a tissue repair implant having a porous sponge-like structure to repair bone, cartilage, or soft tissue defects by producing a connective tissue homogenate from one or more connective tissues; mixing the connective tissue homogenate with a carrier solution to produce a connective tissue carrier; optionally mixing one or more natural or synthetic bone fragements with said connective tissue carrier to produce a tissue repair mixture; freezing or freeze-drying the tissue repair mixture to produce a porous sponge-like structure and create a three-dimensional framework to entrap the natural or synthetic bone fragments, treating the frozen or freeze-dried porous sponge-like structure with one or more treatment solutions to produce a stabilized porous sponge-like structure. A crudely fragmented connective tissue from one or more connective tissues is optionally mixed with the tissue repair mixture before freezing or freeze-drying. The tissue repair implant having a porous sponge-like structure is optionally combined with one or more bioactive supplements or one or more agents that have bioactive supplement binding site(s) to increase the affinity of growth factors, differentiation factor, cytokines, or anti-inflammatory agents to the tissue repair implant. The invention is further directed toward applying such tissue repair implant for tissue repair.

The invention provides a bionic bone cartilage composite scaffold and preparation technology and fixing method thereof. According to the structure of the coloboma position of the cartilage of patient, reverse engineering and CAD technology are used for designing the composite scaffold which is matched with the coloboma position; the composite scaffold comprises a biological ceramic bone scaffold, a polylactic acid fixing pile and a hydrogel cartilage scaffold; the light-cured biological ceramic bone scaffold is manufactured by indirect moulding; polylactic acid is perfused in the biological ceramic bone scaffold, and the biological ceramic bone scaffold is cured and polished in a vacuum mold casing machine, and then a biological ceramic / polylactic acid composite scaffold is obtained; the bionic bone cartilage composite scaffold is prepared by light-cured rapid moulding and hydrogel solution; the bionic bone cartilage composite scaffold is placed in the defect position by operation, and is drilled with a support for fixing; bone cement is poured into the support fixing hole, a polylactic acid fixing pile is hit into the fixing hole immediately, after solidification, the implantation of the bone cartilage composite scaffold is completed. The invention is used for repairing large-area bone / cartilage defect with good effect.

A portable surgical workstation for implant formation comprising a base with a central planar section. The central planar section has a plurality of tracks and a throughgoing slot with a recessed stepped surrounding surface formed on a bottom surface of the central planar section. A vise assembly mounted to the base comprises a fixed jaw member secured to the base, a traveling jaw member moveably mounted to the base and a fixed drive housing mounted to the base. The traveling jaw member has a plurality of rail members adapted to be slidably mounted in the central planar section tracks. The fixed drive housing has a threaded longitudinal bore which receives a threaded drive shaft, one end of the drive shaft being secured in the traveling jaw member to transport the traveling jaw member.

The invention discloses an osteochondral integrated repairing gradient hybrid support material and a preparation method thereof. The hybrid support material successively consists of a cartilage layer,a cartilage calcification layer and a calcification lower bone layer from top to bottom, wherein all layers are closely combined into a whole body by virtue of a 3D printing technology. The preparation method comprises the following steps: (1) separately preparing hybrid gel of each layer; (2) establishing an osteochondral integrated support model, and preparing a pre-gel support by virtue of a 3D printing technology; and (3) preparing the osteochondral integrated repairing gradient support by adopting a freeze drying technology. The integrated support prepared by the invention simultaneouslyhas a material gradient and a structure gradient, the cartilage layer, and the bone layer are closely connected into a whole body by virtue of the cartilage calcification layer, so that an effect forisolating the cell migration between the two layers can be realized; and by virtue of the 3D printing technology, the individualized tissue engineering support can be rapidly and precisely prepared according to the requirements of different patients, and the integrated construction of a complicated appearance and internal micro-structure of the support can be realized, and the application potential is great.

An osteochondral composite including a hydrogel with implanted biological cells joined to a porous supportive base at a mechanically-interlocked interface is disclosed. A method of fabricating the osteochondral composite and a method of treating damaged or diseased articular cartilage by implanting an osteochondral composite are also disclosed.

A method is disclosed that produces allografts from matrices typically containing demineralized bone matrix (DBM) powder, demineralized bone matrix gel, demineralized bone matrix paste, bone cement, cancellous bone, or cortical bone and mixtures thereof. The matrices are sterilized utilizing supercritical CO2 in the presence of a sterilizing additive and an entrainer such as an alkaline earth metal compound, preferably CaCO3. The resultant allograft materials have a reduced rate of rejection when used in allograft procedures including, bone, cartilage, tendon, and ligament grafting procedures.

The invention discloses a method for culturing an artificial cartilage with different curved surfaces or a bone cartilage under the action of rolling load and sliding load. The method comprises an incubator which contains O2 and CO2 and can perform temperature adjustment and cell culture, perfusion and other conditions which are used for keeping culture solution fresh, and active factors which are added to the culture solution. The method is characterized in that culture blanks with different curved surfaces are fixed on the bottom plate of a culture room; during the culturing process, a culture is subjected to the effect of the rolling load and the sliding load simultaneously, and the culture is cultured into cartilage tissues or bone cartilage complex tissues. The method has the advantages that under the effect of the rolling load and the sliding load, the loading way of the method is closer to the mechanical environment of the growth and the development of the cartilage tissues; and under the loading condition, the method is favor of the growth and the development of cells and tissues. An experiment shows that mucopolysaccharide secreted by cartilage cells under the effect of the rolling load and the sliding load is higher than the mucopolysaccharide secreted by the cartilage cells only under the effect of the rolling load. After being cultured, the cultures with different shapes can be taken as implants, and the method of the invention is applied in repairing the defect of the cartilage in different parts.

The invention discloses a bone-cartilage bidirectional function bracket based on cell 3D printing, and a preparation method thereof. The preparation method for the bone-cartilage bidirectional function bracket based on cell 3D printing comprises the following steps: S1: respectively preparing an osteogenesis ink substrate and a chondrogenesis ink substrate; S2: adding mesenchymal stem cells and naringin into the osteogenesis ink substrate to obtain osteogenesis ink, and adding the mesenchymal stem cells and a transforming growth factor beta into the chondrogenesis ink substrate to obtain chondrogenesis ink; S3: respectively printing the osteogenesis ink and the chondrogenesis ink to obtain a bracket, wherein the bracket comprises an osteogenesis side and a chondrogenesis side; S4: after the bracket is subjected to crosslinking treatment, obtaining the bone-cartilage bidirectional function bracket. According to the bone-cartilage bidirectional function bracket prepared with the preparation method, the combined regeneration of bone-cartilage can be simultaneously realized, personalized customization is realized, the bone-cartilage bidirectional function bracket is favorable for the tissue regeneration of joints, and therefore, damaged joints can be more favorably repaired.

The invention relates to a method for constructing regenerated nerve vascularized bones, cartilages, joints or body surface organs by a prefabricated female die bracket. Firstly, the female die bracket of a defected tissue or organ is printed in a three-dimension manner by using a computer assisted design / manufacturing technology; then, a surface of a periosteum and / or perichondrium is formed in a body through an operation, the female die bracket is placed and fixedly sutured on a germinal layer of the autologous periosteum and / or perichondrium to form a closed space; after the bones, cartilages, joints or body surface organs whose male die shape corresponds to a female die are regenerated in the space constructed by the female die and the germinal layer of the surface of the periosteum and / or perichondrium, a newly regenerated tissue is taken out, the female die bracket is removed, and the tissue is transplanted to the position of the defected tissue or organ to reconstruct. The newly generated tissue or organ prepared through autologous prefabrication has rich new vessels and nerves, does not contain any exogenous materials participating in tissue regeneration, is high in safety, accurate in shape at the same time, and quite similar to the normal tissue or organ.

The invention relates to a kind of laminated gradient composite scaffold material based on the bionic structure and its preparation method. The said material has three or more layers of porous structure, comprising of hyaluronic acid, PLGA, PLA, collagen II and nano-hydroxyapatite (nano-HA) , beta- tricalcium phosphate ( beta-TCP). The upper layer is made by collagen II / hyaluronic acid or PLGA and PLA imitating the cartilage layer. With the counterfeit the calcified cartilage layer in the middle, it is one layer or multi- sublayer, made by nano-HA or beta-TCP with collagen II / hyaluronic acid or PLGA and PLA; the bottom is made of nano-HA or beta-TCP with collagen II or PLGA and PLA. From top to bottom, the content of inorganic material increases in its layers, about 0 to 60 mass percent of layers. The aperture of the scaffold material is 50 to 450 micron, with 70 to 93 percent porosity. The scaffold material made by this invention has an adjustable degradation rate, good mechanical and biocompatible properties, can adapt to culture with cartilage bone cells and compound with growth factor and small molecular or peptides, it can be used to repair cartilage simultaneously.

There is described a multiphasic osteochondral scaffold for osteochondral defect repair, the scaffold comprising a bone phase and a cartilage phase, wherein the bone phase comprises a support matrix and the cartilage phase comprises a polymeric matrix, and the scaffold comprises a non-porous layer between the bone phase and the cartilage phase. Also described is a multiphasic osteochondral scaffold for osteochondral defect repair, the scaffold comprising a bone phase and a cartilage phase, wherein the bone phase comprises a support matrix and the cartilage phase comprises a polymeric matrix, and wherein the support matrix is tapered so that the dimensions of the support matrix are less at the lower end of the support matrix than at the upper end of the support matrix.

The present disclosure relates to a three-dimensionally (e.g., 3D) printed, surgically implantable tissue engineering scaffolds for promoting bone, vascular, and / or cartilage regeneration at osteochondral regions and a method for manufacturing the 3D printed surgically implantable tissue engineering scaffold. The 3D printed surgically implantable tissue engineering scaffold may be fabricated at least in part from a thermoplastic polyurethane (e.g., nTPU) composite via a rapid prototyping machine. In some cases, the three-dimensional shape of the fabricated tissue engineering scaffold may correspond to a three-dimensional shape of a tissue defect of a patient.

The invention discloses a preparation method of a natural tissue-derived epiphyseal cartilage combined bone acellular material, and belongs to the technical field of biological materials for tissue or organ repair and regeneration. The preparation method comprises the following steps: rinsing and repeatedly freezing and thawing epiphyseal cartilage combined bone at the distal femur of a young large white pig, and treating the epiphyseal cartilage combined bone with a PBS buffer solution containing TritonX-100, a PBS buffer solution containing SLES, a PBS buffer solution containing HTHOPS, a PBS buffer solution containing DNaseI and normal saline to obtain the decellularized epiphyseal cartilage combined bone material. After thorough decellularization treatment is carried out on the epiphyseal and cartilage combined bone material, the completeness of the epiphyseal and cartilage combined bone ECM is reserved to the maximum extent while cells with immunogenicity and cell content components are completely removed, and the epiphyseal and cartilage combined bone ECM has the capability of repairing osteochondral defects and can be used for treating orthopedic diseases caused by osteochondral defects.

Described herein are hydrogels attached to a base with the strength and fatigue comparable to that of cartilage on bone and methods of forming them. The methods and apparatuses described herein may achieve an attachment strength between a hydrogel and a substrate equivalent to the osteochondral junction. In some examples the hydrogel may be a triple-network hydrogel (such as BC-PVA-PAMPS) that is attached to a porous substrate (e.g., a titanium base) with the shear strength and fatigue strength equivalent to that of the osteochondral junction.

The invention relates to the technical field of artificial joints, in particular to a knee joint local prosthesis and a personalized design method thereof. According to CT scanning data of knee joints of multiple patients, prosthesis data models with multiple specifications and models and dense size intervals are obtained, and a real knee joint bone model database of the patients is established; comparing the target patient bone data with a data model of an established knee joint bone model database one by one, matching to obtain bone data with high similarity, and calling local intact bone data corresponding to the diseased region of the target patient; the covering prosthesis covers all the lesion bearing areas; and calculating the resection thickness of the femur resection, so that the thickness of the implanted knee joint local prosthesis is equal to the sum of the resection of the posterior femur and the normal femur cartilage. The problems that when an existing knee joint femoral condyle prosthesis is used, the osteotomy amount is large, the operation difficulty is large, anterior and posterior cruciate ligaments are not reserved, and the movement experience of a patient is poor are solved through local model data of the diseased region obtained through matching.

The invention relates to the field of medical scaffolds, and relates to a fibrin material beneficial to cartilage repair. The fibrin material is a fibrinogen scaffold gelated under thrombin, and PLGAin the scaffold can carry Ag ions for human body sterilization, and can be degraded. High concentration of fibrinogen improves the mechanical properties, and improves the degradation resistance of thescaffold material. Due to the existence of porous, the speed of material exchange and biological signal exchange is accelerated. The exchange rate of nutrients increases when the nutrients pass through the pores in the human body. When the porous fibrin cartilage repair material is transplanted into the body, the fibrin cartilage repair material has good compatibility with human bone tissue and good binding ability, and the regeneration of cartilage is realized. According to the present invention, in situ cartilage induced regeneration can be achieved by the method, the object of in situ regeneration and repair is achieved, and the method has good clinical application prospects. The current phenomenon that the injury of cartilage tissue can not be well repaired by collagen clinically is overcome.

The invention relates to a method of producing an elastin-reduced cartilage scaffold containing channels and / or lacunae, the method comprising the steps of providing an elastic cartilage sample and reducing elastin from said cartilage sample to produce said channels and / or lacunae. The invention further relates to an elastin-reduced cartilage scaffold. Said elastin-reduced cartilage scaffold can be used in a method of implantation in vivo, for repairment of cartilage, repairment of osteochondral defects and repairment of bone defects.

The invention relates to a preparation method and application of a tissue engineering biological scaffold, and belongs to the technical field of biological medicines. According to the invention, bioactive glass nanospheres and an acellular cartilage extracellular matrix are included; and the bioactive glass nanospheres and the acellular cartilage extracellular matrix are constructed into the composite scaffold. The composite scaffold not only has the advantages of a decellularized cartilage extracellular matrix scaffold, but also overcomes some defects of a pure decellularized cartilage scaffold, the biological activity of the scaffold is increased, meanwhile, the mechanical property of the scaffold is also increased, and the scaffold can better promote regeneration and repair of cartilage and osteocartilage. By utilizing the characteristics that the acellular cartilage extracellular matrix can be subjected to freeze-drying forming and chemical crosslinking, the defect that bioactive glass nanospheres are not easy to form as granular products is overcome, and the advantages of high specific surface area, solubility, ion slow release characteristic and the like of the bioactive glass nanospheres are fully utilized. The invention provides a good biological scaffold for tissue engineering.

The invention discloses a preparation method of a personalized biphase condylar scaffold. The preparation method comprises the following steps of separating a mandible digital model by collecting data; selecting data on the opposite side of a lesion area in the mandible digital model, mapping the data to the lesion area in a mirror symmetry manner to obtain a condylar head and neck prosthesis model, and designing and printing a bone phase scaffold according to a condylar tissue structure form; preparing a cartilage phase scaffold; and spraying a compact layer on the surface of the head of the bone phase scaffold, connecting the cartilage phase scaffold with the compact layer through a dissolution-adhesion process to form a compact layer bone-cartilage composite scaffold, constructing a condylar biphase composite scaffold, and after a position retaining handle is designed on the condylar biphase composite scaffold, constructing the personalized biphase bionic condylar scaffold. The problems that the condylar form cannot be recovered and complications of a bone supply area exist in autologous bone graft temporomandibular joint reconstruction are solved, and the problems that the material mechanical property and the degradation period of an existing prosthesis cannot meet clinical requirements are also solved.

Methods, systems, and devices for the treatment of osteochondral defects (OCDs) are disclosed. The disclosed method and systems include collecting surface data of a joint using image-free methods, generating a three-dimensional (3D) healthy bone model based on the surface data of the joint and a database of healthy bone anatomies, defining of the boundary of the OCD on the joint, generating a 3D implant model based on the 3D healthy bone model and the boundary of the OCD on the joint, manufacturing an implant based on the 3D implant model, generating an implantation plan, resecting the joint according to the implantation plan, and placing the implant into the resected cavity on the joint. The disclosed devices include two or more distinct segments with distinct structures optimized for bone or cartilage growth and configured to receive distinct injectable biologic enhancement for targeted bone or cartilage growth.

The invention provides a bionic osteochondral complex and a preparation method thereof. The bionic osteochondral complex includes a cartilage layer, a barrier layer and a subchondral bone layer, and the two sides of the barrier layer are respectively connected to the subchondral bone layer and the subchondral bone layer. The cartilage layer is connected. The bionic osteochondral complex provided by the present invention can be implanted in human cartilage damage sites, and the cartilage, subchondral barrier layer and subchondral bone can be repaired in an integrated way, and the formation can not only induce the migration of endogenous cells but also play a barrier function biomimetic osteochondral prosthesis.

The present invention provides implants useful for treating cartilage and / or osteochondral defects that comprise a plurality of scaffolds arranged in a multi-layer stacked configuration, wherein each scaffold comprises a mesh of polymer fibers and wherein the polymer fibers comprise gelatin, a plant-derived protein, e.g., zein protein, or a combination thereof. Methods for repairing a cartilage and / or an osteochondral defect using implants of the invention are also provided.

This invention provides methods for optimal implantation of a solid substrate for promoting cell or tissue growth or restored function in an osteochondral, bone or cartilage tissue in a subject in need thereof. The methods include selecting and preparing a solid substrate for promoting cell or tissue growth or restored function for implantation, which solid substrate has a length and width or thatpromotes a tight fit within the boundaries of the implantation site and is further characterized by a height sufficient such that when a first terminus of said solid substrate is implanted within a bone in a site for implantation, a second terminus of said solid substrate is at a height at least 2 mm less than an articular cartilage layer surface or is proximal to a tide mark region in said implantation site and optionally applying a biocompatible polymer layer to an apical surface of said implant, which layer does not exceed the articular cartilage layer surface in height. Tools for implementation of optimal positioning are described including a unique tool (5-120) for trimming cartilage at the implantation site.