40 results about "Human cartilage" patented technology

The invention provides a method for serum-free culture of human cartilage cells and a serum-free culture medium. The method for serum-free culture of cartilage cells includes an enzymatic treatment step for treating a human cartilage cell-containing tissue with a protease; an inhibitor-treatment step for, after the enzymatic treatment step, treating the tissue with an inhibitor for the aforesaid protease; and a culture step for, after the inhibitor-treatment step, culturing the tissue in a serum-free culture medium that contains kartogenin and / or SAG, ITS, FGF2 and hydrocortisone. A serum-free culture medium for culturing cartilage cells, the serum-free culture medium containing kartogenin and / or SAG, ITS, FGF2 and hydrocortisone.

A method for differentiation of osteoarthritis from rheumatoid arthritis and non-disease conditions in a sample, comprising measuring in the sample the concentration of human cartilage intermediate layer protein 2(CILP-2) in body fluids and more specifically, measuring in the sample the concentration of the N-terminal part of CILP-2 (2C1) or fragments thereof.

The present invention pertains to a trimeric antigen binding molecule comprising three fusion polypeptides, each comprising at least one antigen binding moiety fused to a trimerization domain derived from human cartilage matrix protein. In addition, the present invention relates to polynucleotides encoding such trimeric antigen binding molecules, and vectors and host cells comprising such polynucleotides. The invention further relates to methods for producing the trimeric antigen binding molecules of the invention, and to methods of using these trimeric antigen binding molecules in the treatment of disease.

The invention discloses a method for preparing a chitosan double-layer scaffold with coexistence of nano and micro structures, which belongs to the field of biomedical materials. It is the first to use phase separation technology to prepare three-dimensional micro-chitosan scaffold as the base layer, and use high-voltage electrospinning technology to spin nano-chitosan fibers on the surface of micro-chitosan, so as to obtain chitosan bilayer with nano-micro structure coexistence. layer bracket. The prepared double-layer scaffold has good mechanical strength and high porosity, and is suitable for cell adhesion, growth and differentiation. Among them, the surface chitosan nanofiber is suitable for the growth of chondrocytes, and the base layer micron chitosan scaffold is suitable for the growth of osteoblasts, and can be applied to the repair of human cartilage and hard bone joints.

The invention discloses a tissue engineering cartilage scaffold and a preparation method thereof. The preparation method comprises the following steps: step A: extract type II collagen; step B: use the type II collagen, hyaluronic acid, chondroitin sulfate and Prepare primary CII‑HA‑CS‑HAP three-dimensional cartilage scaffolds from hydroxyapatite; step C: use EDC / NHS system to cross-link the primary CII‑HA‑CS‑HAP three-dimensional cartilage scaffolds in step B, then freeze-dry to obtain CII ‑HA‑CS‑HAP Three-dimensional Cartilage Scaffold. The tissue engineered cartilage scaffold prepared by the present invention has the advantages of good biocompatibility, low toxicity, easy degradation, and non-toxic degradation products; the tissue engineered cartilage scaffold prepared by the present invention has a compact appearance and various performance parameters are suitable for human cartilage Tissue repair: The tissue engineered cartilage scaffold constructed in the present invention has a wide range of materials and is easy to shape, and can be used for large area wound repair.

The invention belongs to the technical field of cell engineering, and particularly relates to a preparation method of an immortalized human cartilage endplate stem cell line and use of the immortalized human cartilage endplate stem cell line. The invention aims to solve the technical problem of providing an effective preparation method for constructing the immortalized human cartilage endplate stem cell line. The technical scheme is as follows: the preparation method for constructing the immortalized human cartilage endplate stem cell line by adopting the lentiviral transfection technology comprises the following steps: a, constructing a SV40T antigen virus vector; b, carrying out gene transfection of a human endplate stem cell on SV40T antigen; and c, passaging and sieving to obtain the immortalized human cartilage endplate stem cell line. The invention further provides the use of the immortalized human cartilage endplate stem cell line prepared by the method in preparation of bone tissue engineering materials. The immortalized human cartilage endplate stem cell line provided by the invention can be applied to preparation of a tissue-engineered bone and clinically provides a seed cell of the bone tissue engineering low in price and strong in osteogenic capability for the patients with a series of long bone defects and bone nonunion.

There are provided, inter alia, methods and compounds for activating a proliferative program in competent adult chondrocytes.

The invention discloses a preparation method of a novel high-strength micro-gel composite hydrogel. According to the invention, acrylamide and 2-acrylamide-dimethylpropane sulfonic acid are adopted as main monomers; N-methylol acrylamide is adopted as a functional monomer; reactive micro-gel containing hydroxymethyl is obtained through inverse emulsion polymerization; the micro-gel particles are dispersed into the water solution of acrylamide and 2-acrylamide-dimethylpropane sulfonic acid, and free radical polymerization is carried out, such that a micro-gel composite polymer is obtained; and the micro-gel composite polymer or a partially dehydrated micro-gel composite polymer is heated and is subjected to cross-linking, such that the micro-gel composite hydrogel is obtained. According to the invention, nano-clay is adopted as the cross-linking agent. The prepared composite hydrogel has higher mechanical strength, and high water absorption rate. The strength and water content of the hydrogel are similar to those of human cartilage tissues. Therefore, the hydrogel is an alternative material for cartilage tissue reparation.

The invention relates to the technical field of tissue engineering, and in particular relates to an in-vitro construction method of epiphyseal plate cartilage. The in-vitro construction method of the epiphyseal plate cartilage comprises the following steps: (1) culturing human cartilage cell line cells or human bone marrow stem cells till cell monolayers are formed, carrying out trypsinization, washing and resuspension to obtain a cell suspension, and carrying out counting for later use; (2) cutting a pretreated bone matrix gelatin material into a cylinder with the diameter smaller than the diameter of each culture hole, drilling a pore passage running through the two bottom surfaces of the cylinder in the cylinder, carrying out sterilization treatment, and fixing the cut bone matrix gelatin material in the culture holes in a suspension manner; and (3) implanting the cell suspension obtained in the step (1) to be below the bone matrix gelatin material from the pore passage, enabling the liquid level to wet the bone matrix gelatin material, and carrying out culturing to obtain the epiphyseal plate cartilage. With the adoption of the technical scheme, a culture system of C28 / I2 cells and an in-vitro construction method of the epiphyseal plate cartilage by adopting the culture system are established, and therefore, the problem that the seed cells are difficultly available is solved; the cells are stable in character and low in culturing cost.