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High-strength degradable cartilage tissue engineering scaffold and preparation method thereof

A cartilage tissue, high-strength technology, applied in the field of high-strength degradable cartilage tissue engineering scaffold and its preparation, chitosan/gelatin composite porous scaffold for cartilage repair and reconstruction and its preparation field, can solve the problem of unknown degradation performance, mechanical properties and poor performance. Biocompatibility is not up to the problem, to achieve the effect of controllable degradation rate, high strength, high porosity

Active Publication Date: 2015-06-24
NINGBO MEDICAL CENT LIHUILI HOSPITACL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanical properties and biocompatibility of the scaffold still cannot meet the requirements of cartilage tissue engineering, etc., and the degradation performance is unknown.

Method used

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  • High-strength degradable cartilage tissue engineering scaffold and preparation method thereof
  • High-strength degradable cartilage tissue engineering scaffold and preparation method thereof
  • High-strength degradable cartilage tissue engineering scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Dissolve 2ml acetic acid in 100ml deionized water to form a 2% acetic acid solution by volume percentage, then dissolve 4g chitosan and 4g gelatin in 2% acetic acid solution by volume to form the final mass volume of chitosan ratio of 4% and gelatin mass volume ratio of 4% mixed solution, mix evenly and seal, place in 50°C water bath and stir at 100 rpm for 24h until a light yellow homogeneous and stable solution is formed, stand for 12h for defoaming , to obtain chitosan / gelatin composite solution;

[0031] (2) Take 5 mL of chitosan aqueous solution with a mass percentage content of 5% and inject it into the mold, soak in a sodium hydroxide solution with a mass percentage content of 5% for 10 minutes to form a layer of inner film with a thickness of 0.5 mm, and then remove the mold;

[0032] (3) Fill the film with chitosan / gelatin mixed solution, put it into the same coagulation solution for 12 hours, then remove the prepared gel stick and put it into distilled wa...

Embodiment 2

[0040] Same as Example 1, but the addition of gelatin in step (1) becomes 2g, and others remain unchanged.

[0041] The chitosan / gelatin porous composite scaffold obtained in this embodiment is a composite scaffold in which the weight ratio of chitosan and gelatin is 2:1, wherein the mass percentage concentration of chitosan is 4%, and the mass percentage concentration of gelatin is 2%. .

Embodiment 3

[0043] Same as embodiment 1, but the add-on of gelatin becomes 8g in the step (1), other changeless.

[0044] The chitosan / gelatin porous composite scaffold obtained in this embodiment is a composite scaffold in which the weight ratio of chitosan and gelatin is 1:2, wherein the mass percentage concentration of chitosan is 4%, and the mass percentage concentration of gelatin is 8%. .

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Abstract

The invention discloses a high-strength degradable cartilage tissue engineering scaffold and a preparation method thereof. The high-strength degradable cartilage tissue engineering scaffold is formed by compounding chitosan and gelatine in weight ratio of 1:(0.01-2), wherein mass percent concentration of chitosan is 3-6%, and mass percent concentration of gelatine is 0.03-10%. The preparation method of the high-strength degradable cartilage tissue engineering scaffold is characterized in that natural biological materials such as chitosan and gelatine are adopted as raw materials, an in-situ precipitation and freeze-drying method is adopted for preparing a chitosan-gelatin compound porous scaffold which has excellent mechanical performance, controllable degradation rate and excellent biocompatibility, is not added with a crosslinking agent and is composed of pure natural biological materials. The chitosan-gelatin compound porous scaffold has the advantages that a degradation period can be regulated according to proportional relation between gelatine and chitosan, and growth rate matching probability of regenerated tissues is improved; and experiments verify that human cartilage cells can be well adhered and propagated on the surface of the materials.

Description

technical field [0001] The invention belongs to the field of new biomedical materials and its preparation, and relates to a high-strength degradable cartilage tissue engineering scaffold and a preparation method thereof, in particular to a chitosan / gelatin composite porous scaffold for cartilage repair and reconstruction and a preparation method thereof. Background technique [0002] Trauma, tumors, birth defects and other reasons may cause tissue damage and loss. How to repair and rebuild normal tissues and organs is a very important point in regenerative medicine. The emergence of tissue engineering provides the possibility to solve this problem, and a key in tissue engineering is the preparation of three-dimensional porous scaffold materials for tissue engineering using cell culture. In this process, the scaffold material not only plays a supporting role and maintains the shape of the original tissue, but also acts as a template, providing a place for cells to lodge, grow...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/56A61L31/04A61L31/14
Inventor 沈志森崔翔付俊
Owner NINGBO MEDICAL CENT LIHUILI HOSPITACL
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