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Engineering mechanically functional human cartilage and method of making same

An engineered, human technology used in pharmaceutical formulations, prostheses, joint implants, etc.

Inactive Publication Date: 2017-09-26
THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

None of these approaches produced mechanically functional human cartilage

Method used

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  • Engineering mechanically functional human cartilage and method of making same
  • Engineering mechanically functional human cartilage and method of making same
  • Engineering mechanically functional human cartilage and method of making same

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Embodiment

[0067] Bone marrow-derived human mesenchymal stem cells were obtained from Cambrex with fresh human bone marrow aspirates. Bone marrow-derived human mesenchymal stem cells (hMSCs) were isolated by attaching them to a plastic surface. Cells were expanded in DMEM supplemented with 10% FBS, 1% pen-strep, and 0.1 ng / mL basic fibroblast growth factor. hMSCs were cultured to the third passage and shown to exhibit multi-lineage differentiation capacity.

[0068] Generation of condensed mesenchymal bodies (CMBs) hMSCs were suspended in culture medium (supplemented with 100 nM dexamethasone; 50 μg / ml ascorbate-2-phosphate; 100 μg / ml sodium pyruvate; 40 μg / ml proline ; 1% insulin, transferrin, sodium selenite [ITS+] mixture; 1% antibiotic and 10 ng / ml transforming growth factor-β3 [TGF-β3] in high glucose DMEM). To determine the optimal size of CMBs, hMSCs were divided into 10 5 , 2.5×10 5 , 5×10 5 , 106, 1.5×10 6 and 2×10 6 cells / ml concentration. Add 1ml of cell suspension to ...

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Abstract

Medical devices having engineered mechanically functional cartilage from adult human mesenchymal stem cells and method for making same.

Description

[0001] Statement Regarding Federally Sponsored Research [0002] This invention was made with government support under Grant Nos. DE016525 and EB002520 awarded by the National Institutes of Health. The government has certain rights in this invention. technical field [0003] The present disclosure relates to the field of tissue engineering, and more particularly to tissue engineered mechanofunctional human cartilage. Background technique [0004] Knee and hip replacements are estimated to have multiplied over the years as the world population ages. Additionally, musculoskeletal injuries, such as sports injuries, are on the rise, with the pediatric population more than doubling in the past decade. [0005] Generating functional tissue from a patient's cells in vitro would revolutionize regenerative medicine by providing biological replacements for tissue lost or damaged due to injury, disease, or aging. The growing understanding of embryonic development and stem cell biolo...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61F2/30
CPCA61L27/3834A61L27/50A61L2430/06A61L27/46A61L27/56A61L2400/06A61L2430/10A61F2/30756C08L89/06A61F2/30749A61L27/3654A61L27/3662A61L27/3683A61F2002/30766A61F2/3094A61F2002/30762A61L27/3608A61L27/3612A61L27/425
Inventor S·普密拉塔纳G·武尼亚克-诺瓦科维奇
Owner THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK
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