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A kind of tissue engineered cartilage scaffold and preparation method thereof

A tissue engineering and cartilage technology, applied in the field of tissue engineering cartilage scaffold and its preparation, can solve problems such as unsatisfactory treatment effect, and achieve the effects of convenient application, good biocompatibility, and compact appearance.

Active Publication Date: 2017-10-13
河北太美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen that there are obvious deficiencies in the methods and means of conventional treatment of articular cartilage injuries, and the treatment effect is not ideal.

Method used

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  • A kind of tissue engineered cartilage scaffold and preparation method thereof
  • A kind of tissue engineered cartilage scaffold and preparation method thereof
  • A kind of tissue engineered cartilage scaffold and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation method of tissue engineered cartilage scaffold of the present invention comprises the following steps:

[0034] Step 1: Extraction of type II collagen.

[0035] First, select fresh bovine cartilage, remove the periosteum with chloroform and methanol, and pulverize the fresh bovine cartilage. Next, the pulverized cartilage tissue was stirred with 0.05 mol / L Tris-HCl containing 4 mol / L guanidine hydrochloride solution, centrifuged at 4° C., and the supernatant was removed. Repeat several times to remove proteoglycan. Then, the precipitate obtained by centrifugation was fully washed with 0.1-0.5 mol / L acetic acid, dissolved in acetic acid of the same concentration, and pepsin was added to digest the precipitate for 10-18 hours, stored at 4°C and centrifuged, and the centrifuged The supernatant was then digested for 5-8 hours, and the supernatant obtained above was combined to prepare a type II collagen solution. Finally, the above supernatant was salted ...

Embodiment 2

[0047] The difference between this example and Example 1 is that in Step 3, the CII-HA-CS-HAP three-dimensional cartilage primary scaffold was soaked in a mixed solution of ethanol and MES for 30 min at room temperature. Then, soak the primary three-dimensional cartilage scaffold in a mixed solution containing ethanol, MES, NHS, and EDC, and place it in an incubator at 25° C. for 36 hours for cross-linking. The various conditions of other experimental steps are kept unchanged, and the tissue engineering three-dimensional cartilage scaffold of the present invention is obtained.

Embodiment 3

[0049] The difference between this example and Example 1 is that in Step 3, the CII-HA-CS-HAP three-dimensional cartilage primary scaffold was soaked in a mixed solution of ethanol and MES for 30 min at room temperature. Then, soak the primary three-dimensional cartilage scaffold in a mixed solution containing ethanol, MES, NHS, and EDC, and put it in an incubator at 22° C. for 30 hours for cross-linking. With other conditions unchanged, the tissue engineered cartilage scaffold of the present invention is finally obtained.

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Abstract

The invention discloses a tissue engineering cartilage scaffold and a preparation method thereof. The preparation method comprises the following steps: step A: extract type II collagen; step B: use the type II collagen, hyaluronic acid, chondroitin sulfate and Prepare primary CII‑HA‑CS‑HAP three-dimensional cartilage scaffolds from hydroxyapatite; step C: use EDC / NHS system to cross-link the primary CII‑HA‑CS‑HAP three-dimensional cartilage scaffolds in step B, then freeze-dry to obtain CII ‑HA‑CS‑HAP Three-dimensional Cartilage Scaffold. The tissue engineered cartilage scaffold prepared by the present invention has the advantages of good biocompatibility, low toxicity, easy degradation, and non-toxic degradation products; the tissue engineered cartilage scaffold prepared by the present invention has a compact appearance and various performance parameters are suitable for human cartilage Tissue repair: The tissue engineered cartilage scaffold constructed in the present invention has a wide range of materials and is easy to shape, and can be used for large area wound repair.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a tissue engineering cartilage scaffold and a preparation method thereof. Background technique [0002] Articular cartilage is an essential component of joint function. It is composed of bone tissue and its surrounding perichondrium, while cartilage tissue is composed of chondrocytes, matrix and fibers. Cartilage plays an important role in joint function. However, articular cartilage damage is a very common disease. About 80% of patients undergoing arthroscopic surgery for various reasons have cartilage damage, and 20% have severe cartilage damage. Due to the lack of vascular tissue in the cartilage, and the cartilage tissue cells are wrapped in the matrix components, they cannot migrate to the damaged site to repair the defect, resulting in poor self-healing ability of the articular cartilage after injury, and regeneration cannot be achieved. If cartilag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/12A61L27/50
Inventor 周晓辉冯成宝刘浩
Owner 河北太美生物科技有限公司
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