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Induction method for promoting differentiation of human umbilical cord mesenchymal stem cells into chondrocytes

A technology of mesenchymal stem cells and chondrocytes, applied in the field of biomedicine, can solve problems such as source limitation and difficulty in obtaining a large number of bone marrow cells, and achieve the effects of improved survival rate, good cryopreservation effect, and high expression rate

Inactive Publication Date: 2019-05-31
和携科技有限公司
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Problems solved by technology

[0004] MSCs were originally found in bone marrow. Because the collection of bone marrow-derived MSCs requires bone marrow aspiration, the source is limited, and it is very difficult to obtain a large number of bone marrow cells.

Method used

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  • Induction method for promoting differentiation of human umbilical cord mesenchymal stem cells into chondrocytes

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Embodiment 1

[0024] A method for promoting the differentiation of human umbilical cord mesenchymal stem cells into chondrocytes, which sequentially includes the steps of obtaining umbilical cord tissue blocks, the step of primary culture of stem cells, the step of subculture of stem cells and the step of inducing the differentiation of stem cells into chondrocytes.

[0025] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the umbilical cord tissue. Tong's jelly tissue was rinsed repeatedly, and cut into pieces of umbilical cord tissue of ...

Embodiment 2

[0030] An induction method for promoting the differentiation of human umbilical cord mesenchymal stem cells into chondrocytes, which sequentially includes the steps of obtaining umbilical cord tissue blocks, the step of primary culture of stem cells, the step of subculture of stem cells, the step of cryopreservation of stem cells, and the recovery of stem cells With the step of culturing and the step of induction of differentiation of stem cells into chondrocytes.

[0031] The steps of obtaining the umbilical cord tissue block include sterilizing the ligated umbilical cord at both ends, and placing it in physiological saline containing 1wt% double antibody; The umbilical cord tissue segment was repeatedly rinsed with normal saline containing 1wt% double antibody to remove blood; the umbilical cord tissue segment was cut open, blood vessels and epidermal tissue were removed to obtain Wharton's jelly tissue, and normal saline containing 1wt% double antibody was used to treat the ...

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Abstract

The invention relates to an induction method for promoting differentiation of human umbilical cord mesenchymal stem cells into chondrocytes, which comprises the following steps of: obtaining an umbilical cord tissue block, a primary culturing stem cells, subculturingstem cells and inducing stem cells differentiate to chondrocytes, wherein the induction for differentiation of stem cellsinto chondrocytes comprises the following steps of: resuspending human umbilical cord mesenchymal stem cells subcultured to a seventh generation by adopting a serum-free culture medium to prepare P7 generation stem cell suspension; inoculating P7 generation stem cell suspension into the serum-free culture medium containing a cartilage differentiation inducer for continuous culture; replacing the serum-free culture medium containing the cartilage differentiation inducer in a half amount every three days; obtaining chondrocytes after 14 days.The induction method can differentiate human umbilical cord mesenchymal stem cells into chondrocytes,which has important significance in repairing human cartilage tissues and treating bone injuries.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to an induction method for promoting the differentiation of human umbilical cord mesenchymal stem cells into chondrocytes. Background technique [0002] Knee cartilage injury is one of the main research fields of sports medicine clinical work, and it is a hot spot and focus of sports science research. In the early stage of articular cartilage damage, it can cause pain and limitation of joint function of the injured joint. In the late stage, the joint may have degeneration and non-inflammatory osteoarthritis, which will seriously reduce the quality of life of the patient. After articular cartilage is damaged, chondrocytes mainly rely on synovial fluid and the matrix around the cells to provide nutrition, and supply energy through anaerobic glycolysis. Their own repair ability is very limited, and they cannot even be repaired by themselves. [0003] The emergence of mesenchymal s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077A01N1/02
Inventor 赵进军谷广其杨桂花赵宇飞李张鹏
Owner 和携科技有限公司
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