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Preparation method of clinical-grade human umbilical cord mesenchymal stem cell complex factor and lyophilized powder for repairing

A technology of stem cells and compound factors, which is applied in the fields of biochemical equipment and methods, animal cells, lyophilization and transportation, etc., can solve the problem that the medium is expensive and difficult to realize the productization of human umbilical cord mesenchymal stem cells compound factors and lyophilized powder. and other issues, to achieve the effect of safety and effectiveness, saving manpower and reducing immunogenicity

Pending Publication Date: 2018-11-16
陕西神州生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to avoid risks such as uncertain components and allergies and infections caused by heterogeneous serum, some scholars use commercially available serum-free medium with defined components and add a combination of exogenous factors: growth factors (such as bFGF, EGF, etc.), transferrin, albumin, etc. protein, trace elements and other components to compensate for the nutritional needs of cells in the serum-free culture process, but the commercially available medium is expensive, and it is difficult to realize the commercialization of human umbilical cord mesenchymal stem cell complex factors and freeze-dried powder

Method used

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  • Preparation method of clinical-grade human umbilical cord mesenchymal stem cell complex factor and lyophilized powder for repairing
  • Preparation method of clinical-grade human umbilical cord mesenchymal stem cell complex factor and lyophilized powder for repairing
  • Preparation method of clinical-grade human umbilical cord mesenchymal stem cell complex factor and lyophilized powder for repairing

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Experimental program
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Effect test

Embodiment Construction

[0049] (1) Preparation of clinical grade stem cell complete medium

[0050] (1) Preparation of clinical-grade stem cell basal medium: mix the various amino acid solutions, vitamin solutions and glucose solutions used in clinical applications at a volume ratio of 69:1:20. After mixing, add 100 mg / L ascorbic acid. The following table shows the detailed composition of CRSCBM.

[0051] Table 1 Detailed composition table of CRSCBM

[0052]

[0053]

[0054] (2) Preparation of self-extracted platelet lysate: Collect machine-collected platelets that have been tested to meet clinical blood standards, mix and resuspend in sterile normal saline, centrifuge at 200g at room temperature for 10 minutes, and clean residual impurities. After cleaning, adjust the platelet concentration to 1×10 9 / ml, and place it at -80°C overnight. The next day, take it out and place it in a 37°C water bath to re-thaw, and repeat the freeze-thaw operation three times to make it fully lysed and release growth factor...

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Abstract

The invention provides a preparation method of a clinical-grade human umbilical cord mesenchymal stem cell complex factor and lyophilized powder for repairing. The preparation method comprises preparation of a clinical-grade stem cell complete culture medium, extracting and culturing human umbilical cord mesenchymal stem cells, preparing the human umbilical cord mesenchymal stem cell complex factor, lyophilizing the human umbilical cord mesenchymal stem cell complex factor and the like. The prepared human umbilical cord mesenchymal stem cell complex factor and lyophilized powder do not containallogeneic serum components; the components of the culture medium are determined and the human umbilical cord mesenchymal stem cells have a rapid cell multiplication speed when being cultured by utilizing a culture medium system and have a strong bioactive substance secretion capability; the production cost is extremely reduced, so that the human umbilical cord mesenchymal stem cell complex factor and the lyophilized powder are suitable for industrialized large-scale production and can be applied to clinical wound healing and repairing type biomedicine products.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for preparing a clinical grade human umbilical cord mesenchymal stem cell composite factor for repair and a freeze-dried powder. Background technique [0002] Human umbilical cord mesenchymal stem cells (human umbilical cord mesenchymal stem cells, hUC-MSCs), as a kind of mesenchymal stem cells widely present in neonatal umbilical cord tissue, have the potential for self-renewal and multidirectional differentiation. Studies have shown that the therapeutic potential of hUC-MSCs can be attributed to three important mechanisms: 1. Homing, hUC-MSCs can migrate to acute injury sites through chemotaxis; 2. hUC-MSCs can differentiate into a variety of cells and tissues Types, such as cardiomyocytes, vascular endothelial cells, nerve cells, smooth muscle cells and skeletal muscle, cardiac muscle, fat, etc.; 3. Secretion of biologically active factors, which affect the local or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61K9/19A61P17/02
CPCA61K47/26A61P17/02A61K9/19A61K35/28C12N5/0665C12N2500/05C12N2500/38C12N2500/34C12N2500/32C12N2500/84C12N2501/998
Inventor 孙蓓屈佩瑶聂苏秦
Owner 陕西神州生物技术有限公司
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