Preparation method for stem cell extract-rich degradable biofilm for wound repair

A wound repair and stem cell technology, applied in the field of biomedicine, can solve the problems of materials that are difficult to degrade, rejection and invasion, and poor biocompatibility

Inactive Publication Date: 2016-12-07
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemically synthesized materials have strong mechanical properties, but the materials themselves are difficult to degrade and have poor biocompatibility. Implantation in the body may cause rejection and invasive reactions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step (1), adding penicillin concentration to L-DMEM medium is 100U / mL, streptomycin concentration is 100U / mL, is mixed with bone marrow mesenchymal culture fluid;

[0027] Step (2), under aseptic conditions, rinse the bone marrow cavity of the rat with the bone marrow mesenchymal culture solution configured in step (1), add the washed out bone marrow into a sterile culture bottle, and add the step ( 1) The bone marrow mesenchymal culture solution prepared in 1), the volume ratio is 1:1, and the bone marrow cells are fully blown away with a sterile pipette to prepare a bone marrow cell suspension;

[0028] Step (3), centrifuging the bone marrow cell suspension obtained in step (2) at 800r / min for 5min, discarding the supernatant to obtain the bone marrow stem cell population;

[0029] Step (4), take the bone marrow stem cell population obtained in step (4) and add it to the culture medium prepared in step (1) to resuspend and filter it with a 70 μm sterile nylon filter, ...

Embodiment 2

[0034] Step (1), adding penicillin concentration to L-DMEM medium is 100U / mL, streptomycin concentration is 100U / mL, is mixed with bone marrow mesenchymal culture fluid;

[0035] Step (2), under aseptic conditions, rinse the bone marrow cavity of the rat with the bone marrow mesenchymal culture solution configured in step (1), add the washed out bone marrow into a sterile culture bottle, and add the step ( 1) The bone marrow mesenchymal culture solution prepared in 1), the volume ratio is 1:1, and the bone marrow cells are fully blown away with a sterile pipette to prepare a bone marrow cell suspension;

[0036] Step (3), centrifuging the bone marrow cell suspension obtained in step (2) at 1500r / min for 10min, discarding the supernatant to obtain the bone marrow stem cell population;

[0037] Step (4), take the bone marrow stem cell population obtained in step (4) and add it to the culture medium prepared in step (1) to resuspend and filter it with a 70 μm sterile nylon filter...

Embodiment 3

[0042] Step (1), adding penicillin concentration to L-DMEM medium is 100U / mL, streptomycin concentration is 100U / mL, is mixed with bone marrow mesenchymal culture fluid;

[0043] Step (2), under aseptic conditions, rinse the bone marrow cavity of the rat with the bone marrow mesenchymal culture solution configured in step (1), add the washed out bone marrow into a sterile culture bottle, and add the step ( 1) The bone marrow mesenchymal culture solution prepared in 1), the volume ratio is 1:1, and the bone marrow cells are fully blown away with a sterile pipette to prepare a bone marrow cell suspension;

[0044] Step (3), centrifuging the bone marrow cell suspension obtained in step (2) at 1200r / min for 7min, discarding the supernatant to obtain the bone marrow stem cell population;

[0045]Step (4), take the bone marrow stem cell population obtained in step (4) and add it to the culture medium prepared in step (1) to resuspend and filter it with a 70 μm sterile nylon filter, ...

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PUM

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Abstract

The invention discloses a preparation method for a stem cell extract-rich degradable biofilm for wound repair. The preparation method comprises the following steps: adding extracted bone marrow into a mesenchymal stem cell culture solution, discarding supernatant after centrifugation, then performing inoculation in a culture dish at 1*109 / mL, performing culturing in an incubator, and inoculating third-generation mesenchymal stem cells into a fiberboard laid with a fibrin film to prepare a mesenchymal stem cell-fibrin film. According to the preparation method, mesenchymal stem cells are separated and cultured, the fibrin film is used as a stent material, and the in-vitro cultured mesenchymal stem cells and the fibrin film are combined and transplanted to a cartilage damaged part to achieve the effect of repairing a damaged cartilage. The mesenchymal stem cells can be better limited by the fibrin film to be grown and reproduced on the surface of a cartilage damaged wound so as to play an important role in a cartilage wound repair process more effectively and play a certain guiding role in human cartilage damage repair.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a method for preparing a degradable biofilm rich in stem cell extract for wound repair. Background technique [0002] Bone marrow mesenchymal stem cells have become the focus of life science research in the 21st century. They can self-proliferate and differentiate in multiple directions, and have extremely broad application prospects. At present, bone marrow mesenchymal stem cells have achieved great results in animal experiments and clinical applications, and have made breakthroughs in bone or cartilage damage, heart disease, central nervous system damage, liver damage, spinal cord damage, etc., and it is not like Human embryonic stem cells also involve ethical and moral issues. There are many methods for isolating bone marrow mesenchymal stem cells. So far, no specific cell marker has been found to identify bone marrow mesenchymal stem cells, and bone marrow mesenchymal stem cells h...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/38A61L27/58C12N5/0775
CPCA61L27/225A61L27/3834A61L27/3852A61L27/58A61L2430/06C12N5/0663
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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