The invention relates to an extraction kit and method for genome DNA of evergreen woody plants and belongs to the field of genetic engineering. The extraction kit comprises a rinsing liquor, a lysate, a leaching liquor A, a leaching liquor B, a binding buffer liquor, a column buffer liquor, an elution buffer liquor and an adsorption column, and further comprises a grinding assisting agent, wherein the grinding assisting agent comprises insoluble PVPP, quartz sand and ascorbic acid; the rinsing liquor comprises Tris-HCl, EDTA-Na2, NaCl, KAc, glucose, PVP, ascorbic acid and PEG; and the lysate comprises CTAB, Tris-HCl, EDTA-Na2, NaCl, sorbitol, borax, PVP, ascorbic acid and protease K. The extraction method is flexible and quick in operation, low in cost and wide in application range, and can radically remove secondary metabolites and other interfering substances in cells, and the extracted genome is good in quality, high in purity and less in protein pollution.