70 results about "Intracellular substance" patented technology

A method for producing AAV, without requiring cell lysis, is described. The method involves harvesting AAV from the supernatant. For AAV having capsids with a heparin binding site, the method involves modifying the AAV capsids and / or the culture conditions to ablate the binding between the AAV heparin binding site and the cells, thereby allowing the AAV to pass into the supernatant, i.e., media. Thus, the method of the invention provides supernatant containing high yields of AAV which have a higher degree of purity from cell membranes and intracellular materials, as compared to AAV produced using methods using a cell lysis step.

Green tea formulations and methods for the preparation thereof are disclosed and described. Generally speaking, the method of preparation includes the mixing of fresh tea leaves in an amount of cold water, followed by pulverization of the leaves to release their intracellular material from the cells of the green tea leaves into the water and form an aqueous extract component. The remaining cellular material forms a leaf residue component which is removed from the mixture. Once the leaf residue is removed, the aqueous extract component is collected and may be dried or further processed to produce a final tea extract that has good natural color, robust natural flavor, and pleasant organoleptic properties, which also is high in polyphenol content, and may be used for various purposes such as the creation of a green tea beverage.

A process and system for electrical extraction of intracellular matter from biological matter, and intracellular matter products formed thereby, based on preparing a mixture of biological matter featuring cells, and an electro-conductive liquid, and electrifying the mixture by transmitting controlled cycles of pulses and pauses of electrical current into the mixture by using electrodes, whereby the pulses of electrical current pierce holes into or perforate the cell membranes of the cells, enabling the release of intracellular matter for collecting and separating into target intracellular matter extract and solid waste. Pauses included in each cycle of transmitting pulses of electrical current enable firm control of electrical extraction processing conditions, including extent of extraction, temperature effects, and pressure effects, during the electrical extraction process. Electrical extraction of the present invention is applicable to a wide variety of biological matter originating from human, animal, and plant entities. Intracellular matter extracts so obtained include highly concentrated liquid fertilizer, valuable elements, nutrients, oils, and fats, which are used for manufacturing a diversity of end products of the agricultural, pharmaceutical, cosmetic, and food industries.

Provided are surprisingly beneficial osmotic stress shock methods for facilitating disruption and / or extraction of microorganisms, comprising a synergistic combination of at least one hypotonic shock and at least two hypertonic shocks, wherein the last shock is a hypertonic shock. Preferred aspects of the invention relate to facilitating disruption of a broad spectrum of microorganisms (e.g., algae, bacteria, yeast, fungus, etc.). In particular aspects the microbial cells are subjected to: a hypertonic / hypotonic / hypertonic tertiary shock; a hypotonic / hypertonic / hypertonic tertiary shock; or a hypotonic / hypertonic / hypotonic / hypertonic quaternary shock. Particular aspects further comprise disrupting and / or extracting of the shocked microbial cells, and isolating a cellular constituent or bioproduct (e.g., biofuel, biocrude, bioenergy, biogas, biodiesel, bioethanol, biogasoline, pharmaceuticals, nutraceuticals, food, vitamins, feedstock, dyes, colorants, sulfur, fertilizer, bioplastic) therefrom. In particular preferred aspects, facilitation of algae disruption and / or extraction is provided and in certain embodiments, isolation of at least one of lipid, oil, and triacylglycerol is enhanced.

The invention relates to a multistage anaerobic digestion strengthened gas production method mainly comprising the steps of front-stage anaerobic digestion, high-temperature and high-pressure thermal hydrolysis and back-stage anaerobic digestion. After sludge is subjected to the front-stage anaerobic digestion treatment, easily-degraded organic matters are sufficiently decomposed and converted into marsh gas, the residual part mainly comprises difficultly-biodegraded organic matters and facultative bacteria which adapt to the anaerobic environment and can not be digested and utilized, and the organic matters account for over 50% of the total organic matters in the sludge and restrict the development of anaerobic digestion. The discharged material subjected to the front-stage anaerobic digestion is subjected to the high-temperature and high-pressure thermal hydrolysis treatment to force a cell wall to crack, substances in a cell to be dissolved out and a colloid structure of an extracellular polymer to be broken, so that parts of difficultly-biodegraded organic matters are converted into the easily-degraded organic matters; the sludge subjected to the thermal hydrolysis treatment is added into a back-stage anaerobic digestion system to further realize the degradation and anaerobic gas production of the organic matters. By using the multistage anaerobic digestion strengthened gas production method, the degradation rate and gas production rate of the organic matters in the sludge are radically increased, the quantity and volume of the sludge are reduced, the sludge stabilizing effect is enhanced, and the quality of anaerobic digestion sludge is improved.

The invention relates to an intra-unicellular substance detecting method based on a whispering gallery mode, and belongs to the fields of laser technologies and biophotonics. The method comprises thefollowing steps: (1) whispering gallery mode microcavity selection is performed; (2) microcavity and cell are treated; (3) a concrete implementation method of detection on biological substances to betested is performed; (4) the detection ranges of the detecting method involve numerous specific biological substances such as nucleic acid, protein, cells and biomolecules. The detection sensitivity is high; the detection can be realized in cells. By the whispering gallery mode, fluorescence can form resonant vibration in the cavity; laser is generated; signals are greatly enhanced; the tracking detection time is prolonged.

The present invention relates to cell membrane-derived nanovesicles, a method of preparing the same, and a pharmaceutical composition and a diagnostic kit using the nanovesicles. The cell membrane-derived nanovesicles according to the present invention may prevent the occurrence of potential side effects because intracellular materials (e.g., genetic materials and cytosolic proteins) unnecessary for delivery of therapeutic or diagnostic substances are removed from the nanovesicles. In addition, since the nanovesicles may be targeted to the specific types of cells or tissues, therapeutic or diagnostic substances may be predominantly delivered to the targeted cells or tissues while delivery of therapeutic or diagnostic substances to untargeted sites may be inhibited. Therefore, when the cell membrane-derived nanovesicles are applied to disease treatment, the side effects of therapeutic substances such as drugs may be reduced, so that suffering and inconvenience of patients may be alleviated during the course of treating diseases, and therapeutic efficacy may be improved. In addition, the cell membrane-derived nanovesicles of the present invention, in which substances for the treatment or diagnosis of diseases are loaded, and a method of preparing the nanovesicles may be used in vitro or in vivo for therapeutic or diagnostic purposes, or for experimental use.

The present invention belongs to the field of microbial fermentation technology, and particularly to a micro ecological feed additive for regulating rumens and a preparation method thereof. The additive consists of metabolites and intracellular substances of yeast, and metabolites of aspergillus oryzae with a mass ratio of 15: 1, 20: 1 or 25: 1. Single strain industrial fermentation technology is used to produce a specific anti-stress metabolite composite, the method is stable in process, and effectively solves the defects that viable organism and enzyme preparations are easily inactivated and unstable, which greatly expands the application range of the additive, and is conducive to achieve the targeted product development of different animals and different physiological stages of the animals. The additive is not a viable organism preparation, but is the specific anti-stress metabolite composite produced by viable organisms after high density fermentation, and the additive is easy to store so that active ingredient activity cannot be decreased with prolonged storage time, which ensures active ingredient contents of the additive.

A method for measuring cytoplasm viscosity based on quantum dot three-dimensional tracing is disclosed. The method includes firstly introducing quantum dots into cells through a lipid Lipo-2000 transfection manner to achieve cell labeling; subjecting the single and recognizable quantum dots dispersed in the cells to three dimensional positioning and tracing by adopting an optical modulating mannerthrough a build quantum dot three-dimensional imaging system; further solving mean square displacement values MSDs which are motion parameters of the quantum dots by utilizing three-dimensional motion information; selecting quantum dots meeting free diffusion characteristics according to mathematical characteristics of the MSDs; finally solving diffusion coefficient D of the quantum dots in cytoplasm according to the MSD values for all the quantum dots meeting free diffusion characteristics; and further acquiring the cytoplasm viscosity according to a Stokes-Einstein equation. In a state thatcells survive, the cytoplasm viscosity can be accurately acquired by the method, visual three-dimensional motion information of molecules in cytoplasm can be obtained, and technical bases for researching intracellular material transportation, signal transmission, metabolism, differentiation, and the like are provided.

A cell analysis apparatus is described that includes a test sample preparation part for preparing a test sample by treating an analyte with a dye for staining an intracellular substance of the cells in the analyte, a detector for detecting scattered light and fluorescence from each cell in the test sample and a controller for identifying living mononuclear cells based on the scattered light and the fluorescence detected by the detector. A cell analysis method is also described.

The invention belongs to the field of sludge treatment and disposal, in particular relates to a process of a sludge decreasing method by effectively combining a chemical oxidation technology and a biotechnology. The process comprises the following steps: (1) in an ozone preoxidation treatment unit: generating strong oxidization by dosing ozone to damage hardly biodegradable cell membranes, and the like, rapidly dissolve matters in the cell in water, oxidize insolvable macromolecular matters to be easily utilized by microorganisms and be favorable to further biodegradation; (2) in a membrane bioreactor unit: synergistically strengthening to remove organic matters released by the crushed sludge by utilizing the self digestion of the sludge and the technologies of biodegradation, membrane filtration, and the like in the membrane bioreactor so as to achieve the sludge decreasing aim and also ensure that the effluent quality accords with the emission standard or the industrial reuse water quality requirement. The process has the advantages of good sludge decreasing effect, no secondary pollution, low energy consumption, direct effluent drainage, and the like.

A method of cell imaging based on using laser to induce fluorescent light collects fluorescent light emitted from dye - labeled cell by objective lens of microscope under shine of laser to be cell be image in CCD monitor through filter and then to obtain photograph of the cell. The light path portion of device for realizing the method consists of laser, shutter, extender lens, reflector, prism, objective lens of microscope, trap filter, band - pass filter and CCD monitor.

The invention discloses an extraction method for artemisia argyi essential oil. The method comprises the following steps: raw material pretreatment, microwave assisted extraction, compound enzyme assisted extraction, steam distillation extraction, condensation and refining. According to the method, artemisia argyi leaves are placed in distilled water for ultrasonic cleaning, so that impurities such as protein and glucose can be simply, conveniently and effectively removed from surfaces of the artemisia argyi leaves, the generation of the impurities during essential oil extraction is reduced, the aim of improving purity of the essential oil is achieved, and extraction cost increase resulting from traditional solvent cleaning and the influence on the purity of the essential oil caused by solvent residual are avoided. Then, microwave assisted extraction, compound enzyme assisted extraction, steam distillation extraction and molecular distillation are combined, cell wall rupture and localswelling of intercellular substances are achieved by microwave assisted extraction and compound enzyme assisted extraction, and thus, intracellular substances are more easily dissolved out; subsequently, the essential oil is thoroughly refined by steam distillation extraction; finally, refining is carried out by molecular distillation, the separation of lightweight molecules from heavy molecules is achieved by dint of difference among mean free paths of molecular movement of different substances, and thus, the purity of the artemisia argyi essential oil is greatly improved.

The invention which belongs to the technical field of drinking water treatment especially relates to a method for reinforcing the coacervation algae removal and controlling the release of intracellular substances in the process of high algae water treatment. The invention which aims at the eutrophication pollution of surface water sources of lakes, reservoirs and the like provides a method for improving the algae cell removal effect of technologies of coagulation, deposition and filtration of a water work with the combined action of potassium permanganate and a ferric salt, wherein the appropriate oxidation effect of potassium permanganate inactivates and inhibits the activity of algae cells and controls the release of intracellular metabolites, and In situ MnO2 which is in situ generatedthrough a reduction reaction of potassium permanganate deposits on the surface of the algae cells to improve the settlement performance of the algae cells; and the electrical neutralization of the ferric salt allows the potential of the surface of the algae cells to be improved, and In situ Fe(OH)3 which is in situ generated through a hydrolysis reaction of the ferric salt deposits on the surfaceof the algae cells to improve the potential and the settlement performance of the surface of the algae cells. The method of the invention which is mainly applied to the purification in the drinking water work can also be applied to landscape water treatment which aims at the landscape water reuse and the water source pretreatment of the eutrophication water to inhibit the growth of the algae cells.

The invention discloses a method for extracting okra pectin by high-voltage pulse and belongs to the technical field of okra pectin extraction. The extraction method uses the fresh and tender okra asthe raw material, the fresh and tender okra is subjected to enzyme inactivation, enzymatic hydrolysis, high-voltage pulsed electric field treatment and alcohol precipitation treatment in turn. The preparation method is simple, the high-voltage pulsed electric field belongs to non-heat treatment technology, can destroy plant cell membrane successfully, increase dissolution rate of intracellular substance, and has the advantage of improving extraction rate and maintaining performance stability of okra pectin.

The invention discloses a material with a function of antisepsis in cooperation with bacterial adhesion prevention and preparation and application thereof. According to the material with the functionof antisepsis in cooperation with bacterial adhesion prevention and the preparation and application thereof, based on the character that an organic antibacterial agent is lasting in antibacterial effect, the material surface is modified by means of the performance of antisepsis in cooperation with bacterial adhesion prevention of the special structure of organic molecules, wherein the super-hydrophobicity of vinyl silicone oil is used for reducing bacterial adhesion force to make bacteria attached to the material surface easy to remove; the property is used that an amphipathic vinyl nitrogen-containing compound can act on cytomembrane to result in release of materials in cells, so that bacterial growth is inhibited; the material surface which is modified by bonding the two compounds effectively inhibits bacterial adhesion, growth and reproduction. The prepared material has a good effect of antisepsis in cooperation with bacterial adhesion prevention and is lasting in effect of antisepsis in cooperation with bacterial adhesion prevention; in experiments, after the material (especially fabric) prepared by means of the method provided by the invention is washed for 30 times, 97% or higher of the antisepsis rate and 96% or higher of the bacterial adhesion prevention rate can still be maintained.

Intracellular material is released from bacterial, yeast, plant, animal, insect or human cells by the application of a low voltage such as 1 to 10 V to a suspension containing the cells. The conditions may be selected such that DNA released from the cells is electrochemically denatured so as to be available for use in an amplification procedure.

The invention relates to a method for high-speed mechanical cyclone separation of activated sludge biomass. The biomass comprises extracellular substances and intracellular substances. The method comprises the following steps: high-speed rotating mechanical force directly acts on activated sludge, and meanwhile, hydraulic cyclone is formed; and under the dual action of crushing of high-speed mechanical force and hydrocyclone, activated sludge biomass is separated and eluted. According to the method, a high-speed mechanical cyclone method is adopted, so that a mechanical, hydraulic, temperatureand chemical comprehensive effect is formed under the assistance of hot alkali conditions, extracellular substances in the activated sludge are emphatically separated and eluted, and the separation,elution, hydrolysis and extraction efficiency of the activated sludge biomass is greatly improved.

The invention relates to a straw feed and a preparation method thereof. The preparation method comprises the following steps of culturing extremely halophilic archaea-halogranum amylolyticum TNN58; after the culturing of thalli is completed, performing cell pyrolysis by a water pyrolysis method. After centrifugal treatment, solids mainly comprise biological degradable plastics PHBV and part of intracellular substances, and precipitates can be used for PHBV extraction; supernatant contains a large quantity of nutrient substances, so that when the supernatant is added to straw, the content of nutrient substances such as protein in the straw can be effectively increased, and the nutrient value of the straw can be increased.

An apparatus for quantitative identification of distinctive cellular events occurring in a cell population using a non-fluorescence approach. The apparatus comprises an image acquisition unit having a Differential Interference Contrast microscope with a camera, a light source, an environmental chamber allowing carrying out cell culture of at least one cell in a cell population; the image acquisition unit acquiring images of the cell population, at predetermined time points; a cell tracker for individually tracking the at least one cell of the cell population in the images; a distinctive cellular event detector for detecting an occurrence of a distinctive cellular event; a report generator; wherein the distinctive cellular event is selected from the group consisting of: tripolar, tetrapolar, quadpolar cell division, cell fusion, cell death, impaired cell division, cell shape alteration, nuclear shape alteration, inner cellular material accumulation, cell enlargement, engulfing, hyper-mobilization, hypo-mobilization and prolonged doubling time.

The invention belongs to the field of tea beverages, and particularly relates to a rocking green tea processing technology. The technology comprises the following steps: raw material preparation, sunning, withering, rocking, enzyme deactivation, rolling, and drying. According to the rocking green tea prepared by the invention, a special solution 1 is added during withering, and tea is irradiated with red light so as to improve the permeability of tea cells; and then an additive is added to react with substances in the cells during the rocking, so that more aroma substances are generated, and tea soup is green with yellow, and has a fresh feeling and heavy flower fragrance and fruit fragrance; and meanwhile, the leaching of beneficial components of the tea is increased, wherein the contentof water extract is up to 40.31%, and the content of tea polyphenols is up to 36.2%, and the leaching efficiency of amino acid is up to 56.2%, and all leaching factors effectively improve the qualityof the green tea.

The invention discloses an improved tetrandrine extraction method, which comprises the following technical steps: carrying out high-concentration ethyl alcohol alkalifying extraction, carrying out acid dissolution, carrying out D72 macropore cation exchange resin separation, carrying out methylbenzene extraction, and carrying out alternating crystallization on acetone and ethyl alcohol to obtain aproduct. According to the extraction method, the cavatition effect of ultrasonic wave is used for breaking the cells of plant medicines, and therefore, a solvent can easily penetrate into cells. Meanwhile, the strong vibration of the ultrasonic wave can be used for transferring huge energy to leached medicines and solvent to enable the medicines and the solvent to move at a high speed, and the releasing, the diffusion and the dissolving of intracellular substances can be enhanced. Compared with a traditional extraction method, the method has the advantages of high efficiency, high selectivity, simpleness in operation, high yield and the like, and a product can be easily purified.

The invention relates to a method for making goat liver paste. The method is characterized by comprising the following steps: grinding a goat liver with a colloid mill multiple times, performing ultrasonic treatment for multiple times, stirring, homogenizing, heating, curing, deodorizing, sterilizing, modulating, enabling cells of the goat liver to break, releasing substances in the cells, turning into the biological molecular state and becoming opaque and viscous goat liver liquid. By adopting the method, the defects of coarse mouthfeel, bad smell, difficulty in uniforming taste matching, inapplicability for industrial large-scale production and the like of the traditional making method can be overcome. The processing process can change the characters of the goat liver, break the cells, enable the substances contained in the cells to overflow, enable molecules to become small, remove the bad smell and peculiar smell, be good in delicate mouthfeel, be applicable to large-scale processing and production, be uniform in formula and be uniform in standard; and equipment is simple, the process is simple, the processing is simple, and the operation is simple, thereby having good practicality and feasibility.

The invention discloses saccharomyces cerevisiae SITCC No. 20012 with a collection number of CCTCC M 2017367; the DPPH free radical removal rates in intracellular substances and metabolites of the saccharomyces cerevisiae are 3.2 percent and 30.6 percent. The saccharomyces cerevisiae SITCC No. 20012 is used for preliminary fermentation of a fruit enzyme product; Shanghai brewed 1.01 acetic bacteria is used for secondary fermentation; the alcohol content of the obtained fruit enzyme product is less than 0.5 percent; the DPPH free radical removal rate of the obtained fruit enzyme product is increased by at least 40 percent when being compared with that of a naturally fermented fruit enzyme product; the saccharomyces cerevisiae SITCC No. 20012 has an extremely good application prospect in thefruit enzyme product.

The invention discloses an acinetobacter strain for low-temperature denitriding and an application thereof and relates to an acinetobacter strain and an application thereof. The acinetobacter strain is preserved in China General Microbiological Culture Collection Center on September 1, 2014 and has the preservation number of CGMCC No. 9627. Since the acinetobacter strain bd-01 disclosed by the invention is a heterotrophic nitrification bacterium and can have a heterotrophic nitrification reaction at a low temperature of 6 DEG C so as to convert ammoniacal nitrogen into nitric nitrogen, nitrite nitrogen and intracellular substances. By adopting the acinetobacter strain, the total ammonia can be efficiently and thoroughly removed and the degradation rate of nitrogen is100% at the low temperature of 6 DEG C within 48 hours. The acinetobacter strain for low-temperature denitriding can be used for denitriding domestic sewage, agricultural wastes and industrial wastewater in areas with a low-emperature environment.