The invention belongs to the field of
biotechnology, and particularly relates to a respiratory syncytial
virus (RSV)
virus-like particle (VLPs)
subunit vaccine as well as a preparation method and application thereof. The component of the vaccine is chimeric
antigen protein which is fusion-expressed together with neutral antigenic
epitope of an RSVG
protein or simultaneously with the antigenic
epitope of T cells of an M2
protein by taking
hepatitis B
virus core protein as a carrier. The high-purity
antigen component is prepared by efficiently expressing
antigen protein in
escherichia coli and then performing in-vitro purification, degeneration and renaturation and
self assembling into virus-like particles (VLPs). The RSVG protein contained in the VLPs and the antigenic
epitope of the T cells of the M2 protein are simultaneously expressed, so that the capability of the vaccine for introducing
specific immunity response and anti-RSV infection
immunity protection can be enhanced, the balanced Th1 / Th2
immunity response can be induced and the RSV vaccine is prevented from enhancing the incidence of diseases. Animals are immunized and inoculated with VLPs vaccines to induce organisms to generate high-level RSV neutral antigens, enhanced Th1
cell factor level and effective protection on RSV
attack infection.