The invention provides a
cryopreservation method of primary-generation mouse parenchymal liver cells. The method includes the steps of firstly, preparing mouse parenchymal liver
cell cryopreservationliquid; secondly, using a two-step
perfusion method to separate the mouse parenchymal liver cells; thirdly, using the
cryopreservation liquid to resuspend the mouse parenchymal liver cells, and then adding the mouse parenchymal liver cells into a
cryopreservation tube; fourthly, using a step-by-step temperature reduction method to cryopreserve the mouse parenchymal liver cells; fifthly, resuscitating the mouse parenchymal liver cells; sixthly, using a dye exclusion method to measure the
survival rate of the mouse parenchymal liver cells; seventhly, determining
enzyme index activity; eighthly,observing the morphology of the mouse parenchymal liver cells; ninthly, performing
ELISA method detection; tenthly, performing RT-PCR detection. The cryopreservation method has the advantages that themethod is simple to operate, many cells can be obtained after cryopreservation resuscitating,
high survival rate is achieved, and the method is an ideal primary-generation mouse parenchymal liver
cell cryopreservation method and capable of providing reliable
cell resources for experiments.