35 results about "Dose-effect curve" patented technology

The invention relates to an inducing and breeding method of high yield neutral proteinase strain by using low energy N <+> ion injection technology. The steps are that (1) the initial strain is screened; (2) the N <+> ion is injected for the induced mutation; (3) the high yield strain is screened; (4) the N <+> ion is injected for the induced mutation; (5) the high yield neutral proteinase strain is determined. The invention uses the N <+> ion injection technology for the induced mutation of Bacillus Subtilis AS1.398 of neutral proteinase; after the injection of different dosage of N <+> ion, the survival rate of the strain takes a typical saddle shape dose-effect curve; a high yield mutant strain with good genetic stability can be screened at the best injection dosage of 50*10 <14> ions / cm <2>; the shaking flask activity of the neutral proteinase is about 8230U / mL, which is improved by 81.3 percent. The ion injection technology can be applied into the mutation and selection of high yield neutral proteinase strain; the invention has a higher mutation rate and a wider mutation spectrum for the microorganism; the invention has good mutation effects, which is an ideal breeding method for the microorganism.

The invention provides a radiation-sensitive gene marker and its application in different types of radiation dose monitoring. The radiosensitive gene markers include Mgmt, Bax, Thyn1 and Phlda3. The dose-response curve constructed based on the expression level of the radiation-sensitive gene marker can effectively determine the range of the radiation dose, so as to effectively monitor the dose of different types of radiation exposure, and then accurately indicate the different types of radiation exposure based on biological effects. Reference dose for nuclear radiation damage assessment.

The invention provides a radiation-sensitive gene marker and its application in X-ray radiation dose monitoring. The radiosensitive gene markers include three up-regulated gene markers and two down-regulated gene markers; the up-regulated gene markers include Phlda3, Fgg and Kng1; the down-regulated gene markers include Ptprb and Kti. The dose-response curve constructed based on the expression level of the radiation-sensitive gene marker can effectively determine the range of radiation dose, thereby effectively monitoring the level of X-ray exposure dose, and then accurately prompting the reference dose of X-ray exposure based on biological effects , for nuclear radiation damage assessment.

The invention relates to a cigarette smoke cell proliferation toxicity evaluation method based on bromodeoxyuridine (BrdU) doping. The method is characterized by comprising the following steps: (1) preparing a solution; (2) carrying out cell inoculation; (3) contaminating cigarette smoke and doping BrdU; (4) assaying the doped amount of BrdU; (5) resulting and analyzing. The method has the following characteristics that through preparing a smoke granule-phase matter extracting solution and a smoke gas-phase matter absorbing solution, the cell toxicity of granule-phase and gas-phase matters of smoke can be inspected respectively; by a cell applicability validation step, the method can be applicable to a variety of adherent culture cells and can be used for inspecting the cell proliferation toxicity to different cell lines caused by cigarette smoke; the sensitivity of detection is improved through optimizing the doping time of the BrdU and the concentration of cell membrane permeation liquid; the best contaminating concentration is determined through optimizing the contaminating concentration of the cigarette smoke, so as to obtain the optimal dose-effect curve. In addition, the method further has the advantages of quickness, simplicity and convenience in operation, high sensitivity and stable results.

The embodiment of the invention discloses a [Gamma] ray irradiation dose estimation method based on a transcription factor IIIA, and relates to the nucleonic field. The irradiation dose is scaled through the changing of the transcription factor IIIA divalent zinc ion concentration. The [Gamma] ray irradiation dose estimation method based on the transcription factor IIIA employs the chemical colorimetry to detect the transcription factor IIIA divalent zinc ion concentration of different doses [Gamma] ray irradiation, and a dose-effect curve between the transcription factor IIIA divalent zinc ion concentration and the irradiation dose is established. According to the established dose-effect curve, a double blind method is employed to estimate the [Gamma] ray irradiation dose, the dose estimation range is wide and the operation is simple.

The invention relates to an electronic cigarette smoke solution cell toxicity evaluation method based on lactate dehydrogenase (LDH) assaying. The method comprises the following steps: (1) preparing an experiment reagent; (2) carrying out cell inoculation; (3) contaminating an electronic cigarette smoke solution; (4) assaying LDH; (5) resulting and analyzing. According to the method, aiming at the characteristic that most of electronic cigarette smoke solution samples are high in density, a method that an electronic cigarette contaminated solution is prepared by adopting mass weighing is determined; the sensitivity of detection is improved through optimizing cell inoculating density, selecting a cell lysing solution and optimizing the reaction time of an LDH matrix solution; the best contaminating concentration is determined through optimizing the contaminating concentration of the electronic cigarette smoke solution, so as to obtain the optimal dose-effect curve; through assaying LDH, the degree of damage to cell membranes caused by the electronic cigarette smoke solution can be reflected, so that the cell toxicity mechanism of electronic cigarette is disclosed. In addition, the method further has the advantages of quickness, simplicity and convenience in operation, high sensitivity and stable results.

The invention discloses a method for determining dose-effect of a traditional Chinese medicine compound on the basis of variable importance in projection. Compared with the prior art, the research method for determining the dose-effect relationship of a traditional Chinese medicine compound on the basis of variable importance in projection has the advantages as follows: 1, the most important in-blood ingredient variable is acquired through analysis of in-blood ingredients with the method based on variable importance in projection, analysis of the in-blood ingredients is superior to that of ingredients of the compound, and a scheme calculated with the method is accurate and reliable; 2, according to the method, a threshold of a clinically recommended medicine is given on the basis that the dose-effect relationship is determined, valuable reference advice can be proposed for the clinic medicine, and direct cognition is provided for prediction of the clinic medicine through a dose-effect curve fitting equation; 3, according to the method, the in-blood ingredients of the traditional Chinese medicine compound are adopted for dose-effect analysis, so that the analysis result is more reasonable and accurate; 4, a result of the method is displayed through patterns, and accordingly, people can better understand the analysis result.

The invention discloses a method for testing the toxicity of residual nicosulfuron on corn. A group of nicosulfuron solutions, the concentrations of which are gradiently distributed, are sprayed in a group of tested soils, and corn seeds are sowed on the sprayed tested soils; on the first day after spraying, the in-situ pore water of all the soils is collected, and the nicosulfuron concentrationsin the in-situ pore waters are determined; after a plurality of days of culture, the plant heights of corn are determined; finally, a dose-effect curve equation for the plant heights and the nicosulfuron concentrations in the in-situ pore water and the nicosulfuron concentrations IC50 in the in-situ pore water at the corn plant height inhabitation rate of 50 percent are obtained by fitting; the nicosulfuron concentrations in the in-situ pore waters of the soils to be tested are determined, and according to the dose-effect curve equation, the plant height inhabitation rate of the soils plantedwith corn is predicted. The method is easy and rapid to operate, and can rapidly predict the phytotoxicity of nicosulfuron in soil to be tested according to the dose-effect curve equation of the nicosulfuron in the soil and the concentration of the in-situ pore water in the soil to be tested.

The invention discloses a WST-1 method for testing in-vitro cytotoxicity of tobacco juice of an electronic cigarette. The method is characterized by comprising the following steps: (1) preparing a laboratory reagent; (2) culturing cells; (3) inoculating cells; (4) contaminating the tobacco juice of the electronic cigarette; (5) dyeing through WST-1; and (6) obtaining a result and analyzing. Compared with the prior art, the method has the characteristics that a method for preparing an electronic cigarette contamination solution by adopting weighing is determined aiming at the characteristic of high density of most electronic cigarette tobacco juice samples, the sensitivity of detection is improved by optimizing the reaction time of the WST-1 dye liquor through an optimization step of a cell inoculation density, an optimal contamination concentration is determined by optimizing an electronic cigarette tobacco juice contamination concentration so that an optimal dosage effect curve can be obtained, and a test step of washing cells a plurality of times is omitted by virtue of WST-1 dyeing; the testing method also has the advantages of faster and simpler operation, higher sensitivity and more stable result.

The invention relates to a neutral red absorption assaying method for evaluating the cell toxicity of an electronic cigarette smoke solution. The method is characterized by comprising the following steps: (1) preparing an experiment reagent; (2) carrying out cell inoculation; (3) contaminating the electronic cigarette smoke solution; (4) dyeing with neutral red; (5) resulting and analyzing. According to the method, aiming at the characteristic that most of electronic cigarette smoke solution samples are high in density, a method that an electronic cigarette contaminated solution is prepared by adopting mass weighing is determined; by a cell inoculation density optimizing step, cells of a cell line BEAS-2B, which are most applicable to the toxicity evaluation of the electronic cigarette smoke solution under the optimal inoculation density, are screened; the best contaminating concentration is determined through optimizing the contaminating concentration of the electronic cigarette smoke solution, so as to obtain the optimal dose-effect curve. The assaying method disclosed by the invention further has the advantages of quickness, simplicity, convenience and high sensitivity.

The invention relates to the field of activity detection of biological medicine, in particular to a biological activity determination method for an anti-PD-L1 monoclonal antibody. According to the method, a PD1 / PD-L1 interruption detection system is used, binding of PD1 and PD-L1 protein is interrupted when the anti-PD-L1 monoclonal antibody is bound with PD-L1 expressed by target cells, a transcription factor NFAT is activated through signal transduction, expression of luciferase is started, the expression quantity of luciferase is positively correlated to the biological activity of the anti-PD-L1 monoclonal antibody, the chemiluminescence value of the anti-PD-L1 monoclonal antibody is determined after a cell lysis solution and a luciferase substrate are added, a dose-effect curve is drawn, and accordingly the biological activity of the anti-PD-L1 monoclonal antibody is determined. The method is easy to implement, strong in specificity and high in durability and has great significance on quality control and clinical application of the anti-PD-L1 monoclonal antibody.