Neutral red absorption assay method for e-cigarette liquid cytotoxicity evaluation

Abstract

The invention relates to a neutral red absorption assaying method for evaluating the cell toxicity of an electronic cigarette smoke solution. The method is characterized by comprising the following steps: (1) preparing an experiment reagent; (2) carrying out cell inoculation; (3) contaminating the electronic cigarette smoke solution; (4) dyeing with neutral red; (5) resulting and analyzing. According to the method, aiming at the characteristic that most of electronic cigarette smoke solution samples are high in density, a method that an electronic cigarette contaminated solution is prepared by adopting mass weighing is determined; by a cell inoculation density optimizing step, cells of a cell line BEAS-2B, which are most applicable to the toxicity evaluation of the electronic cigarette smoke solution under the optimal inoculation density, are screened; the best contaminating concentration is determined through optimizing the contaminating concentration of the electronic cigarette smoke solution, so as to obtain the optimal dose-effect curve. The assaying method disclosed by the invention further has the advantages of quickness, simplicity, convenience and high sensitivity.

Description

Technical field [0001] The invention relates to the determination of the cytotoxicity of the electronic cigarette liquid, in particular to a quantitative determination method based on the neutral red dye analysis of the cell survival rate after the electronic cigarette liquid is poisoned. Background technique [0002] As people understand that "smoking is harmful to health", governments of all countries have actively adopted and implemented effective legislative, administrative or other measures within the scope of current laws and regulations to prohibit smoking in public places. In 2005, China signed the World Health Organization's Framework Convention on Tobacco Control. In 2012, the Chinese government issued the "China Tobacco Control Plan (2012-2015)", which proposed to create a smoke-free environment and implement a total ban on smoking in public places. Indoor smoking bans have led to the rapid growth of new products such as electronic cigarettes in the markets of various...

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Problems solved by technology

However, whether this method can be used to evaluate the cytotoxicity of e-cigarette liquid has not been reported in the literature

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